LOCUS       V002926                11146 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002926
VERSION     V002926
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11146)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 11146)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11146)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(4011..5468)
                     /gene="P4hb"
                     /label="Protein disulfide-isomerase"
                     /note="Protein disulfide-isomerase from Rattus norvegicus.
                     Accession#: P04785"
     misc_feature    complement(5478..5534)
                     /label="srPDI"
                     /note="srPDI"
     gap             5579..5588
                     /estimated_length=10
     misc_feature    complement(5655..6592)
                     /label="intron"
                     /note="intron"
     CDS             complement(6599..6784)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        6856..7185
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             7199..7720
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             7920..7940
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    8995..9135
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9321..9909)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10254..10910)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(10911..11013)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagggggnnn ggatccnnng aattcnnnat
     3121 ggtagtaaaa tttcaacttt atttagccaa tgtgttcaat tccaacatta tggtttagaa
     3181 atagaccttg agaatgggca tttcattcag ccccagtgtc cttagctgac tcccagtctg
     3241 ggaatcaagg gtcccggttc tgcctcacac aggccacatg ccatgctgga agaggatcca
     3301 gcagccagag taatgtgccc acagccggcc ggctggtcgg tagttctggc ttaagaagag
     3361 tgagagctgt ccaggcctgg cctgagcctg gcagcaagcc accactgtct cttttccaca
     3421 cgtggggacc caccctctga caggatgcag aaatccccag gttaaatgca aactacaaaa
     3481 gccatgctgt catggcaaca ctagtacaag gtggagagag agagagcata cgacccagaa
     3541 ccatcgcctc ctgcgcaggg caggaagggg tctcagggag gtctgcgtgg tggaacaagt
     3601 acctcgacat ggagacatgt caggaagatg aaacatctca cagacgaaat ctgaagtgat
     3661 agcataaacc tctctaacag gaaagacatt accagtgtga aatccccaaa tatggtagca
     3721 agcacttggg acaaaatcca cgtacaaaaa agtacaccac caattaaaac aaaacgtagc
     3781 ccttcaagat gggctggcct ggacagagat ccctttccaa aattgaaaag caaaaaaaga
     3841 agagacaggt ttaagtgtac gtgtgtcaga tgagtgtggc ttcagaaaga ttccctgggt
     3901 tctgatggcg ctccctcctg gggtgaggct caggctccgg agatgtgggc tgctgggaca
     3961 tggcccaccg aggttttggg ttcaggcgcc cagatctggc ttctgcacta cagttcatcc
     4021 ttcacggctt tctgatcgtc gtcttcttcc atatctggct ctaaagcttc ttctaggtcg
     4081 aggtcgtcat tgtcccccgc tccatcctgg ccaccgctct ccaagaattt cttaaaacca
     4141 tctagtgtcc gctcaccgtt gtaatcaatg accgttctgt ctgcacttgc tgggaagaac
     4201 ttgagtgtgg gaaagctgtg caccttcaca gcttccacct cattggctgt tgagtccatc
     4261 ttagcgatga cgatattctc atggtctttg tatgtctctc ccagtttatc ccaaatcggg
     4321 gctagctgct tgcagtgacc acaccaggga gcatagaatt caacaaacac gttctttttc
     4381 tcatcaaaag caacctcctc aaagtttttc ccaactagca ctttcactgg ctgcttgtcc
     4441 cagtcttcag gcagttcctg gctcatcagg tggggcttga tcttgccctc caggaagtgg
     4501 tggcaaaatt gtgtgatctt ctcagctgtc agctcgtctg actccggttt gtacttggtc
     4561 atctcttcct ccagggtaat aagccgcaca gctggacatt cctccttctt caggccaaag
     4621 aactcaagta tgcgctggtt gtcagtgtgg tcactatcga tgaagatgaa caggatcttg
     4681 cccttaaagc cctcggccgc tttcttaaag ttgctcaatt tgccatcgta gtcagacaca
     4741 ctcttgggca ggaacagcag aatatgtgtc ttgatttcac ctccgaaaat ctttggagct
     4801 gtctgttcag tgaactcgat gaccaaaggc agctggttgt gcttgatgaa gtctaatagc
     4861 ttctccttgg tgatctcacc ttcaaaattg ttgcggcctt catcaaactt cttaaagagg
     4921 accaccccat ccttgtccag ctggtacttg gaaaacacat cgctattgga agtgattcca
     4981 aaaggtatgt catcaacagc ctctgctgcc agcaagaact gcttggcgga gtctgaccct
     5041 gcgtccttga agaagccgat gaccgtcact tcgcttgagt ccaccaagga ctctgcagct
     5101 gcagtgtcag acagggttgt ggctgctggg cctgtgcgtt tcttcagcca gttcacaatg
     5161 tcgtcagctt ccctgccagc tgtatattcc tttggggagg ctgtgtctcc attcttgaag
     5221 aacttgattg tggggtagcc acggacacca tactgctggg ccaggtcaga ctcttctgtg
     5281 gcgtccacct ttgctagtcg gatctcagag ccttctgcct tcagttttgc agcagctttg
     5341 gcatactctg gggccagtgc tttgcagtgg ccacaccatg gggcatagaa ctccaccagc
     5401 aggtagttgt gcgccgccgg ctctgcgaag ttgctcttct tcagcaccag gacgttgtcc
     5461 tcctcctcca gagcgtcggc gcccacccta gccgcccagg ccagggccag gcacagcaaa
     5521 gcacggctca gcatgtcgga cgtcggcagc gggcggggca gctaggttgg cgccggggnn
     5581 nnnnnnnngc ctctagaggc tgcagccgac cccggatccg gagcttttag agcagaagta
     5641 acacttccgt acaggcctag aagtaaaggc aacatccact gaggagcagt tctttgattt
     5701 gcaccaccag gagcctcaaa tttttcaata aattcacctg actgcacatt aggactttgg
     5761 ctttgagctt cccacctctc agttacttgc tgaatactgt ctgcttcatc aatattatca
     5821 taggtgtgcc caaatgatat ttgcaaccct tccctgttgg ctacttgtct caccattgta
     5881 ggcctaatgg gagacaaagt agagtagtaa tcttgtaaag agttatacca attaacagga
     5941 gcattaatta ctgtccaagt agtttcctct aaaaaccttg ccaaactgtc ccttaaatat
     6001 ctttgggttc ttctttcaag ctcctgtgag gtgagcctag gaatgtcatt ttgtattaca
     6061 cgccaaaaag cttgagaaat ggcattaaaa agtgttggac cccaatgtct ggggtcaaga
     6121 tactgaacac tgtgaacaaa ggtttgtact ccaggaaata aaatatcata gtaatcatct
