LOCUS       V002925                11096 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002925
VERSION     V002925
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11096)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 11096)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11096)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(4011..5468)
                     /gene="P4hb"
                     /label="Protein disulfide-isomerase"
                     /note="Protein disulfide-isomerase from Rattus norvegicus.
                     Accession#: P04785"
     misc_feature    complement(5478..5534)
                     /label="srPDI"
                     /note="srPDI"
     misc_feature    complement(5605..6542)
                     /label="intron"
                     /note="intron"
     CDS             complement(6549..6734)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        6806..7135
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             7149..7670
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             7870..7890
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    8945..9085
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9271..9859)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10204..10860)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(10861..10963)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagggggnnn ggatccnnng aattcnnnat
     3121 ggtagtaaaa tttcaacttt atttagccaa tgtgttcaat tccaacatta tggtttagaa
     3181 atagaccttg agaatgggca tttcattcag ccccagtgtc cttagctgac tcccagtctg
     3241 ggaatcaagg gtcccggttc tgcctcacac aggccacatg ccatgctgga agaggatcca
     3301 gcagccagag taatgtgccc acagccggcc ggctggtcgg tagttctggc ttaagaagag
     3361 tgagagctgt ccaggcctgg cctgagcctg gcagcaagcc accactgtct cttttccaca
     3421 cgtggggacc caccctctga caggatgcag aaatccccag gttaaatgca aactacaaaa
     3481 gccatgctgt catggcaaca ctagtacaag gtggagagag agagagcata cgacccagaa
     3541 ccatcgcctc ctgcgcaggg caggaagggg tctcagggag gtctgcgtgg tggaacaagt
     3601 acctcgacat ggagacatgt caggaagatg aaacatctca cagacgaaat ctgaagtgat
     3661 agcataaacc tctctaacag gaaagacatt accagtgtga aatccccaaa tatggtagca
     3721 agcacttggg acaaaatcca cgtacaaaaa agtacaccac caattaaaac aaaacgtagc
     3781 ccttcaagat gggctggcct ggacagagat ccctttccaa aattgaaaag caaaaaaaga
     3841 agagacaggt ttaagtgtac gtgtgtcaga tgagtgtggc ttcagaaaga ttccctgggt
     3901 tctgatggcg ctccctcctg gggtgaggct caggctccgg agatgtgggc tgctgggaca
     3961 tggcccaccg aggttttggg ttcaggcgcc cagatctggc ttctgcacta cagttcatcc
     4021 ttcacggctt tctgatcgtc gtcttcttcc atatctggct ctaaagcttc ttctaggtcg
     4081 aggtcgtcat tgtcccccgc tccatcctgg ccaccgctct ccaagaattt cttaaaacca
     4141 tctagtgtcc gctcaccgtt gtaatcaatg accgttctgt ctgcacttgc tgggaagaac
     4201 ttgagtgtgg gaaagctgtg caccttcaca gcttccacct cattggctgt tgagtccatc
     4261 ttagcgatga cgatattctc atggtctttg tatgtctctc ccagtttatc ccaaatcggg
     4321 gctagctgct tgcagtgacc acaccaggga gcatagaatt caacaaacac gttctttttc
     4381 tcatcaaaag caacctcctc aaagtttttc ccaactagca ctttcactgg ctgcttgtcc
     4441 cagtcttcag gcagttcctg gctcatcagg tggggcttga tcttgccctc caggaagtgg
     4501 tggcaaaatt gtgtgatctt ctcagctgtc agctcgtctg actccggttt gtacttggtc
     4561 atctcttcct ccagggtaat aagccgcaca gctggacatt cctccttctt caggccaaag
     4621 aactcaagta tgcgctggtt gtcagtgtgg tcactatcga tgaagatgaa caggatcttg
     4681 cccttaaagc cctcggccgc tttcttaaag ttgctcaatt tgccatcgta gtcagacaca
     4741 ctcttgggca ggaacagcag aatatgtgtc ttgatttcac ctccgaaaat ctttggagct
     4801 gtctgttcag tgaactcgat gaccaaaggc agctggttgt gcttgatgaa gtctaatagc
     4861 ttctccttgg tgatctcacc ttcaaaattg ttgcggcctt catcaaactt cttaaagagg
     4921 accaccccat ccttgtccag ctggtacttg gaaaacacat cgctattgga agtgattcca
     4981 aaaggtatgt catcaacagc ctctgctgcc agcaagaact gcttggcgga gtctgaccct
     5041 gcgtccttga agaagccgat gaccgtcact tcgcttgagt ccaccaagga ctctgcagct
     5101 gcagtgtcag acagggttgt ggctgctggg cctgtgcgtt tcttcagcca gttcacaatg
     5161 tcgtcagctt ccctgccagc tgtatattcc tttggggagg ctgtgtctcc attcttgaag
     5221 aacttgattg tggggtagcc acggacacca tactgctggg ccaggtcaga ctcttctgtg
     5281 gcgtccacct ttgctagtcg gatctcagag ccttctgcct tcagttttgc agcagctttg
     5341 gcatactctg gggccagtgc tttgcagtgg ccacaccatg gggcatagaa ctccaccagc
     5401 aggtagttgt gcgccgccgg ctctgcgaag ttgctcttct tcagcaccag gacgttgtcc
     5461 tcctcctcca gagcgtcggc gcccacccta gccgcccagg ccagggccag gcacagcaaa
     5521 gcacggctca gcatgtcggc ctctagaggc tgcagccgac cccggatccg gagcttttag
     5581 agcagaagta acacttccgt acaggcctag aagtaaaggc aacatccact gaggagcagt
     5641 tctttgattt gcaccaccag gagcctcaaa tttttcaata aattcacctg actgcacatt
     5701 aggactttgg ctttgagctt cccacctctc agttacttgc tgaatactgt ctgcttcatc
     5761 aatattatca taggtgtgcc caaatgatat ttgcaaccct tccctgttgg ctacttgtct
