LOCUS       40924_41949        5772 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSVhR55d243-383, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5772)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5772)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87835.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1638..2160)
                     /label=3' UTR of TNFRd
                     /note="3' UTR of TNFRd"
     CDS             complement(2161..3021)
                     /codon_start=1
                     /note="unnamed protein product; TNFRd"
                     /protein_id="SJL87836.1"
                     /translation="MVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPG
                     PGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYR
                     HYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECT
                     KLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKS
                     TPEKEGELEGTTTKPLAPNPSWREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPA
                     PSLLR"
     misc_feature    complement(3022..3108)
                     /label=sTNFR
                     /note="sTNFR"
     misc_feature    complement(3109..3189)
                     /label=5' UTR of TNFR
                     /note="5' UTR of TNFR"
     promoter        complement(3201..3530)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3945..4533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4707..5564)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5565..5669)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtaccg
     1621 agctcgaatt cgggcgggtg tatgtacaaa agtccacagc tccagctgaa ggccccattg
     1681 ttccgtgctc gcccctgcct taggacagtt cagcttgcta tgtgcttgtc caggcagagg
     1741 gcacaggagt gccaagtttc tattagtgta acatgattga tttaaaaaca aaacaaaaca
     1801 aaacaaaaac aaaaaaaact gcttatgcac tgtgaaaaag gctcagggac gaaccagggg
     1861 cccccgagca gccttgctgg tgaggacacc caaaacgggc atgaggcata gcgtccctca
     1921 tcctcgcaaa ccacccactc aggctcttga gcccacggcg cacctctctc cgcgcgcaca
     1981 gcgctgactg tcggcggcgc gcaggcagct gagaaaagct atgtacatcg aggggttagc
     2041 accaagtagg cggctgctag ctcctgcttg cccctgcagg acccctcctt tccagaaaaa
     2101 agtggggttg gaaggcgatc tcgcaggacg gtccttagag ctgcccgcag gggcgcagcc
     2161 tcatctgaga agactgggcg cgggcgggag ggcggcgggg ccgcaaagcg cctcctcgat
     2221 gtcctccagg cagcccagca ggtccatgtc gcggagcacg cgtcccagca gctccagcgt
     2281 ggcctcgcgc cagcttgggt ttggggccag gggcttagta gtagttcctt caagctcccc
     2341 ctctttttca ggtgtcgatt tcccacaaac aatggagtag agcttggact tccaccgttg
     2401 gtagcgatac attaaaccaa tgaagaggag ggataaaagg caaagaccaa agaaaatgac
     2461 caggggcaac agcactgtgg tgcctgagtc ctcagtgccc ttaacattct caatctgggg
     2521 taggcacaac ttcgtgcact ccaggctttt cttacagtta ctacaggaga cacactcgtt
     2581 ttctcttaga aagaaacctg catggcaggt gcacacggtg ttctgtttct cctggcagga
     2641 gaggtgcacg gtcccattga ggcagaggct gcaattgaag cactggaaaa ggttttcact
     2701 ccaataatgc cggtactggt tcttcctgca gccacacacg gtgtcccggt ccactgtgca
     2761 agaagagatc tccacctgac ccatttcctt tcggcatttg gagcagctga ggcagtgtct
     2821 gaggtggttt tctgaagcgg tgaaggagcc gctctcacac tccctgcagt ccgtatcctg
     2881 ccccgggcct ggacagtcat tgtacaagta ggttcctttg tggcacttgg tacagcaaat
     2941 cgaattattt tgagggtgga tatattttcc ttggggacac acactatctc tcttctccct
     3001 gtcccctagg tgagggacca gtccaataac ccctgagggg tatattccca ccaacagctc
     3061 caggagcacc agcggcagca gcaggtcagg cacggtggag aggcccatgc cagacagcta
     3121 tggcctctca ctcccccatt tgggctcagg gcagtgtggc agcggcagtg ctggggcttc
     3181 ccgggactca gcttgagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     3241 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     3301 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     3361 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     3421 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     3481 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagt
     3541 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
     3601 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
     3661 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
     3721 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gctcttccgc ttcctcgctc
     3781 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3841 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3901 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3961 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4021 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4081 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4141 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4201 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4261 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4321 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4381 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4441 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4501 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4561 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4621 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4681 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4741 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4801 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4861 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4921 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4981 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     5041 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5101 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5161 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5221 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5281 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     5341 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5401 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5461 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5521 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5581 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5641 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     5701 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     5761 cgtcttcaag aa
//
