LOCUS       V002921                 6214 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002921
VERSION     V002921
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6214)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87875.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1629..2182)
                     /label="3' UTR of TNFR"
                     /note="3' UTR of TNFR"
     CDS             complement(2186..3550)
                     /gene="TNFRSF1A"
                     /label="Tumor necrosis factor receptor superfamily member
                     1A"
                     /note="Tumor necrosis factor receptor superfamily member 1A
                     from Homo sapiens. Accession#: P19438"
     misc_feature    complement(3551..3631)
                     /label="5' UTR of TNFR"
                     /note="5' UTR of TNFR"
     promoter        complement(3643..3972)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(4387..4975)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5149..6006)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6007..6111)
                     /label="AmpR promoter"
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtaccg
     1621 agctcgaatt cgggtttttt ttttaacttc agaattttag tgtatgtaca aaagtccaca
     1681 gctccagctg aaggccccat tgttccgtgc tcgcccctgc cttaggacag ttcagcttgc
     1741 tatgtgcttg tccaggcaga gggcacagga gtgccaagtt tctattagtg taacatgatt
     1801 gatttaaaaa caaaacaaaa caaaacaaaa acaaaaaaaa ctgcttatgc actgtgaaaa
     1861 aggctcaggg acgaaccagg ggcccccgag cagccttgct ggtgaggaca cccaaaacgg
     1921 gcatgaggca tagcgtccct catcctcgca aaccacccac tcaggctctt gagcccacgg
     1981 cgcacctctc tccgcgcgca cagcgctgac tgtcggcggc gcgcaggcag ctgagaaaag
     2041 ctatgtacat cgaggggtta gcaccaagta ggcggctgct agctcctgct tgcccctgca
     2101 ggacccctcc tttccagaaa aaagtggggt tggaaggcga tctcgcagga cggtccttag
     2161 agctgcccgc aggggcgcag cctcatctga gaagactggg cgcgggcggg agggcggcgg
     2221 ggccgcaaag cgcctcctcg atgtcctcca ggcagcccag caggtccatg tcgcggagca
     2281 cgcgtcccag cagctccagc gtggcctcgc gccgcggcgt gcgccgcctc caggtcgcca
     2341 gcatgctgta ttgcgcctcg cgcaggcagc gcccgttctg cagctccagc cgatcgatct
     2401 cgtggtcgct cagccctagg cgccgcacga attccttcca gcgcaacggg ggcacgttct
     2461 ccaccacggc gaagagcgtc gcggggtcat cagtgtctag gctctgtggc ttgtgggcgc
     2521 tgtcctccca cttctgaagg gggttgggga tggggtcgga ggcgagggct gtcgcaagga
     2581 tggggtcagc cccctgatag ggtggtgcca cctctctgcg gggagccgca aagttgggac
     2641 agtcaccggg ggtataggtg gagctggagg tgaaggtgga actgggcacg ggactgaagc
     2701 ccagggtggg ggtgaagcct ggagtgggac tgaagcttgg gtttggggcc aggggcttag
     2761 tagtagttcc ttcaagctcc ccctcttttt caggtgtcga tttcccacaa acaatggagt
     2821 agagcttgga cttccaccgt tggtagcgat acattaaacc aatgaagagg agggataaaa
     2881 ggcaaagacc aaagaaaatg accaggggca acagcactgt ggtgcctgag tcctcagtgc
     2941 ccttaacatt ctcaatctgg ggtaggcaca acttcgtgca ctccaggctt ttcttacagt
     3001 tactacagga gacacactcg ttttctctta gaaagaaacc tgcatggcag gtgcacacgg
     3061 tgttctgttt ctcctggcag gagaggtgca cggtcccatt gaggcagagg ctgcaattga
     3121 agcactggaa aaggttttca ctccaataat gccggtactg gttcttcctg cagccacaca
     3181 cggtgtcccg gtccactgtg caagaagaga tctccacctg acccatttcc tttcggcatt
     3241 tggagcagct gaggcagtgt ctgaggtggt tttctgaagc ggtgaaggag ccgctctcac
     3301 actccctgca gtccgtatcc tgccccgggc ctggacagtc attgtacaag taggttcctt
     3361 tgtggcactt ggtacagcaa atcgaattat tttgagggtg gatatatttt ccttggggac
     3421 acacactatc tctcttctcc ctgtccccta ggtgagggac cagtccaata acccctgagg
     3481 ggtatattcc caccaacagc tccaggagca ccagcggcag cagcaggtca ggcacggtgg
     3541 agaggcccat gccagacagc tatggcctct cactccccca tttgggctca gggcagtgtg
     3601 gcagcggcag tgctggggct tcccgggact cagcttgagc tttttgcaaa agcctaggcc
     3661 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     3721 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     3781 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     3841 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     3901 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     3961 taactgacac acattccaca gtcgggcagc gttgggtcct ggccacgggt gcgcatgatc
     4021 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
     4081 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
     4141 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca
     4201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4321 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4381 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4441 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4501 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4561 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4621 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4681 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4741 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4801 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4861 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4921 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4981 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5041 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5461 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5761 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6121 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6181 taggcgtatc acgaggccct ttcgtcttca agaa
//
