LOCUS       40924_41959        3987 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSVSPORTcHLhTNFE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3987)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3987)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        345..363
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             396..608
                     /codon_start=1
                     /note="unnamed protein product; chicken HL presequence"
                     /protein_id="SJL88333.1"
                     /translation="MDEERLSDNVRLYKGGSIRQGLRSFAAVYVLLALSFLLLTLLSSV
                     SLARIAALSSKLSTLQSEPKHNFSSR"
     CDS             609..1079
                     /codon_start=1
                     /note="unnamed protein product; human TNF mature sequence"
                     /protein_id="SJL88334.1"
                     /translation="VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRD
                     NQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRE
                     TPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL"
     misc_feature    1089..1127
                     /label=E-tag
                     /note="E-tag"
     promoter        complement(1305..1323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1412..1451
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     polyA_signal    1710..1844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2081..2659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2833..3690)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3691..3795)
                     /label=AmpR promoter
     promoter        3907..3938
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3946..3962
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat
      361 agaaggtacg cctgcaggta ccggtccgga attccatgga cgaggagagg ctcagcgaca
      421 atgtgaggct ctataaagga ggctccatcc ggcaggggct gcgatccttc gctgctgttt
      481 acgtcctcct ggcgctctcc ttcctcctcc tcaccctgct gagctccgtc tcattagcca
      541 gaattgctgc tctctcctcc aagctcagca ctctgcagtc ggagccgaag cacaacttct
      601 ccagccgggt cagatcatct tctcgaaccc cgagtgacaa gcctgtagcc catgttgtag
      661 caaaccctca agctgagggg cagctccagt ggctgaaccg ccgggccaat gccctcctgg
      721 ccaatggcgt ggagctgaga gataaccagc tggtggtgcc atcagagggc ctgtacctca
      781 tctactccca ggtcctcttc aagggccaag gctgcccctc cacccatgtg ctcctcaccc
      841 acaccatcag ccgcatcgcc gtctcctacc agaccaaggt caacctcctc tctgccatca
      901 agagcccctg ccagagggag accccagagg gggctgaggc caagccctgg tatgagccca
      961 tctatctggg aggggtcttc cagctggaga agggtgaccg actcagcgct gagatcaatc
     1021 ggcccgacta tctcgacttt gccgagtctg ggcaggtcta ctttgggatc attgccctgg
     1081 cggccgcagg tgcgccggtg ccgtatccgg atccgctgga accgcgtgcc gcatagactg
     1141 ttgaaagttg tttagcaaaa cctcatacag aaaattcatt tactaacgtc tggaaagacg
     1201 acaaaacttt agatcgttac gctaactatg agggctgtct gtggaatgtc gactctagag
     1261 gatccaagct tacgtacgcg tgcatgcgac gtcatagctc tctccctata gtgagtcgta
     1321 ttataagcta gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     1381 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     1441 gtgttaaact agctgcatat gcttgctgct tgagagtttt gcttactgag tatgatttat
     1501 gaaaatatta tacacaggag ctagtgattc taattgtttg tgtattttag attcacagtc
     1561 ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata
     1621 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1681 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     1741 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1801 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcctgcat taatgaatcg
     1861 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1921 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1981 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2041 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2101 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2161 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2221 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     2281 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2341 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2401 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2461 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2521 ggacagtatt tggtatctgc ggccagttac cttcggaaaa agagttggta gctcttgatc
     2581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2701 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2761 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2821 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2881 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2941 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3001 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3061 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3121 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3181 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3241 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3301 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3361 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3421 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3481 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3541 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3601 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3661 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3721 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3781 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     3841 tatcatgaca ttaacctata aaaataggcg tatacgaggc cctttcactc attaggcacc
     3901 ccaggcttta cactttatag cttccggctc gtataatgtg tggaattgtg agcggataac
     3961 aatttcacac aggaaacagc atcgatg
//