     6181 ggcctataca aatctacagc cattcctggt tgttgatata aaccaacagt agaaactttg
     6241 tgatcccagt cactaaaaaa tctatacccc acttgagcaa cagcgctcac accagtcaca
     6301 gtttgcagta aagctgcaaa tccagctata gcagcaggag ccccagatat cacagcatag
     6361 gcctgtggag tgaggcctat agcagcaatt gcctcagagg ttgttaggcc ttcaacagta
     6421 gcaacagatg caagttgcac ttcaattgca gcagcggcct ctccagcagc aatttcagct
     6481 actgaaaatc cagtagcagc agcagcttca gacacagtag caattaggtc ccccaacagt
     6541 gttaaagcag cacccatgga cctgaaataa aagacaaaaa gactaaactt accagttaac
     6601 tttctggttt ttcagttaac ctttctggtt ttttgcgttt cccgtcaaca gtatcttccc
     6661 cttcacaaaa ttgcagcaaa agctctaaaa caaacacaaa aaggcgttga gctgtttttt
     6721 tactttcagt ccatgaccta cgaaccttaa cggaggcctg gcgtgacagc cggcgcagca
     6781 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     6841 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6901 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6961 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7021 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7081 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7141 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat
     7201 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     7261 gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt
     7321 tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa
     7381 aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct gggatgcaac
     7441 tgaggtattt gcttcttcct taaatcctgg tgttgatgca atgtactgca aacaatggcc
     7501 tgagtgtgca aagaaaatgt ctgctaactg catatgcttg ctgtgcttac tgaggatgaa
     7561 gcatgaaaat agaaaattat acaggaaaga tccacttgtg tgggttgatt gctactgctt
     7621 cgattgcttt agaatgtggt ttggacttga tctttgtgaa ggaaccttac ttctgtggtg
     7681 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     7741 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     7801 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     7861 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     7921 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     7981 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     8041 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     8101 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     8161 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     8221 ttaataagga atatttgatg tatagtgcct tgactagaga tccattttct gttattgagg
     8281 aaagtttgcc aggtgggtta aaggagcatg attttaatcc agaagaagca gaggaaacta
     8341 aacaagtgtc ctggaagctt gtaacagagt atgcaatgga aacaaaatgt gatgatgtgt
     8401 tgttattgct tgggatgtac ttggaatttc agtacagttt tgaaatgtgt ttaaaatgta
     8461 ttaaaaaaga acagcccagc cactataagt accatgaaaa gcattatgca aatgctgcta
     8521 tatttgctga cagcaaaaac caaaaaacca tatgccaaca ggctgttgat actgttttag
     8581 ctaaaaagcg ggttgatagc ctacaattaa ctagagaaca aatgttaaca aacagattta
     8641 atgatctttt ggataggatg gatataatgt ttggttctac aggctctgct gacatagaag
     8701 aatggatggc tggagttgct tggctacact gtttgttgcc caaaatggat tcagtggtgt
     8761 atgacttttt aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg ctgtttaaag
     8821 gaccaattga tagtggtaaa actacattag cagctgcctc gcgcgtttcg gtgatgacgg
     8881 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     8941 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     9001 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     9061 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     9121 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9181 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9241 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9301 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9361 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9421 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9601 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9661 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9721 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9781 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9841 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9901 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9961 tcacgttaag ggattttggt catgatgata tatttttatc ttgtgcaatg taacatcaga
    10021 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc
    10081 gcgcgcgatg ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa
    10141 aaagcccgct cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt
    10201 caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
    10261 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
    10321 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
    10381 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa
    10441 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
    10501 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
    10561 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
    10621 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
    10681 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
    10741 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
    10801 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
    10861 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
    10921 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
    10981 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
    11041 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
    11101 gtcacaggta tttattcgaa gacgaaaggg catcgcgcgc ggggaa
//
