LOCUS       V002937                 7729 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002937
VERSION     V002937
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7729)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    733..867
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1153..1650)
                     /gene="IFNG"
                     /label="Interferon gamma"
                     /note="Interferon gamma from Homo sapiens. Accession#:
                     P01579"
     misc_feature    complement(1747..2684)
                     /label="intron"
                     /note="intron"
     CDS             complement(2691..2876)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        2920..3277
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3291..3691
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88701.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPP"
     polyA_signal    complement(4129..4177)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4222..5256)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(5300..5445)
                     /label="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(5902..6490)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6664..7521)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7522..7626)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgttag gaccctttta ctaaccctaa ttcgatagca
       61 tatgcttccc gttgggtaac atatgctatt gaattagggt tagtctggat agtatatact
      121 actacccggg aagcatatgc tacccgttta gggttaacaa gggggcctta taaacactat
      181 tgctaatgcc ctcttgaggg tccgcttatc ggtagctaca caggcccctc tgattgacgt
      241 tggtgtagcc tcccgtagtc ttcctgggcc cctgggaggt acatgtcccc cagcattggt
      301 gtaagagctt cagccaagag ttacacataa aggcaatgtt gtgttgcagt ccacagactg
      361 caaagtctgc tccaggatga aagccactca gtgttggcaa atgtgcacat ccatttataa
      421 ggatgtcaac tacagtcaga gaaccccttt gtgtttggtc cccccccgtg tcacatgtgg
      481 aacagggccc agttggcaag ttgtaccaac caactgaagg gattacatgc actgccccgt
      541 gaccaataca aaacaaaagc gctcctcgta ccagcgaaga aggggcagag atgccgtagt
      601 caggtttagt tcgtccggcg gcgggggatc ataatcagcc ataccacatt tgtagaggtt
      661 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      721 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      781 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      841 atcaatgtat cttatcatgt ctggatcttg cttaggttgg ctgcctagtt ggcccctgag
      901 ataaagcctt gtaatcacat agccttgcct aattagtcag aaaacaaagg attaagtgag
      961 acagtcacag gatataggaa ttataaataa tacatatatt aatagatatt cattttcatt
     1021 acacaaaagt tgctattata aatacttatt tgattgatga gtctaaaaat atattcccca
     1081 tataaataat gttaaatatt aataaataga tttagattta aaattcaaat attgcaggca
     1141 ggacaaccat tactgggatg ctcttcgacc tcgaaacagc atctgactcc tttttcgctt
     1201 ccctgtttta gctgctggcg acagttcagc catcacttgg atgagttcat gtattgcttt
     1261 gcgttggaca ttcaagtcag ttaccgaata attagtcagc ttttcgaagt catctcgttt
     1321 ctttttgttg ctattgaaaa acttgacatt catgtcttcc ttgatggtct ccacactctt
     1381 ttggatgctc tggtcatctt taaagttttt aaaaagtttg aagtaaaagg agacaatttg
     1441 gctctgcatt atttttctgt cactctcctc tttccaattc ttcaaaatgc ctaagaaaag
     1501 agttccatta tccgctacat ctgaatgacc tgcattaaaa tatttcttaa ggttttctgc
     1561 ttcttttaca tatgggtcct ggcagtaaca gccaagagaa cccaaaacga tgcagagctg
     1621 aaaagccaag atataacttg tatatttcat cgtttccgag agaattaagc caaagaagtt
     1681 gaaatcagta gttcttgtat caagctgatc cggagctttt agagcagaag taacacttcc
     1741 gtacaggcct agaagtaaag gcaacatcca ctgaggagca gttctttgat ttgcaccacc
     1801 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc
     1861 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg
     1921 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat
     1981 gggagacaaa gtagagtagt aatcttgtaa agagttatac caattaacag gagcattaat
     2041 tactgtccaa gtagtttcct ctaaaaacct tgccaaactg tcccttaaat atctttgggt
     2101 tcttctttca agctcctgtg aggtgagcct aggaatgtca ttttgtatta cacgccaaaa
     2161 agcttgagaa atggcattaa aaagtgttgg accccaatgt ctggggtcaa gatactgaac
     2221 actgtgaaca aaggtttgta ctccaggaaa taaaatatca tagtaatcat ctggcctata
     2281 caaatctaca gccattcctg gttgttgata taaaccaaca gtagaaactt tgtgatccca
     2341 gtcactaaaa aatctatacc ccacttgagc aacagcgctc acaccagtca cagtttgcag
     2401 taaagctgca aatccagcta tagcagcagg agccccagat atcacagcat aggcctgtgg
     2461 agtgaggcct atagcagcaa ttgcctcaga ggttgttagg ccttcaacag tagcaacaga
     2521 tgcaagttgc acttcaattg cagcagcggc ctctccagca gcaatttcag ctactgaaaa
     2581 tccagtagca gcagcagctt cagacacagt agcaattagg tcccccaaca gtgttaaagc
     2641 agcacccatg gacctgaaat aaaagacaaa aagactaaac ttaccagtta actttctggt
     2701 ttttcagtta acctttctgg ttttttgcgt ttcccgtcaa cagtatcttc cccttcacaa
     2761 aattgcagca aaagctctaa aacaaacaca aaaaggcgtt gagctgtttt tttactttca
