LOCUS       .                      12795 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        7470..7827
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             7841..8362
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             8562..8582
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    10336..10470
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    10612..10752
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10938..11526)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11700..12557)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(12558..12662)
                     /label="AmpR promoter"
     promoter        12777..12795
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttaaaacagc ctgtgggttg ttcccacccg cagggcccac tgggcgctag cacactggta
       61 tcccggtacc cttgtgcgcc tgttttatat accctccccc ttatgtaact tagaagtatg
      121 attcaaacgg tcgacaggcg gctcagtgca ccaactgagt catgaccaag cacttctgtt
      181 accccggact gagtatcaat aagctgttca cacggctgaa ggagaaaacg ttcgttaccc
      241 ggccaattac ttcgagaaac ctagtaccac catgaaggtt gcgcagtgtt tcgctccaca
      301 caaccccagt gtagatcagg tcgatgagtc accgcattcc ccacgggcga ccgtggcggt
      361 ggctgcgttg gcggcctgcc catggggcaa cccatgggac gcttcaatac tgacatggtg
      421 tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcccaact
      481 gtggagcaga tactcacaaa ccagtgagcg gtctgtcgta acgggcaact ccgcagcgga
      541 accgactact ttgggtgtcc gtgtttcttt ttattcttac attggctgct tatggtgaca
      601 attgacaaat tgttaccata tagctattgg attggccatc cggtgacaaa cagagctatt
      661 gtttacttgt ttgttggttt cataccatta aattacaagg tcttagaaac tctcaacttt
      721 attttgacac tcaatacagc aaaatgggag cccaagtctc gacgcaaaaa tccggcagtc
      781 acgagactgg taacgtagcc actggaggat ctacaataaa ctttactaac atcaactatt
      841 ataaagattc ttacgccgcg tcagctaccc ggcaagactt cacacaggat ccaaagaaat
      901 tcacacaacc agtgttagat tctattagag aactatcagc ccctttgaac tccccttctg
      961 tggaagcttg tggctatagt gatagggtcg cccagctcac tgttgggaac tcctccatta
     1021 ctacccaaga ggctgctaat attgtactag cttatggaca gtggcctgag tactgccctg
     1081 acacagacgc aactgccgtg gataaaccaa ctcgcccgga cgtgtctgtc aacaggttct
     1141 acactttgga ctccaaaatg tggcaagaaa actcgactgg ttggtattgg aaattccctg
     1201 acgtgctgaa caagacgggg gtgttcggac agaatgccca gtttcattac ttgtaccggt
     1261 caggtttctg cttgcatgtg cagtgtaatg ctagtaaatt ccaccagggg gcccttcttg
     1321 tggctgtaat accagaattt gtaattgctg gcagaggatc taacacaaaa ccaaatgaag
     1381 cgccccaccc tgggtttacc acaactttcc ctggtaccac cggcgctaca tttcacgatc
     1441 catacgttct ggattccggg gtaccattga gtcaggctct gatatacccc catcaatgga
     1501 tcaatctccg cactaacaac tgtgcaactg ttatagttcc atacatcaat gctgttccgt
     1561 ttgactcagc catcaaccat agcaattttg ggctaatagt gataccagtt agtccgctga
     1621 agtattcgtc cggggcaacc actgcaatcc caatcactat cactatagcc cccctgaatt
     1681 cagagttcgg agggctgcga caagccgtca gtcaaggcat cccagctgag ctcaggcccg
     1741 ggactaatca gttcctgacc actgatgatg acactgcagc gcccatcctt ccaggattca
     1801 cccccacacc cacaattcat ataccagggg aagtacactc tttactggag ttgtgtaggg
     1861 tggagaccat tttggaagtg aacaacacca cagaatcaac aggattaaca aggctcttaa
     1921 taccagtgtc ctcgcagaac aaagccgatg agttgtgtgc tgcgtttatg gttgatccag
     1981 gccggattgg accttggcaa tctaccttag ttggacagat ttgccggtac tatacacaat
     2041 ggtccgggtc tttgaaggtt acctttatgt tcacaggatc cttcatggca acaggcaaga
     2101 tgctagtggc ctactcccca cccggaagcg ctcaaccagc caacagagaa accgccatgc
     2161 tgggcacgca cgtcatttgg gactttggac tacaatcgtc agtctctttg gtgataccgt
     2221 ggatcagtaa cactcacttc cgcactgcca agacaggtgg gaattacgat tactacacgg
     2281 caggtgtagt gaccttatgg tatcaaacca attacgtggt cccaccagaa acccctggag
     2341 aagcatatat tattgcaatg ggggcagcac aagacaactt cactttgaag atttgcaagg
     2401 atactgatga agtgacacaa caggctgtgt tgcaaggcga tcctgtggag gatataatcc
     2461 atgacgctct gggaaacaca gtgcgcagag ctattagcag tgctacaaat gttgaatccg
