LOCUS       V002934                10062 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002934
VERSION     V002934
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10062)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3219..3257)
                     /label="E-tag"
                     /note="E-tag"
     CDS             complement(3267..3584)
                     /codon_start=1
                     /note="unnamed protein product; CH3"
                     /protein_id="SJL88029.1"
                     /translation="QPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQ
                     PENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSLSP
                     GK"
     CDS             complement(3600..4457)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(4571..5508)
                     /label="intron"
                     /note="intron"
     CDS             complement(5515..5700)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5772..6101
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6115..6636
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6836..6856
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7911..8051
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8237..8825)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9170..9826)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9827..9929)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagtcgacat tccacagaca gccctcatag
     3121 ttagcgtaac gatctaaagt tttgtcgtct ttccagacgt tagtaaatga attttctgta
     3181 tgaggttttg ctaaacaact ttcaacagtc tatgcggcac gcggttccag cggatccgga
     3241 tacggcaccg gcgcacctgc ggccgcttta cccggagaca gggagaggct cttctgcgtg
     3301 aagcggttgt gcagagcctc atgcatcacg gagcatgaga agatgttccc ctgctgccac
     3361 ctgctcttgt ccacggtgag cttgctgtag aggaagaagg agccgtcgga gtccagcatg
     3421 ggaggcgtgg tgttgtagtt gttctccggc tgcccattgc tctcccactc cacggcgatg
     3481 tcgctggggt agaagccttt gaccaggcag gtcaggctga cttggttctt ggtcatctcc
     3541 tcccgggatg ggggcagggt gtacacctgt ggttctcggg gctggatccc cgggaattcc
     3601 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3661 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3721 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3781 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3841 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3901 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3961 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4021 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4081 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4141 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4201 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4261 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4321 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4381 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4441 aaatgttgaa tactcatact cttccttttt caatattatt gaagcagaca gttttattgt
     4501 tcatgcctct agaggctgca gccgaccccg gatccggagc ttttagagca gaagtaacac
     4561 ttccgtacag gcctagaagt aaaggcaaca tccactgagg agcagttctt tgatttgcac
     4621 caccaggagc ctcaaatttt tcaataaatt cacctgactg cacattagga ctttggcttt
     4681 gagcttccca cctctcagtt acttgctgaa tactgtctgc ttcatcaata ttatcatagg
     4741 tgtgcccaaa tgatatttgc aacccttccc tgttggctac ttgtctcacc attgtaggcc
     4801 taatgggaga caaagtagag tagtaatctt gtaaagagtt ataccaatta acaggagcat
     4861 taattactgt ccaagtagtt tcctctaaaa accttgccaa actgtccctt aaatatcttt
     4921 gggttcttct ttcaagctcc tgtgaggtga gcctaggaat gtcattttgt attacacgcc
     4981 aaaaagcttg agaaatggca ttaaaaagtg ttggacccca atgtctgggg tcaagatact
     5041 gaacactgtg aacaaaggtt tgtactccag gaaataaaat atcatagtaa tcatctggcc
     5101 tatacaaatc tacagccatt cctggttgtt gatataaacc aacagtagaa actttgtgat
     5161 cccagtcact aaaaaatcta taccccactt gagcaacagc gctcacacca gtcacagttt
     5221 gcagtaaagc tgcaaatcca gctatagcag caggagcccc agatatcaca gcataggcct
     5281 gtggagtgag gcctatagca gcaattgcct cagaggttgt taggccttca acagtagcaa
     5341 cagatgcaag ttgcacttca attgcagcag cggcctctcc agcagcaatt tcagctactg
     5401 aaaatccagt agcagcagca gcttcagaca cagtagcaat taggtccccc aacagtgtta
     5461 aagcagcacc catggacctg aaataaaaga caaaaagact aaacttacca gttaactttc
     5521 tggtttttca gttaaccttt ctggtttttt gcgtttcccg tcaacagtat cttccccttc
     5581 acaaaattgc agcaaaagct ctaaaacaaa cacaaaaagg cgttgagctg tttttttact
     5641 ttcagtccat gacctacgaa ccttaacgga ggcctggcgt gacagccggc gcagcaccat
     5701 ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc
     5761 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5821 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5881 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5941 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6001 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6061 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc aaagatggat
     6121 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     6181 gcctggggga atattcctct gatgagaaag gcatatttaa aaaaatgcaa ggagtttcat
     6241 cctgataaag gaggagatga agaaaaaatg aagaaaatga atactctgta caagaaaatg
     6301 gaagatggag taaaatatgc tcatcaacct gactttggag gcttctggga tgcaactgag
     6361 gtatttgctt cttccttaaa tcctggtgtt gatgcaatgt actgcaaaca atggcctgag
     6421 tgtgcaaaga aaatgtctgc taactgcata tgcttgctgt gcttactgag gatgaagcat
     6481 gaaaatagaa aattatacag gaaagatcca cttgtgtggg ttgattgcta ctgcttcgat
     6541 tgctttagaa tgtggtttgg acttgatctt tgtgaaggaa ccttacttct gtggtgtgac
     6601 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     6661 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     6721 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     6781 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     6841 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     6901 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     6961 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     7021 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     7081 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     7141 taaggaatat ttgatgtata gtgccttgac tagagatcca ttttctgtta ttgaggaaag
     7201 tttgccaggt gggttaaagg agcatgattt taatccagaa gaagcagagg aaactaaaca
     7261 agtgtcctgg aagcttgtaa cagagtatgc aatggaaaca aaatgtgatg atgtgttgtt
     7321 attgcttggg atgtacttgg aatttcagta cagttttgaa atgtgtttaa aatgtattaa
     7381 aaaagaacag cccagccact ataagtacca tgaaaagcat tatgcaaatg ctgctatatt
     7441 tgctgacagc aaaaaccaaa aaaccatatg ccaacaggct gttgatactg ttttagctaa
     7501 aaagcgggtt gatagcctac aattaactag agaacaaatg ttaacaaaca gatttaatga
     7561 tcttttggat aggatggata taatgtttgg ttctacaggc tctgctgaca tagaagaatg
     7621 gatggctgga gttgcttggc tacactgttt gttgcccaaa atggattcag tggtgtatga
     7681 ctttttaaaa tgcatggtgt acaacattcc taaaaaaaga tactggctgt ttaaaggacc
     7741 aattgatagt ggtaaaacta cattagcagc tgcctcgcgc gtttcggtga tgacggtgaa
     7801 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7861 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7921 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     7981 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     8041 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8101 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8161 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8401 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8641 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8761 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8821 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8881 gttaagggat tttggtcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     8941 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga ctccccgcgc
     9001 gcgatgggtc gaatttgctt tcgaaaaaaa agcccgctca ttaggcgggc taaaaaaaag
     9061 cccgctcatt aggcgggctc gaatttctgc cattcatccg cttattatca cttattcagg
     9121 cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     9181 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     9241 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     9301 atttgcccat agtgaaaacg ggggcgaaga agttgtccat attcgccacg tttaaatcaa
     9361 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     9421 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     9481 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     9541 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     9601 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     9661 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     9721 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     9781 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     9841 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     9901 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     9961 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
    10021 caggtattta ttcgaagacg aaagggcatc gcgcgcgggg aa
//