     2821 gtccatgacc tacgaacctt aacggaggcc tggcgtgaca gccggcgcag caccatggcc
     2881 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     2941 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3001 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3061 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3121 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3181 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3241 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttgcaaag atggataaag
     3301 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct
     3361 gggggaatat tcctctgatg agaaaggcat atttaaaaaa atgcaaggag tttcatcctg
     3421 ataaaggagg agatgaagaa aaaatgaaga aaatgaatac tctgtacaag aaaatggaag
     3481 atggagtaaa atatgctcat caacctgact ttggaggctt ctgggatgca actgaggtat
     3541 ttgcttcttc cttaaatcct ggtgttgatg caatgtactg caaacaatgg cctgagtgtg
     3601 caaagaaaat gtctgctaac tgcatatgct tgctgtgctt actgaggatg aagcatgaaa
     3661 atagaaaatt atacaggaaa gatccaccag atcctctaga gtcgacctgc agcccaagct
     3721 tggcactggc gccagaaatc cgcgcggtgg tttttggggg tcgggggtgt ttggcagcca
     3781 cagacgcccg gtgttcgtgt cgcgccagta catgcggtcc atgcccaggc catccaaaaa
     3841 ccatgggtct gtctgctcag tccagtcgtg gacctgaccc cacgcaacgc ccaaaagaat
     3901 aacccccacg aaccataaac cattccccat gggggacccc gtccctaacc cacggggccc
     3961 gtggctatgg cgggcttgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac
     4021 ttgggggttg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg
     4081 gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc
     4141 caacacccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
     4201 attgtctcct tccgtgtttc agttagcctc ccccatctcc cgatccccac gagtgctggg
     4261 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     4321 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     4381 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     4441 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     4501 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     4561 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     4621 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     4681 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     4741 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     4801 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     4861 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     4921 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     4981 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     5041 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     5101 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     5161 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     5221 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc ggggatcttg
     5281 cggcacgctg ttgacgctgt taagcgggtc gctgcagggt cgctcggtgt tcgaggccac
     5341 acgcgtcacc ttaatatgcg aagtggacct gggaccgcgc cgccccgact gcatctgcgt
     5401 gttcgaattc gccaatgaca agacgctggg cggggtttgt gtcatcatag aactaaagac
     5461 atgcaaatat atttcttccg gggacaccgc cagcaaacgc gagcaacggg ccacggggat
     5521 gaagcagccc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     5581 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     5641 gccatctcca gcagccgcac gcggcgcatc tcggggccga cgcgctgggc tacgtcttgc
     5701 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     5761 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     5821 gacagcttca aggatcgctc gcggctctta ccagcgccag caaaaggcca ggaaccgtaa
     5881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6301 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6421 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6481 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6661 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6721 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6781 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6841 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6901 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6961 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7021 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7081 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7141 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7201 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7261 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     7321 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7381 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7441 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7501 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7561 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7621 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     7681 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//