     2521 cagccaacac cactcccagt tcacaccgat tagagactgg acgcgtgcca gcgctacaag
     2581 ctgcagagac gggtgccact tctaatgcca cagatgagaa catgattgaa acccgttgtg
     2641 tggttaacag aaatggggta ttggaaacca ctatcaacca cttcttctcc cgctctggat
     2701 tagtgggagt agttaacctc acagatgggg ggacggacac tactgggtat gccacatggg
     2761 acatagatat tatgggcttt gtccaactcc gcagaaaatg cgagatgttc acatacatga
     2821 ggttcaacgc agaattcaca tttgtcacaa cgactaagaa tggggaggct cgtccgtaca
     2881 tgctgcaata tatgtatgtg ccccctggcg cccctaaacc gacgggaagg gatgcctttc
     2941 aatggcaaac agcaactaac ccatcagtct tcgtcaaact cactgaccct cctgcacaag
     3001 tctcagttcc tttcatgtca ccagctagtg catatcagtg gttttatgat ggttacccca
     3061 ctttcggcca gcacccggag acctcaaaca caacatatgg attgtgccca aacaatatga
     3121 tgggcacttt tgcagtgaga gttgtcagta gggaggcaag ccaactaaaa ctacagacca
     3181 gagtgtacat gaagcttaag catgtgaggg cctgggtccc aagaccgatc aggtctcaac
     3241 catacctgct taaaaacttc cccaattacg acagtagcaa gattgccaac agtgcacggg
     3301 accgatccag catcaagcaa gctaatatgg gtaaatttgg gcaacaatct ggcgccatct
     3361 atgttggtaa ttacagagtg gtcaatagac acctggccac ccacaatgac tgggccaacc
     3421 tggtgtggga agacagttct cgagacctgc ttgtttcgtc taccactgct cagggttgcg
     3481 atacgattgc ccgttgtgag tgtcagacag gggtgtacta ttgtaactca aggaggaaac
     3541 attatccagt cagcttctct aaacctagtc tcgtcttcgt agaggccagt gagtattacc
     3601 ctgctagata tcaatctcac ctgatgctcg ctgcaggcca ctctgaacct ggggattgcg
     3661 ggggtatctt gagatgtcag catggtgtgg ttggcatagt atccactggt ggcaacggtc
     3721 ttgtcggttt tgctaacgtg agggacctct tatggttgga tgaagaagcc atggaacagg
     3781 gagtgtctga ctatatcaag ggactcggcg atgcttttgg tattggtttt actgacgcag
     3841 tgtccaggga agtggaggct ctgaaaaatc acctgattgg ttccgaggga gcggtagaga
     3901 agatcctgaa gaacttagtg aaactcatat cagctctggt catagttatc agaagtgact
     3961 atgacatggt cacccttacc gcaactctag ctctgattgg gtgtcacggg agcccatggg
     4021 cgtggatcaa agcaaagacg gcgtccatct taggtattcc tatggcacag aagcagagtg
     4081 catcttggct taagaagttc aacgatatgg cgaacgctgc aaagggattg gagtggatct
     4141 ccaacaaaat cagcaaattt attgattggc tcaaggaaaa gatcattccg gccgcgaagg
     4201 aaaaagttga gtttcttaat aaccttaagc aactgccttt gatagaaaac caaattgcca
     4261 acttagaaca gtctgccgct tcacaagaag accttgaagt catgtttggt aatgtgtcat
     4321 acctagctca cttttgccgc aagttccagc cactctatgc aactgaagcg aagagagtgt
     4381 acgccttaga gaagagaatg aataattaca tgcagttcaa gagcaaacac cgtattgaac
     4441 ccgtatgttt aattatcagg ggctcaccag gaactggcaa gtcactcgcc acaggtataa
     4501 tagcgagagc cattgctgac aaatatcact ctagtgtgta ctcccttcca ccagacccag
     4561 atcactttga tggatacaag caacaggtgg tgacagtcat ggatgattta tgtcaaaacc
     4621 cagacggcaa agatatgtca ttgttctgtc aaatggtgtc cactgtcgat tttatacccc
     4681 caatggcttc actggaagag aaaggcgtat ccttcacatc taaatttgtt attgcttcaa
     4741 ctaatgctag caacatcatt gtccccacgg tctcagactc tgacgctatt cgtaggagat
     4801 ttttcatgga ttgtgatatc gaagtgactg actcttacaa gacagactta ggccgcttgg
     4861 atgcgggtag ggccgcaaag ctctgctcag agaacaacac tgccaatttc aagaggtgca
     4921 gcccgctagt gtgcggtaaa gccatccaac tgagagatag gaagtccaaa gtcaggtata
     4981 gcgtagacac tgtggtatca gaactggtta gggagtataa taacaggtcc gctataggaa
     5041 acactataga agctttattt caagggcctc ctaaatttag gcctataaga attagtcttg
     5101 atgagaaacc cgctccagat gctattagtg acctgcttgc tagtgttgac agtgaagagg
     5161 tgcggcagta ctgcagagat caaggatgga taattcctga agcgccaacc aacgtggagc
     5221 ggcatctcaa tagggcagtg ttagtaatgc aatccatcgc tactgtagtc gcagttgtat
     5281 cccttgttta cgttatctac aagctatttg ctggtttcca aggcgcatat tctggagcac
     5341 ccaagcagac tctcaagaaa cctgtgttga ggacggccac tgttcaaggg cccagcttag
     5401 attttgccct gtctctcctg cggcgtaaca tcaggcaggt gcaaaccgac caagggcact
     5461 ttaccatgct tggggtgcgt gaccgccttg ctatcttacc tcgtcactca caaccaggga
     5521 agaccatctg ggttgagcac aagttgatca atgtgcttga tgctgttgag ctagtggatg
