LOCUS       V002936                10022 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002936
VERSION     V002936
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10022)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3099..3229)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(3230..3553)
                     /codon_start=1
                     /note="unnamed protein product; hIG1; fragment of heavy
                     chain of human IG1"
                     /protein_id="SJL88041.1"
                     /translation="GQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNG
                     QPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLS
                     PGK"
     CDS             complement(3560..4417)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(4531..5468)
                     /label="intron"
                     /note="intron"
     CDS             complement(5475..5660)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5732..6061
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6075..6596
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6796..6816
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7871..8011
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8197..8785)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9130..9786)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9787..9889)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagtcgacca ggggcccagg gcagcgctgg
     3121 gtgctttatt tccatgctgg gcgcccggga agtatgtaca gggggtacgt gccaagcatc
     3181 ctcgtgcgac cgcgagagcc cggggagcgg ggcttgccgg ccgtcgcact catttacccg
     3241 gagacaggga gaggctcttc tgcgtgtagt ggttgtgcag agcctcatgc atcacggagc
     3301 atgagaagac gttcccctgc tgccacctgc tcttgtccac ggtgagcttg ctgtagagga
     3361 agaaggagcc gtcggagtcc agcacgggag gcgtggtctt gtagttgttc tccggctgcc
     3421 cattgctctc ccactccacg gcgatgtcgc tgggatagaa gcctttgacc aggcaggtca
     3481 ggctgacctg gttcttggtc agctcatccc gggatggggg cagggtgtac acctgtggtt
     3541 ctcggggctg cccgaattcc caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3841 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     3901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4141 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     4201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4441 gaagcagaca gttttattgt tcatgcctct agaggctgca gccgaccccg gatccggagc
     4501 ttttagagca gaagtaacac ttccgtacag gcctagaagt aaaggcaaca tccactgagg
     4561 agcagttctt tgatttgcac caccaggagc ctcaaatttt tcaataaatt cacctgactg
     4621 cacattagga ctttggcttt gagcttccca cctctcagtt acttgctgaa tactgtctgc
     4681 ttcatcaata ttatcatagg tgtgcccaaa tgatatttgc aacccttccc tgttggctac
     4741 ttgtctcacc attgtaggcc taatgggaga caaagtagag tagtaatctt gtaaagagtt
     4801 ataccaatta acaggagcat taattactgt ccaagtagtt tcctctaaaa accttgccaa
     4861 actgtccctt aaatatcttt gggttcttct ttcaagctcc tgtgaggtga gcctaggaat
     4921 gtcattttgt attacacgcc aaaaagcttg agaaatggca ttaaaaagtg ttggacccca
     4981 atgtctgggg tcaagatact gaacactgtg aacaaaggtt tgtactccag gaaataaaat
     5041 atcatagtaa tcatctggcc tatacaaatc tacagccatt cctggttgtt gatataaacc
     5101 aacagtagaa actttgtgat cccagtcact aaaaaatcta taccccactt gagcaacagc
     5161 gctcacacca gtcacagttt gcagtaaagc tgcaaatcca gctatagcag caggagcccc
     5221 agatatcaca gcataggcct gtggagtgag gcctatagca gcaattgcct cagaggttgt
     5281 taggccttca acagtagcaa cagatgcaag ttgcacttca attgcagcag cggcctctcc
     5341 agcagcaatt tcagctactg aaaatccagt agcagcagca gcttcagaca cagtagcaat
     5401 taggtccccc aacagtgtta aagcagcacc catggacctg aaataaaaga caaaaagact
     5461 aaacttacca gttaactttc tggtttttca gttaaccttt ctggtttttt gcgtttcccg
     5521 tcaacagtat cttccccttc acaaaattgc agcaaaagct ctaaaacaaa cacaaaaagg
     5581 cgttgagctg tttttttact ttcagtccat gacctacgaa ccttaacgga ggcctggcgt
     5641 gacagccggc gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac
     5701 cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5761 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5821 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5881 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5941 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6001 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6061 aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
     6121 ttctaggtct tgaaaggagt gcctggggga atattcctct gatgagaaag gcatatttaa
     6181 aaaaatgcaa ggagtttcat cctgataaag gaggagatga agaaaaaatg aagaaaatga
     6241 atactctgta caagaaaatg gaagatggag taaaatatgc tcatcaacct gactttggag
     6301 gcttctggga tgcaactgag gtatttgctt cttccttaaa tcctggtgtt gatgcaatgt
     6361 actgcaaaca atggcctgag tgtgcaaaga aaatgtctgc taactgcata tgcttgctgt
     6421 gcttactgag gatgaagcat gaaaatagaa aattatacag gaaagatcca cttgtgtggg
     6481 ttgattgcta ctgcttcgat tgctttagaa tgtggtttgg acttgatctt tgtgaaggaa
     6541 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     6601 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     6661 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     6721 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     6781 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     6841 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     6901 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     6961 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     7021 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     7081 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcca
     7141 ttttctgtta ttgaggaaag tttgccaggt gggttaaagg agcatgattt taatccagaa
     7201 gaagcagagg aaactaaaca agtgtcctgg aagcttgtaa cagagtatgc aatggaaaca
     7261 aaatgtgatg atgtgttgtt attgcttggg atgtacttgg aatttcagta cagttttgaa
     7321 atgtgtttaa aatgtattaa aaaagaacag cccagccact ataagtacca tgaaaagcat
     7381 tatgcaaatg ctgctatatt tgctgacagc aaaaaccaaa aaaccatatg ccaacaggct
     7441 gttgatactg ttttagctaa aaagcgggtt gatagcctac aattaactag agaacaaatg
     7501 ttaacaaaca gatttaatga tcttttggat aggatggata taatgtttgg ttctacaggc
     7561 tctgctgaca tagaagaatg gatggctgga gttgcttggc tacactgttt gttgcccaaa
     7621 atggattcag tggtgtatga ctttttaaaa tgcatggtgt acaacattcc taaaaaaaga
     7681 tactggctgt ttaaaggacc aattgatagt ggtaaaacta cattagcagc tgcctcgcgc
     7741 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7801 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7861 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     7921 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     7981 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     8041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8161 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8821 tcagtggaac gaaaactcac gttaagggat tttggtcatg atgatatatt tttatcttgt
     8881 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt
     8941 gctgagttga ctccccgcgc gcgatgggtc gaatttgctt tcgaaaaaaa agcccgctca
     9001 ttaggcgggc taaaaaaaag cccgctcatt aggcgggctc gaatttctgc cattcatccg
     9061 cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta
     9121 aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     9181 gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac
     9241 cttgtcgcct tgcgtataat atttgcccat agtgaaaacg ggggcgaaga agttgtccat
     9301 attcgccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     9361 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     9421 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     9481 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     9541 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     9601 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     9661 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     9721 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     9781 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     9841 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt
     9901 tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc
     9961 tgcgaagtga tcttccgtca caggtattta ttcgaagacg aaagggcatc gcgcgcgggg
    10021 aa
//
