LOCUS       V002931                 9878 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002931
VERSION     V002931
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9878)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9878)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3188..3373)
                     /codon_start=1
                     /note="unnamed protein product; H1-4"
                     /protein_id="SJL88742.1"
                     /translation="ELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPR
                     CPEPKSCDTPPPCPRCP"
     misc_feature    complement(3374..3414)
                     /label="linker"
                     /note="linker"
     CDS             complement(3416..4273)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(4387..5324)
                     /label="intron"
                     /note="intron"
     CDS             complement(5331..5516)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5588..5917
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5931..6452
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6652..6672
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7727..7867
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8053..8641)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8986..9642)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9643..9745)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gaggcatgag attatcaaaa aggatcttca
     3121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3181 cgggccctgg gcaccttggg cacgggggag gtgtgtcaca agatttgggc tctgggcacc
     3241 gtgggcatgg gggaggtgtg tcacaagatt tgggctctgg gcaccgtggg cacgggggag
     3301 gtgtgtcaca agatttgggc tctgggcacc gtgggcatgt gtgagttgtg tcaccaagtg
     3361 gggttttgag ctcaactctc ttgtccacct tggtgttgct gggcttgtga ttcacccaat
     3421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3721 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4021 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4261 gttgaatact catactcttc ctttttcaat attattgaag cagacagttt tattgttcat
     4321 gcctctagag gctgcagccg accccggatc cggagctttt agagcagaag taacacttcc
     4381 gtacaggcct agaagtaaag gcaacatcca ctgaggagca gttctttgat ttgcaccacc
     4441 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc
     4501 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg
     4561 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat
     4621 gggagacaaa gtagagtagt aatcttgtaa agagttatac caattaacag gagcattaat
     4681 tactgtccaa gtagtttcct ctaaaaacct tgccaaactg tcccttaaat atctttgggt
     4741 tcttctttca agctcctgtg aggtgagcct aggaatgtca ttttgtatta cacgccaaaa
     4801 agcttgagaa atggcattaa aaagtgttgg accccaatgt ctggggtcaa gatactgaac
     4861 actgtgaaca aaggtttgta ctccaggaaa taaaatatca tagtaatcat ctggcctata
     4921 caaatctaca gccattcctg gttgttgata taaaccaaca gtagaaactt tgtgatccca
     4981 gtcactaaaa aatctatacc ccacttgagc aacagcgctc acaccagtca cagtttgcag
     5041 taaagctgca aatccagcta tagcagcagg agccccagat atcacagcat aggcctgtgg
     5101 agtgaggcct atagcagcaa ttgcctcaga ggttgttagg ccttcaacag tagcaacaga
     5161 tgcaagttgc acttcaattg cagcagcggc ctctccagca gcaatttcag ctactgaaaa
     5221 tccagtagca gcagcagctt cagacacagt agcaattagg tcccccaaca gtgttaaagc
     5281 agcacccatg gacctgaaat aaaagacaaa aagactaaac ttaccagtta actttctggt
     5341 ttttcagtta acctttctgg ttttttgcgt ttcccgtcaa cagtatcttc cccttcacaa
     5401 aattgcagca aaagctctaa aacaaacaca aaaaggcgtt gagctgtttt tttactttca
     5461 gtccatgacc tacgaacctt aacggaggcc tggcgtgaca gccggcgcag caccatggcc
     5521 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     5581 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5641 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5761 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5821 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5881 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttgcaaag atggataaag
     5941 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct
     6001 gggggaatat tcctctgatg agaaaggcat atttaaaaaa atgcaaggag tttcatcctg
     6061 ataaaggagg agatgaagaa aaaatgaaga aaatgaatac tctgtacaag aaaatggaag
     6121 atggagtaaa atatgctcat caacctgact ttggaggctt ctgggatgca actgaggtat
     6181 ttgcttcttc cttaaatcct ggtgttgatg caatgtactg caaacaatgg cctgagtgtg
     6241 caaagaaaat gtctgctaac tgcatatgct tgctgtgctt actgaggatg aagcatgaaa
     6301 atagaaaatt atacaggaaa gatccacttg tgtgggttga ttgctactgc ttcgattgct
     6361 ttagaatgtg gtttggactt gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     6421 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     6481 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     6541 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     6601 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     6661 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     6721 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     6781 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     6841 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     6901 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     6961 gaatatttga tgtatagtgc cttgactaga gatccatttt ctgttattga ggaaagtttg
     7021 ccaggtgggt taaaggagca tgattttaat ccagaagaag cagaggaaac taaacaagtg
     7081 tcctggaagc ttgtaacaga gtatgcaatg gaaacaaaat gtgatgatgt gttgttattg
     7141 cttgggatgt acttggaatt tcagtacagt tttgaaatgt gtttaaaatg tattaaaaaa
     7201 gaacagccca gccactataa gtaccatgaa aagcattatg caaatgctgc tatatttgct
     7261 gacagcaaaa accaaaaaac catatgccaa caggctgttg atactgtttt agctaaaaag
     7321 cgggttgata gcctacaatt aactagagaa caaatgttaa caaacagatt taatgatctt
     7381 ttggatagga tggatataat gtttggttct acaggctctg ctgacataga agaatggatg
     7441 gctggagttg cttggctaca ctgtttgttg cccaaaatgg attcagtggt gtatgacttt
     7501 ttaaaatgca tggtgtacaa cattcctaaa aaaagatact ggctgtttaa aggaccaatt
     7561 gatagtggta aaactacatt agcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     7621 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7681 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     7741 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     7801 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7861 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7921 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7981 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8041 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8221 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8341 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8401 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8461 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8521 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8581 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8641 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8701 agggattttg gtcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     8761 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgactcc ccgcgcgcga
     8821 tgggtcgaat ttgctttcga aaaaaaagcc cgctcattag gcgggctaaa aaaaagcccg
     8881 ctcattaggc gggctcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta
     8941 gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
     9001 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
     9061 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     9121 gcccatagtg aaaacggggg cgaagaagtt gtccatattc gccacgttta aatcaaaact
     9181 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     9241 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     9301 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     9361 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     9421 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     9481 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     9541 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     9601 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     9661 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     9721 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt
     9781 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg
     9841 tatttattcg aagacgaaag ggcatcgcgc gcggggaa
//
