LOCUS       V002928                10377 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002928
VERSION     V002928
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10377)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10377)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3104..3194)
                     /label="3' UTR of mIgG2b"
                     /note="3' UTR of mIgG2b"
     CDS             complement(3195..3914)
                     /codon_start=1
                     /note="unnamed protein product; mIgG2b; fragment of heavy
                     chain of mouse anti-hPLAP monoclonal antibody (MG2)"
                     /protein_id="SJL88232.1"
                     /translation="ELSGPTSTINPCPPCKECHKCPAPNLEGGPSVFIFPPNIKDVLMI
                     SLTPKVTCVVVDVSEDDPDVQISWFVNNVEVLTAQTQTHREDYNSTIRVVSALPIQHQD
                     WMSGKEFKCKVNNKDLPAPIERTISKIKGIVRAPQVYILSPPPEQLSRKDVSLTCLAVG
                     FSPEDISVEWTSNGHTEENYKDKGPVLDSDGSYFIYSKLNMKTSKWEKTDSFSCNVRHE
                     GLKNYYLKKTISRSPGK"
     CDS             complement(3915..4772)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(4886..5823)
                     /label="intron"
                     /note="intron"
     CDS             complement(5830..6015)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        6087..6416
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6430..6951
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             7151..7171
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    8226..8366
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8552..9140)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9485..10141)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(10142..10244)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagtcgactc tagcaggctt tgctggatgc
     3121 tttatttata caagggatgc atggatatgg gtgccagtgt ctcttaggac ctgagagctt
     3181 tgtgggtgct gagctcattt acccggagac cgggagatgg tcttcttcag gtagtaattt
     3241 ttcagaccct cgtgtctcac gttgcatgag aaggaatctg ttttctccca cttgcttgtt
     3301 ttcatattga gcttgctata tatgaagtaa gaaccgtcag agtctaggac tgggcctttg
     3361 tccttgtagt tctcctctgt atgcccattg ctggtccact ccacactgat gtcttcaggg
     3421 ctgaagccca cggccaggca agtcagactg acatctttcc tggacaactg ctctggtggt
     3481 ggcgacaaga tgtatacttg tggagctctg actatccctt taatttttga gatggttctc
     3541 tcaatgggcg ctgggaggtc tttgttgttg accttgcatt tgaactcctt gccactcatc
     3601 cagtcctggt gctggatggg gagggcactg accacccgga tagtactgtt gtaatcctct
     3661 ctatgggttt gtgtctgagc tgtgagtact tccacgttgt tcacaaacca gctgatctgg
     3721 acgtctgggt catcctcgct cacatccacc accacacacg tgaccttggg tgtcagggag
     3781 atcatgagta catccttgat atttggaggg aagatgaaga cggatggtcc accctcgagg
     3841 ttaggagctg ggcatttgtg acactccttg catggaggac aggggttgat tgttgaagtg
     3901 ggccctgaga gctcccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3961 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4021 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4081 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4141 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4201 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     4261 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4321 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4381 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4441 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4501 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     4561 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4621 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4681 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4741 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4801 agacagtttt attgttcatg cctctagagg ctgcagccga ccccggatcc ggagctttta
     4861 gagcagaagt aacacttccg tacaggccta gaagtaaagg caacatccac tgaggagcag
     4921 ttctttgatt tgcaccacca ggagcctcaa atttttcaat aaattcacct gactgcacat
     4981 taggactttg gctttgagct tcccacctct cagttacttg ctgaatactg tctgcttcat
     5041 caatattatc ataggtgtgc ccaaatgata tttgcaaccc ttccctgttg gctacttgtc
     5101 tcaccattgt aggcctaatg ggagacaaag tagagtagta atcttgtaaa gagttatacc
     5161 aattaacagg agcattaatt actgtccaag tagtttcctc taaaaacctt gccaaactgt
     5221 cccttaaata tctttgggtt cttctttcaa gctcctgtga ggtgagccta ggaatgtcat
     5281 tttgtattac acgccaaaaa gcttgagaaa tggcattaaa aagtgttgga ccccaatgtc
     5341 tggggtcaag atactgaaca ctgtgaacaa aggtttgtac tccaggaaat aaaatatcat
     5401 agtaatcatc tggcctatac aaatctacag ccattcctgg ttgttgatat aaaccaacag
     5461 tagaaacttt gtgatcccag tcactaaaaa atctataccc cacttgagca acagcgctca
     5521 caccagtcac agtttgcagt aaagctgcaa atccagctat agcagcagga gccccagata
     5581 tcacagcata ggcctgtgga gtgaggccta tagcagcaat tgcctcagag gttgttaggc
     5641 cttcaacagt agcaacagat gcaagttgca cttcaattgc agcagcggcc tctccagcag
     5701 caatttcagc tactgaaaat ccagtagcag cagcagcttc agacacagta gcaattaggt
     5761 cccccaacag tgttaaagca gcacccatgg acctgaaata aaagacaaaa agactaaact