     5821 caccattgta ggcctaatgg gagacaaagt agagtagtaa tcttgtaaag agttatacca
     5881 attaacagga gcattaatta ctgtccaagt agtttcctct aaaaaccttg ccaaactgtc
     5941 ccttaaatat ctttgggttc ttctttcaag ctcctgtgag gtgagcctag gaatgtcatt
     6001 ttgtattaca cgccaaaaag cttgagaaat ggcattaaaa agtgttggac cccaatgtct
     6061 ggggtcaaga tactgaacac tgtgaacaaa ggtttgtact ccaggaaata aaatatcata
     6121 gtaatcatct ggcctataca aatctacagc cattcctggt tgttgatata aaccaacagt
     6181 agaaactttg tgatcccagt cactaaaaaa tctatacccc acttgagcaa cagcgctcac
     6241 accagtcaca gtttgcagta aagctgcaaa tccagctata gcagcaggag ccccagatat
     6301 cacagcatag gcctgtggag tgaggcctat agcagcaatt gcctcagagg ttgttaggcc
     6361 ttcaacagta gcaacagatg caagttgcac ttcaattgca gcagcggcct ctccagcagc
     6421 aatttcagct actgaaaatc cagtagcagc agcagcttca gacacagtag caattaggtc
     6481 ccccaacagt gttaaagcag cacccatgga cctgaaataa aagacaaaaa gactaaactt
     6541 accagttaac tttctggttt ttcagttaac ctttctggtt ttttgcgttt cccgtcaaca
     6601 gtatcttccc cttcacaaaa ttgcagcaaa agctctaaaa caaacacaaa aaggcgttga
     6661 gctgtttttt tactttcagt ccatgaccta cgaaccttaa cggaggcctg gcgtgacagc
     6721 cggcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     6781 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7141 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     7201 gtcttgaaag gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat
     7261 gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc
     7321 tgtacaagaa aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct
     7381 gggatgcaac tgaggtattt gcttcttcct taaatcctgg tgttgatgca atgtactgca
     7441 aacaatggcc tgagtgtgca aagaaaatgt ctgctaactg catatgcttg ctgtgcttac
     7501 tgaggatgaa gcatgaaaat agaaaattat acaggaaaga tccacttgtg tgggttgatt
     7561 gctactgctt cgattgcttt agaatgtggt ttggacttga tctttgtgaa ggaaccttac
     7621 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     7681 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     7741 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     7801 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     7861 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     7921 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     7981 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     8041 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     8101 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     8161 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tccattttct
     8221 gttattgagg aaagtttgcc aggtgggtta aaggagcatg attttaatcc agaagaagca
     8281 gaggaaacta aacaagtgtc ctggaagctt gtaacagagt atgcaatgga aacaaaatgt
     8341 gatgatgtgt tgttattgct tgggatgtac ttggaatttc agtacagttt tgaaatgtgt
     8401 ttaaaatgta ttaaaaaaga acagcccagc cactataagt accatgaaaa gcattatgca
     8461 aatgctgcta tatttgctga cagcaaaaac caaaaaacca tatgccaaca ggctgttgat
     8521 actgttttag ctaaaaagcg ggttgatagc ctacaattaa ctagagaaca aatgttaaca
     8581 aacagattta atgatctttt ggataggatg gatataatgt ttggttctac aggctctgct
     8641 gacatagaag aatggatggc tggagttgct tggctacact gtttgttgcc caaaatggat
     8701 tcagtggtgt atgacttttt aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg
     8761 ctgtttaaag gaccaattga tagtggtaaa actacattag cagctgcctc gcgcgtttcg
     8821 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     8881 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     8941 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     9001 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9061 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9901 gaacgaaaac tcacgttaag ggattttggt catgatgata tatttttatc ttgtgcaatg
     9961 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
    10021 ttgactcccc gcgcgcgatg ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc
    10081 gggctaaaaa aaagcccgct cattaggcgg gctcgaattt ctgccattca tccgcttatt
    10141 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
    10201 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
    10261 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
    10321 gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattcgc
    10381 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
    10441 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
    10501 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
    10561 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
    10621 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
    10681 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
    10741 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
    10801 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
    10861 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
    10921 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
    10981 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
    11041 gtgatcttcc gtcacaggta tttattcgaa gacgaaaggg catcgcgcgc ggggaa
//
