LOCUS       V002932                 9836 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002932
VERSION     V002932
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9836)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9836)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3188..3373)
                     /codon_start=1
                     /note="unnamed protein product; H1-4"
                     /protein_id="SJL88737.1"
                     /translation="ELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPR
                     CPEPKSCDTPPPCPRCP"
     CDS             complement(3374..4231)
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(4345..5282)
                     /label="intron"
                     /note="intron"
     CDS             complement(5289..5474)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5546..5875
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5889..6410
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6610..6630
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7685..7825
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8011..8599)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8944..9600)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9601..9703)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gaggcatgag attatcaaaa aggatcttca
     3121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3181 cgggccctgg gcaccttggg cacgggggag gtgtgtcaca agatttgggc tctgggcacc
     3241 gtgggcatgg gggaggtgtg tcacaagatt tgggctctgg gcaccgtggg cacgggggag
     3301 gtgtgtcaca agatttgggc tctgggcacc gtgggcatgt gtgagttgtg tcaccaagtg
     3361 gggttttgag ctcccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3481 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3661 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     3721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3841 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3961 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     4021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4201 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4261 gacagtttta ttgttcatgc ctctagaggc tgcagccgac cccggatccg gagcttttag
     4321 agcagaagta acacttccgt acaggcctag aagtaaaggc aacatccact gaggagcagt
     4381 tctttgattt gcaccaccag gagcctcaaa tttttcaata aattcacctg actgcacatt
     4441 aggactttgg ctttgagctt cccacctctc agttacttgc tgaatactgt ctgcttcatc
     4501 aatattatca taggtgtgcc caaatgatat ttgcaaccct tccctgttgg ctacttgtct
     4561 caccattgta ggcctaatgg gagacaaagt agagtagtaa tcttgtaaag agttatacca
     4621 attaacagga gcattaatta ctgtccaagt agtttcctct aaaaaccttg ccaaactgtc
     4681 ccttaaatat ctttgggttc ttctttcaag ctcctgtgag gtgagcctag gaatgtcatt
     4741 ttgtattaca cgccaaaaag cttgagaaat ggcattaaaa agtgttggac cccaatgtct
     4801 ggggtcaaga tactgaacac tgtgaacaaa ggtttgtact ccaggaaata aaatatcata
     4861 gtaatcatct ggcctataca aatctacagc cattcctggt tgttgatata aaccaacagt
     4921 agaaactttg tgatcccagt cactaaaaaa tctatacccc acttgagcaa cagcgctcac
     4981 accagtcaca gtttgcagta aagctgcaaa tccagctata gcagcaggag ccccagatat
     5041 cacagcatag gcctgtggag tgaggcctat agcagcaatt gcctcagagg ttgttaggcc
     5101 ttcaacagta gcaacagatg caagttgcac ttcaattgca gcagcggcct ctccagcagc
     5161 aatttcagct actgaaaatc cagtagcagc agcagcttca gacacagtag caattaggtc
     5221 ccccaacagt gttaaagcag cacccatgga cctgaaataa aagacaaaaa gactaaactt
     5281 accagttaac tttctggttt ttcagttaac ctttctggtt ttttgcgttt cccgtcaaca
     5341 gtatcttccc cttcacaaaa ttgcagcaaa agctctaaaa caaacacaaa aaggcgttga
     5401 gctgtttttt tactttcagt ccatgaccta cgaaccttaa cggaggcctg gcgtgacagc
     5461 cggcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     5521 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5581 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5641 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5701 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5881 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     5941 gtcttgaaag gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat
     6001 gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc
     6061 tgtacaagaa aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct
     6121 gggatgcaac tgaggtattt gcttcttcct taaatcctgg tgttgatgca atgtactgca
     6181 aacaatggcc tgagtgtgca aagaaaatgt ctgctaactg catatgcttg ctgtgcttac
     6241 tgaggatgaa gcatgaaaat agaaaattat acaggaaaga tccacttgtg tgggttgatt
     6301 gctactgctt cgattgcttt agaatgtggt ttggacttga tctttgtgaa ggaaccttac
     6361 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     6421 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     6481 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     6541 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     6601 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     6661 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     6721 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     6781 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     6841 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     6901 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tccattttct
     6961 gttattgagg aaagtttgcc aggtgggtta aaggagcatg attttaatcc agaagaagca
     7021 gaggaaacta aacaagtgtc ctggaagctt gtaacagagt atgcaatgga aacaaaatgt
     7081 gatgatgtgt tgttattgct tgggatgtac ttggaatttc agtacagttt tgaaatgtgt
     7141 ttaaaatgta ttaaaaaaga acagcccagc cactataagt accatgaaaa gcattatgca
     7201 aatgctgcta tatttgctga cagcaaaaac caaaaaacca tatgccaaca ggctgttgat
     7261 actgttttag ctaaaaagcg ggttgatagc ctacaattaa ctagagaaca aatgttaaca
     7321 aacagattta atgatctttt ggataggatg gatataatgt ttggttctac aggctctgct
     7381 gacatagaag aatggatggc tggagttgct tggctacact gtttgttgcc caaaatggat
     7441 tcagtggtgt atgacttttt aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg
     7501 ctgtttaaag gaccaattga tagtggtaaa actacattag cagctgcctc gcgcgtttcg
     7561 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7621 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7681 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     7741 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     7801 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7921 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7981 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8041 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8101 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8161 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8221 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8281 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8341 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8401 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8461 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8521 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8641 gaacgaaaac tcacgttaag ggattttggt catgatgata tatttttatc ttgtgcaatg
     8701 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
     8761 ttgactcccc gcgcgcgatg ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc
     8821 gggctaaaaa aaagcccgct cattaggcgg gctcgaattt ctgccattca tccgcttatt
     8881 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
     8941 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     9001 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     9061 gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattcgc
     9121 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     9181 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     9241 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     9301 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     9361 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     9421 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     9481 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     9541 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     9601 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     9661 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
     9721 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
     9781 gtgatcttcc gtcacaggta tttattcgaa gacgaaaggg catcgcgcgc ggggaa
//
