LOCUS       V002930                 9866 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002930
VERSION     V002930
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9866)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3188..3373)
                     /codon_start=1
                     /note="unnamed protein product; H1-4"
                     /protein_id="SJL88747.1"
                     /translation="ELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPR
                     CPEPKSCDTPPPCPRCP"
     misc_feature    complement(3374..3402)
                     /label="linker"
                     /note="linker"
     CDS             complement(3404..4261)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(4375..5312)
                     /label="intron"
                     /note="intron"
     CDS             complement(5319..5504)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5576..5905
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5919..6440
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6640..6660
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7715..7855
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8041..8629)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8974..9630)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9631..9733)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gaggcatgag attatcaaaa aggatcttca
     3121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3181 cgggccctgg gcaccttggg cacgggggag gtgtgtcaca agatttgggc tctgggcacc
     3241 gtgggcatgg gggaggtgtg tcacaagatt tgggctctgg gcaccgtggg cacgggggag
     3301 gtgtgtcaca agatttgggc tctgggcacc gtgggcatgt gtgagttgtg tcaccaagtg
     3361 gggttttgag ctctggaggc ttccccagtc ctgggcccga ctcccaatgc ttaatcagtg
     3421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     3721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     4021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4261 tactcttcct ttttcaatat tattgaagca gacagtttta ttgttcatgc ctctagaggc
     4321 tgcagccgac cccggatccg gagcttttag agcagaagta acacttccgt acaggcctag
     4381 aagtaaaggc aacatccact gaggagcagt tctttgattt gcaccaccag gagcctcaaa
     4441 tttttcaata aattcacctg actgcacatt aggactttgg ctttgagctt cccacctctc
     4501 agttacttgc tgaatactgt ctgcttcatc aatattatca taggtgtgcc caaatgatat
     4561 ttgcaaccct tccctgttgg ctacttgtct caccattgta ggcctaatgg gagacaaagt
     4621 agagtagtaa tcttgtaaag agttatacca attaacagga gcattaatta ctgtccaagt
     4681 agtttcctct aaaaaccttg ccaaactgtc ccttaaatat ctttgggttc ttctttcaag
     4741 ctcctgtgag gtgagcctag gaatgtcatt ttgtattaca cgccaaaaag cttgagaaat
     4801 ggcattaaaa agtgttggac cccaatgtct ggggtcaaga tactgaacac tgtgaacaaa
     4861 ggtttgtact ccaggaaata aaatatcata gtaatcatct ggcctataca aatctacagc
     4921 cattcctggt tgttgatata aaccaacagt agaaactttg tgatcccagt cactaaaaaa
     4981 tctatacccc acttgagcaa cagcgctcac accagtcaca gtttgcagta aagctgcaaa
     5041 tccagctata gcagcaggag ccccagatat cacagcatag gcctgtggag tgaggcctat
     5101 agcagcaatt gcctcagagg ttgttaggcc ttcaacagta gcaacagatg caagttgcac
     5161 ttcaattgca gcagcggcct ctccagcagc aatttcagct actgaaaatc cagtagcagc
     5221 agcagcttca gacacagtag caattaggtc ccccaacagt gttaaagcag cacccatgga
     5281 cctgaaataa aagacaaaaa gactaaactt accagttaac tttctggttt ttcagttaac
     5341 ctttctggtt ttttgcgttt cccgtcaaca gtatcttccc cttcacaaaa ttgcagcaaa
     5401 agctctaaaa caaacacaaa aaggcgttga gctgtttttt tactttcagt ccatgaccta
     5461 cgaaccttaa cggaggcctg gcgtgacagc cggcgcagca ccatggcctg aaataacctc
     5521 tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     5581 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5641 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5701 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5761 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5821 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5881 ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt ttaaacagag
     5941 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg gggaatattc
     6001 ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat aaaggaggag
     6061 atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat ggagtaaaat
     6121 atgctcatca acctgacttt ggaggcttct gggatgcaac tgaggtattt gcttcttcct
     6181 taaatcctgg tgttgatgca atgtactgca aacaatggcc tgagtgtgca aagaaaatgt
     6241 ctgctaactg catatgcttg ctgtgcttac tgaggatgaa gcatgaaaat agaaaattat
     6301 acaggaaaga tccacttgtg tgggttgatt gctactgctt cgattgcttt agaatgtggt
     6361 ttggacttga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     6421 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     6481 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     6541 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     6601 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     6661 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     6721 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     6781 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     6841 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     6901 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     6961 tatagtgcct tgactagaga tccattttct gttattgagg aaagtttgcc aggtgggtta
     7021 aaggagcatg attttaatcc agaagaagca gaggaaacta aacaagtgtc ctggaagctt
     7081 gtaacagagt atgcaatgga aacaaaatgt gatgatgtgt tgttattgct tgggatgtac
     7141 ttggaatttc agtacagttt tgaaatgtgt ttaaaatgta ttaaaaaaga acagcccagc
     7201 cactataagt accatgaaaa gcattatgca aatgctgcta tatttgctga cagcaaaaac
     7261 caaaaaacca tatgccaaca ggctgttgat actgttttag ctaaaaagcg ggttgatagc
     7321 ctacaattaa ctagagaaca aatgttaaca aacagattta atgatctttt ggataggatg
     7381 gatataatgt ttggttctac aggctctgct gacatagaag aatggatggc tggagttgct
     7441 tggctacact gtttgttgcc caaaatggat tcagtggtgt atgacttttt aaaatgcatg
     7501 gtgtacaaca ttcctaaaaa aagatactgg ctgtttaaag gaccaattga tagtggtaaa
     7561 actacattag cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     7621 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7681 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     7741 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     7801 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     7861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8461 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8701 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     8761 ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg ggtcgaattt
     8821 gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct cattaggcgg
     8881 gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt
     8941 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta
     9001 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac
     9061 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatagtgaa
     9121 aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg tgaaactcac
     9181 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     9241 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     9301 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     9361 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     9421 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     9481 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     9541 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     9601 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     9661 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     9721 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
     9781 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcgaa
     9841 gacgaaaggg catcgcgcgc ggggaa
//
