LOCUS       40924_41944        5886 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSVhR55d203-304, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5886)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5886)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87883.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1638..2160)
                     /label=3' UTR of TNFR
                     /note="3' UTR of TNFR"
     CDS             complement(2161..3135)
                     /codon_start=1
                     /note="unnamed protein product; TNFRd"
                     /protein_id="SJL87884.1"
                     /translation="MVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPG
                     PGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYR
                     HYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECT
                     KLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIAIPNPLQKWEDSAHKPQSLDT
                     DDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTP
                     RREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR"
     misc_feature    complement(3136..3222)
                     /label=sTNFR
                     /note="sTNFR"
     misc_feature    complement(3223..3303)
                     /label=5' UTR of TNFR
                     /note="5' UTR of TNFR"
     promoter        complement(3315..3644)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(4059..4647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4821..5678)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5679..5783)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtaccg
     1621 agctcgaatt cgggcgggtg tatgtacaaa agtccacagc tccagctgaa ggccccattg
     1681 ttccgtgctc gcccctgcct taggacagtt cagcttgcta tgtgcttgtc caggcagagg
     1741 gcacaggagt gccaagtttc tattagtgta acatgattga tttaaaaaca aaacaaaaca
     1801 aaacaaaaac aaaaaaaact gcttatgcac tgtgaaaaag gctcagggac gaaccagggg
     1861 cccccgagca gccttgctgg tgaggacacc caaaacgggc atgaggcata gcgtccctca
     1921 tcctcgcaaa ccacccactc aggctcttga gcccacggcg cacctctctc cgcgcgcaca
     1981 gcgctgactg tcggcggcgc gcaggcagct gagaaaagct atgtacatcg aggggttagc
     2041 accaagtagg cggctgctag ctcctgcttg cccctgcagg acccctcctt tccagaaaaa
     2101 agtggggttg gaaggcgatc tcgcaggacg gtccttagag ctgcccgcag gggcgcagcc
     2161 tcatctgaga agactgggcg cgggcgggag ggcggcgggg ccgcaaagcg cctcctcgat
     2221 gtcctccagg cagcccagca ggtccatgtc gcggagcacg cgtcccagca gctccagcgt
     2281 ggcctcgcgc cgcggcgtgc gccgcctcca ggtcgccagc atgctgtatt gcgcctcgcg
     2341 caggcagcgc ccgttctgca gctccagccg atcgatctcg tggtcgctca gccctaggcg
     2401 ccgcacgaat tccttccagc gcaacggggg cacgttctcc accacggcgt acagcgtcgc
     2461 ggggtcatca gtgtctaggc tctgtggctt gtgggcgctg tcctcccact tctgaagggg
     2521 gttggggatg gcaatgaaga ggagggataa aaggcaaaga ccaaagaaaa tgaccagggg
     2581 caacagcact gtggtgcctg agtcctcagt gcccttaaca ttctcaatct ggggtaggca
     2641 caacttcgtg cactccaggc ttttcttaca gttactacag gagacacact cgttttctct
     2701 tagaaagaaa cctgcatggc aggtgcacac ggtgttctgt ttctcctggc aggagaggtg
     2761 cacggtccca ttgaggcaga ggctgcaatt gaagcactgg aaaaggtttt cactccaata
     2821 atgccggtac tggttcttcc tgcagccaca cacggtgtcc cggtccactg tgcaagaaga
     2881 gatctccacc tgacccattt cctttcggca tttggagcag ctgaggcagt gtctgaggtg
     2941 gttttctgaa gcggtgaagg agccgctctc acactccctg cagtccgtat cctgccccgg
     3001 gcctggacag tcattgtaca agtaggttcc tttgtggcac ttggtacagc aaatcgaatt
     3061 attttgaggg tggatatatt ttccttgggg acacacacta tctctcttct ccctgtcccc
     3121 taggtgaggg accagtccaa taacccctga ggggtatatt cccaccaaca gctccaggag
     3181 caccagcggc agcagcaggt caggcacggt ggagaggccc atgccagaca gctatggcct
     3241 ctcactcccc catttgggct cagggcagtg tggcagcggc agtgctgggg cttcccggga
     3301 ctcagcttga gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     3361 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     3421 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     3481 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     3541 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     3601 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagtcgggca
     3661 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
     3721 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
     3781 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
     3841 gtgtttcgta aagtctggaa acgcggaagt cagcgctctt ccgcttcctc gctcactgac
     3901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3961 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4021 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4081 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4141 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4201 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4261 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4321 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4381 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4441 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     4501 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4561 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4621 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4741 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4801 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4861 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4921 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4981 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5041 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5101 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     5161 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5221 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5281 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5341 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5401 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     5461 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5521 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5581 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5641 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5701 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5761 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     5821 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     5881 caagaa
//