     5821 taccagttaa ctttctggtt tttcagttaa cctttctggt tttttgcgtt tcccgtcaac
     5881 agtatcttcc ccttcacaaa attgcagcaa aagctctaaa acaaacacaa aaaggcgttg
     5941 agctgttttt ttactttcag tccatgacct acgaacctta acggaggcct ggcgtgacag
     6001 ccggcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct
     6061 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6121 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6181 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6241 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6301 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6361 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6421 tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     6481 ggtcttgaaa ggagtgcctg ggggaatatt cctctgatga gaaaggcata tttaaaaaaa
     6541 tgcaaggagt ttcatcctga taaaggagga gatgaagaaa aaatgaagaa aatgaatact
     6601 ctgtacaaga aaatggaaga tggagtaaaa tatgctcatc aacctgactt tggaggcttc
     6661 tgggatgcaa ctgaggtatt tgcttcttcc ttaaatcctg gtgttgatgc aatgtactgc
     6721 aaacaatggc ctgagtgtgc aaagaaaatg tctgctaact gcatatgctt gctgtgctta
     6781 ctgaggatga agcatgaaaa tagaaaatta tacaggaaag atccacttgt gtgggttgat
     6841 tgctactgct tcgattgctt tagaatgtgg tttggacttg atctttgtga aggaacctta
     6901 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     6961 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     7021 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     7081 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     7141 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     7201 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     7261 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     7321 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     7381 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     7441 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atccattttc
     7501 tgttattgag gaaagtttgc caggtgggtt aaaggagcat gattttaatc cagaagaagc
     7561 agaggaaact aaacaagtgt cctggaagct tgtaacagag tatgcaatgg aaacaaaatg
     7621 tgatgatgtg ttgttattgc ttgggatgta cttggaattt cagtacagtt ttgaaatgtg
     7681 tttaaaatgt attaaaaaag aacagcccag ccactataag taccatgaaa agcattatgc
     7741 aaatgctgct atatttgctg acagcaaaaa ccaaaaaacc atatgccaac aggctgttga
     7801 tactgtttta gctaaaaagc gggttgatag cctacaatta actagagaac aaatgttaac
     7861 aaacagattt aatgatcttt tggataggat ggatataatg tttggttcta caggctctgc
     7921 tgacatagaa gaatggatgg ctggagttgc ttggctacac tgtttgttgc ccaaaatgga
     7981 ttcagtggtg tatgactttt taaaatgcat ggtgtacaac attcctaaaa aaagatactg
     8041 gctgtttaaa ggaccaattg atagtggtaa aactacatta gcagctgcct cgcgcgtttc
     8101 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     8161 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     8221 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     8281 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     8341 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     8401 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8461 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8521 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8581 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8641 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8701 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8761 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8821 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8881 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8941 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9001 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9061 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9181 ggaacgaaaa ctcacgttaa gggattttgg tcatgatgat atatttttat cttgtgcaat
     9241 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
     9301 gttgactccc cgcgcgcgat gggtcgaatt tgctttcgaa aaaaaagccc gctcattagg
     9361 cgggctaaaa aaaagcccgc tcattaggcg ggctcgaatt tctgccattc atccgcttat
     9421 tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa
     9481 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     9541 catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     9601 cgccttgcgt ataatatttg cccatagtga aaacgggggc gaagaagttg tccatattcg
     9661 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     9721 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     9781 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     9841 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     9901 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     9961 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
    10021 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
    10081 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
    10141 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
    10201 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
    10261 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
    10321 agtgatcttc cgtcacaggt atttattcga agacgaaagg gcatcgcgcg cggggaa
//