     5581 aacaaggtgt taatttggag ctcacactag taaccctaga caccaatgaa aagtttagag
     5641 atgtcaccaa gttcattcca gagaatatca gtggagccag tgatgccaca ttagtaatca
     5701 acactgaaca catgccatca atgtttgtcc cggtaggaga tgttgttcaa tacgggttct
     5761 taaatcttag tggtaagcca acccacagga ccatgatgta caatttccct acaaaggctg
     5821 gacagtgtgg aggcgtggta acatctgttg gtaaaatcat tggcatccac attggaggca
     5881 atgggcgaca agggttctgc gctggcctga agaggagtta ttttgcaagt gaacaaggtg
     5941 agatccaatg ggtgaaacct aacaaggaga ccggcagatt aaatatcaat ggcccaacac
     6001 gcaccaagtt agagcccagt gtgttccatg atgtgtttga gggcaataag gagccagcag
     6061 tcctaacgag caaggatccc agattagagg ttgactttga gcaagccctc ttctccaagt
     6121 atgtgggcaa tgtcctccac gaacctgacg aatacgtaaa gcaggcagcc ctccactacg
     6181 caaaccagct caagcaactg gatataaaca ccaataggat gagcatggag gaagcgtgct
     6241 atggcacaga gaacctggaa gcaattgacc tccacactag cgcagggtat ccatacagtg
     6301 ctctaggcat aaagaagagg gacatactag atcctaccac tagggacaca acaaaaatga
     6361 agttctacat ggacaagtat ggcttggact taccttactc cacctacgtt aaagatgaac
     6421 ttaggtctct ggataagatc aagaaaggga agtcccgctt gatagaagct agcagcctga
     6481 atgactcagt gtacctcaga atgacttttg gccacctgta tgaagcattc catgcaaatc
     6541 cagggactgt gaccggatca gcagtcgggt gcaatccgga tgtgttctgg agtaaactcc
     6601 caatcctgct cccaggctcg ctatttgcct ttgactattc aggctatgat gctagtctta
     6661 gccccgtctg gttcagggct ttggagatgg tcttacggga cattggctac tcagaggaag
     6721 cagtgtcact catagaagga ataaatcata cccatcatgt gtatcggaac aaaacatatt
     6781 gtatccttgg tgggatgccg tcaggatgtt ctggcacctc cattttcaac tcgatgatca
     6841 ataacatcat catcaggacg cttttaatca aaacctttaa agggatagat ctagacgagt
     6901 tgaatatggt ggcctacgga gatgatgtgc tagctagtta tcctttcccc attgattgcc
     6961 ttgagctagc taagactggc aaagaatatg ggctgaccat gacacccgca gacaaatcac
     7021 cttgcttcaa tgaagtgacg tgggaaaacg ccacctttct gaagagaggg tttctaccag
     7081 accatcaatt cccattcttg attcatccta taatgcccat gaaagagatc cacgagtcca
     7141 tacgctggac caaagatgcg tgcaataccc aagaccatgt gcgctctctg tgtttattag
     7201 cttggcacaa tggtaaggat gaatatgaaa aatttgtgag tacaattaga tcagtcccag
     7261 ttgggaaagc attagcaatt ccgaattttg agaatttgag aagaaattgg ctcgaactat
     7321 tttagattta cagttgaaag ctgaacccca ccagaaatct ggtcgtgtta atgactggtg
     7381 ggggtaaatt tgttataacc agaatagcaa aaaaaaaaaa aaaaagtcga caattctcat
     7441 gtttgacagc ttatcatcga taagctcttc tgaggcggaa agaaccagct gtggaatgtg
     7501 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7561 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7621 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7681 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7741 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7801 ttttttggag gcctaggctt ttgcaaaaag ctttgcaaag atggataaag ttttaaacag
     7861 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct gggggaatat
     7921 tcctctgatg agaaaggcat atttaaaaaa atgcaaggag tttcatcctg ataaaggagg
     7981 agatgaagaa aaaatgaaga aaatgaatac tctgtacaag aaaatggaag atggagtaaa
     8041 atatgctcat caacctgact ttggaggctt ctgggatgca actgaggtat ttgcttcttc
     8101 cttaaatcct ggtgttgatg caatgtactg caaacaatgg cctgagtgtg caaagaaaat
     8161 gtctgctaac tgcatatgct tgctgtgctt actgaggatg aagcatgaaa atagaaaatt
     8221 atacaggaaa gatccacttg tgtgggttga ttgctactgc ttcgattgct ttagaatgtg
     8281 gtttggactt gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     8341 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     8401 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     8461 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     8521 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     8581 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     8641 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     8701 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     8761 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     8821 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     8881 tgtatagtgc cttgactaga gatccatttt ctgttattga ggaaagtttg ccaggtgggt
