LOCUS       V002935                10011 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002935
VERSION     V002935
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10011)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3096..3212)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(3213..3533)
                     /codon_start=1
                     /note="unnamed protein product; CH3"
                     /protein_id="SJL88034.1"
                     /translation="QPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQ
                     PENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSLSP
                     GK"
     CDS             complement(3549..4406)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(4520..5457)
                     /label="intron"
                     /note="intron"
     CDS             complement(5464..5649)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5721..6050
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6064..6585
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6785..6805
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7860..8000
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8186..8774)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9119..9775)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9776..9878)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagtcgacct gggtgcttta tttccatgct
     3121 gggtgcccgg gaagtatgta cacggggtac gtgccaagca tcctcgcgcg accccgagag
     3181 cccggggagc gggggcttgc cggctgtcgc actcatttac ccggagacag ggagaggctc
     3241 ttctgcgtga agcggttgtg cagagcctca tgcatcacgg agcatgagaa gatgttcccc
     3301 tgctgccacc tgctcttgtc cacggtgagc ttgctgtaga ggaagaagga gccgtcggag
     3361 tccagcatgg gaggcgtggt gttgtagttg ttctccggct gcccattgct ctcccactcc
     3421 acggcgatgt cgctggggta gaagcctttg accaggcagg tcaggctgac ttggttcttg
     3481 gtcatctcct cccgggatgg gggcagggtg tacacctgtg gttctcgggg ctggatcccc
     3541 gggaattccc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3601 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3661 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3721 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3781 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3841 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3901 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3961 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4021 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4081 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4141 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     4201 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4261 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4321 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4381 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag
     4441 ttttattgtt catgcctcta gaggctgcag ccgaccccgg atccggagct tttagagcag
     4501 aagtaacact tccgtacagg cctagaagta aaggcaacat ccactgagga gcagttcttt
     4561 gatttgcacc accaggagcc tcaaattttt caataaattc acctgactgc acattaggac
     4621 tttggctttg agcttcccac ctctcagtta cttgctgaat actgtctgct tcatcaatat
     4681 tatcataggt gtgcccaaat gatatttgca acccttccct gttggctact tgtctcacca
     4741 ttgtaggcct aatgggagac aaagtagagt agtaatcttg taaagagtta taccaattaa
     4801 caggagcatt aattactgtc caagtagttt cctctaaaaa ccttgccaaa ctgtccctta
     4861 aatatctttg ggttcttctt tcaagctcct gtgaggtgag cctaggaatg tcattttgta
     4921 ttacacgcca aaaagcttga gaaatggcat taaaaagtgt tggaccccaa tgtctggggt
     4981 caagatactg aacactgtga acaaaggttt gtactccagg aaataaaata tcatagtaat
     5041 catctggcct atacaaatct acagccattc ctggttgttg atataaacca acagtagaaa
     5101 ctttgtgatc ccagtcacta aaaaatctat accccacttg agcaacagcg ctcacaccag
     5161 tcacagtttg cagtaaagct gcaaatccag ctatagcagc aggagcccca gatatcacag
     5221 cataggcctg tggagtgagg cctatagcag caattgcctc agaggttgtt aggccttcaa
     5281 cagtagcaac agatgcaagt tgcacttcaa ttgcagcagc ggcctctcca gcagcaattt
     5341 cagctactga aaatccagta gcagcagcag cttcagacac agtagcaatt aggtccccca
     5401 acagtgttaa agcagcaccc atggacctga aataaaagac aaaaagacta aacttaccag
     5461 ttaactttct ggtttttcag ttaacctttc tggttttttg cgtttcccgt caacagtatc
     5521 ttccccttca caaaattgca gcaaaagctc taaaacaaac acaaaaaggc gttgagctgt
     5581 ttttttactt tcagtccatg acctacgaac cttaacggag gcctggcgtg acagccggcg
     5641 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg
     5701 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5761 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5821 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5881 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6001 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttgca
     6061 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     6121 gaaaggagtg cctgggggaa tattcctctg atgagaaagg catatttaaa aaaatgcaag
     6181 gagtttcatc ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa tactctgtac
     6241 aagaaaatgg aagatggagt aaaatatgct catcaacctg actttggagg cttctgggat
     6301 gcaactgagg tatttgcttc ttccttaaat cctggtgttg atgcaatgta ctgcaaacaa
     6361 tggcctgagt gtgcaaagaa aatgtctgct aactgcatat gcttgctgtg cttactgagg
     6421 atgaagcatg aaaatagaaa attatacagg aaagatccac ttgtgtgggt tgattgctac
     6481 tgcttcgatt gctttagaat gtggtttgga cttgatcttt gtgaaggaac cttacttctg
     6541 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     6601 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     6661 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     6721 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     6781 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     6841 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     6901 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     6961 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     7021 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     7081 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatccat tttctgttat
     7141 tgaggaaagt ttgccaggtg ggttaaagga gcatgatttt aatccagaag aagcagagga
     7201 aactaaacaa gtgtcctgga agcttgtaac agagtatgca atggaaacaa aatgtgatga
     7261 tgtgttgtta ttgcttggga tgtacttgga atttcagtac agttttgaaa tgtgtttaaa
     7321 atgtattaaa aaagaacagc ccagccacta taagtaccat gaaaagcatt atgcaaatgc
     7381 tgctatattt gctgacagca aaaaccaaaa aaccatatgc caacaggctg ttgatactgt
     7441 tttagctaaa aagcgggttg atagcctaca attaactaga gaacaaatgt taacaaacag
     7501 atttaatgat cttttggata ggatggatat aatgtttggt tctacaggct ctgctgacat
     7561 agaagaatgg atggctggag ttgcttggct acactgtttg ttgcccaaaa tggattcagt
     7621 ggtgtatgac tttttaaaat gcatggtgta caacattcct aaaaaaagat actggctgtt
     7681 taaaggacca attgatagtg gtaaaactac attagcagct gcctcgcgcg tttcggtgat
     7741 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     7801 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     7861 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     7921 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     7981 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8161 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8821 aaaactcacg ttaagggatt ttggtcatga tgatatattt ttatcttgtg caatgtaaca
     8881 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgac
     8941 tccccgcgcg cgatgggtcg aatttgcttt cgaaaaaaaa gcccgctcat taggcgggct
     9001 aaaaaaaagc ccgctcatta ggcgggctcg aatttctgcc attcatccgc ttattatcac
     9061 ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
     9121 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     9181 agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     9241 gcgtataata tttgcccata gtgaaaacgg gggcgaagaa gttgtccata ttcgccacgt
     9301 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     9361 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     9421 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     9481 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     9541 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     9601 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     9661 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     9721 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     9781 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     9841 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
     9901 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     9961 cttccgtcac aggtatttat tcgaagacga aagggcatcg cgcgcgggga a
//