     8941 taaaggagca tgattttaat ccagaagaag cagaggaaac taaacaagtg tcctggaagc
     9001 ttgtaacaga gtatgcaatg gaaacaaaat gtgatgatgt gttgttattg cttgggatgt
     9061 acttggaatt tcagtacagt tttgaaatgt gtttaaaatg tattaaaaaa gaacagccca
     9121 gccactataa gtaccatgaa aagcattatg caaatgctgc tatatttgct gacagcaaaa
     9181 accaaaaaac catatgccaa caggctgttg atactgtttt agctaaaaag cgggttgata
     9241 gcctacaatt aactagagaa caaatgttaa caaacagatt taatgatctt ttggatagga
     9301 tggatataat gtttggttct acaggctctg ctgacataga agaatggatg gctggagttg
     9361 cttggctaca ctgtttgttg cccaaaatgg attcagtggt gtatgacttt ttaaaatgca
     9421 tggtgtacaa cattcctaaa aaaagatact ggctgtttaa aggaccaatt gatagtggta
     9481 aaactacatt agcagctgct ttgcttgaat tatgtggggg gaaagcttta aatgttaatt
     9541 tgcccttgga caggctgaac tttgagctag gagtagctat tgaccagttt ttagtagttt
     9601 ttgaggatgt aaagggcact ggaggggagt ccagagattt gccttcaggt cagggaatta
     9661 ataacctgga caatttaagg gattatttgg atggcagtgt taaggtaaac ttagaaaaga
     9721 aacacctaaa taaaagaact caaatatttc cccctggaat agtcaccatg aatgagtaca
     9781 gtgtgcctaa aacactgcag gccagatttg taaaacaaat agattttagg cccaaagatt
     9841 atttaaagca ttgcctggaa cgcagtgagt ttttgttaga aaagagaata attcaaagtg
     9901 gcattgcttt gcttcttatg ttaatttggt acagacctgt ggctgagttt gctcaaagta
     9961 ttcagagcag aattgtggag tggaaagaga gattggacaa agagtttagt ttgtcagtgt
    10021 atcaaaaaat gaagtttaat gtggctatgg gaattggagt tttagattgg ctaagaaaca
    10081 gtgatgatga tgatgaagac agccaggaaa atgctgataa aaatgaagat ggtggggaga
    10141 agaacatgga agactcaggg catgaaacag gcattgattc acagtcccaa ggctcatttc
    10201 aggcccctca gtcctcacag tctgttcatg atcataatca gccataccac atttgtagag
    10261 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    10321 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10441 ctcatcaatg tatcttatca tgtctggatc ctgcctcgcg cgtttcggtg atgacggtga
    10501 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10561 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    10621 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    10681 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10741 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    11041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    11101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    11161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    11341 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    11401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11581 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    11641 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11701 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11761 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11821 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11881 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11941 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    12001 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    12061 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    12121 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    12181 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    12241 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    12301 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    12361 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    12421 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    12481 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    12541 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    12601 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    12661 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    12721 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattgcg gccgcgtaat
    12781 acgactcact atagg
//
