LOCUS       V002938                 7729 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002938
VERSION     V002938
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7729)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(629..1029)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88696.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPP"
     promoter        complement(1043..1400)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             1444..1629
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     misc_feature    1636..2573
                     /label="intron"
                     /note="intron"
     CDS             2670..3167
                     /gene="IFNG"
                     /label="Interferon gamma"
                     /note="Interferon gamma from Homo sapiens. Accession#:
                     P01579"
     polyA_signal    complement(3453..3587)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(4129..4177)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4222..5256)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(5300..5445)
                     /label="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(5902..6490)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6664..7521)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7522..7626)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgttag gaccctttta ctaaccctaa ttcgatagca
       61 tatgcttccc gttgggtaac atatgctatt gaattagggt tagtctggat agtatatact
      121 actacccggg aagcatatgc tacccgttta gggttaacaa gggggcctta taaacactat
      181 tgctaatgcc ctcttgaggg tccgcttatc ggtagctaca caggcccctc tgattgacgt
      241 tggtgtagcc tcccgtagtc ttcctgggcc cctgggaggt acatgtcccc cagcattggt
      301 gtaagagctt cagccaagag ttacacataa aggcaatgtt gtgttgcagt ccacagactg
      361 caaagtctgc tccaggatga aagccactca gtgttggcaa atgtgcacat ccatttataa
      421 ggatgtcaac tacagtcaga gaaccccttt gtgtttggtc cccccccgtg tcacatgtgg
      481 aacagggccc agttggcaag ttgtaccaac caactgaagg gattacatgc actgccccgt
      541 gaccaataca aaacaaaagc gctcctcgta ccagcgaaga aggggcagag atgccgtagt
      601 caggtttagt tcgtccggcg gcgggggatc tggtggatct ttcctgtata attttctatt
      661 ttcatgcttc atcctcagta agcacagcaa gcatatgcag ttagcagaca ttttctttgc
      721 acactcaggc cattgtttgc agtacattgc atcaacacca ggatttaagg aagaagcaaa
      781 tacctcagtt gcatcccaga agcctccaaa gtcaggttga tgagcatatt ttactccatc
      841 ttccattttc ttgtacagag tattcatttt cttcattttt tcttcatctc ctcctttatc
      901 aggatgaaac tccttgcatt tttttaaata tgcctttctc atcagaggaa tattccccca
      961 ggcactcctt tcaagaccta gaaggtccat tagctgcaaa gattcctctc tgtttaaaac
     1021 tttatccatc tttgcaaagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
     1081 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     1141 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     1201 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     1261 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     1321 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     1381 gctggttctt tccgcctcag aaggtaccta accaagttcc tctttcagag gttatttcag
     1441 gccatggtgc tgcgccggct gtcacgccag gcctccgtta aggttcgtag gtcatggact
     1501 gaaagtaaaa aaacagctca acgccttttt gtgtttgttt tagagctttt gctgcaattt
     1561 tgtgaagggg aagatactgt tgacgggaaa cgcaaaaaac cagaaaggtt aactgaaaaa
     1621 ccagaaagtt aactggtaag tttagtcttt ttgtctttta tttcaggtcc atgggtgctg
     1681 ctttaacact gttgggggac ctaattgcta ctgtgtctga agctgctgct gctactggat
     1741 tttcagtagc tgaaattgct gctggagagg ccgctgctgc aattgaagtg caacttgcat
     1801 ctgttgctac tgttgaaggc ctaacaacct ctgaggcaat tgctgctata ggcctcactc
     1861 cacaggccta tgctgtgata tctggggctc ctgctgctat agctggattt gcagctttac
     1921 tgcaaactgt gactggtgtg agcgctgttg ctcaagtggg gtatagattt tttagtgact
     1981 gggatcacaa agtttctact gttggtttat atcaacaacc aggaatggct gtagatttgt
     2041 ataggccaga tgattactat gatattttat ttcctggagt acaaaccttt gttcacagtg
     2101 ttcagtatct tgaccccaga cattggggtc caacactttt taatgccatt tctcaagctt
     2161 tttggcgtgt aatacaaaat gacattccta ggctcacctc acaggagctt gaaagaagaa
     2221 cccaaagata tttaagggac agtttggcaa ggtttttaga ggaaactact tggacagtaa
     2281 ttaatgctcc tgttaattgg tataactctt tacaagatta ctactctact ttgtctccca
     2341 ttaggcctac aatggtgaga caagtagcca acagggaagg gttgcaaata tcatttgggc
     2401 acacctatga taatattgat gaagcagaca gtattcagca agtaactgag aggtgggaag
     2461 ctcaaagcca aagtcctaat gtgcagtcag gtgaatttat tgaaaaattt gaggctcctg
     2521 gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc tttacttcta ggcctgtacg
     2581 gaagtgttac ttctgctcta aaagctccgg atcagcttga tacaagaact actgatttca
     2641 acttctttgg cttaattctc tcggaaacga tgaaatatac aagttatatc ttggcttttc
     2701 agctctgcat cgttttgggt tctcttggct gttactgcca ggacccatat gtaaaagaag
     2761 cagaaaacct taagaaatat tttaatgcag gtcattcaga tgtagcggat aatggaactc
     2821 ttttcttagg cattttgaag aattggaaag aggagagtga cagaaaaata atgcagagcc
     2881 aaattgtctc cttttacttc aaacttttta aaaactttaa agatgaccag agcatccaaa
     2941 agagtgtgga gaccatcaag gaagacatga atgtcaagtt tttcaatagc aacaaaaaga
     3001 aacgagatga cttcgaaaag ctgactaatt attcggtaac tgacttgaat gtccaacgca
     3061 aagcaataca tgaactcatc caagtgatgg ctgaactgtc gccagcagct aaaacaggga
     3121 agcgaaaaag gagtcagatg ctgtttcgag gtcgaagagc atcccagtaa tggttgtcct
     3181 gcctgcaata tttgaatttt aaatctaaat ctatttatta atatttaaca ttatttatat
     3241 ggggaatata tttttagact catcaatcaa ataagtattt ataatagcaa cttttgtgta
     3301 atgaaaatga atatctatta atatatgtat tatttataat tcctatatcc tgtgactgtc
     3361 tcacttaatc ctttgttttc tgactaatta ggcaaggcta tgtgattaca aggctttatc
     3421 tcaggggcca actaggcagc caacctaagc aagatccaga catgataaga tacattgatg
     3481 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3541 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     3601 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     3661 acctctacaa atgtggtatg gctgattatg atcctctaga gtcgacctgc agcccaagct
     3721 tggcactggc gccagaaatc cgcgcggtgg tttttggggg tcgggggtgt ttggcagcca
     3781 cagacgcccg gtgttcgtgt cgcgccagta catgcggtcc atgcccaggc catccaaaaa
     3841 ccatgggtct gtctgctcag tccagtcgtg gacctgaccc cacgcaacgc ccaaaagaat
     3901 aacccccacg aaccataaac cattccccat gggggacccc gtccctaacc cacggggccc
     3961 gtggctatgg cgggcttgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac
     4021 ttgggggttg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg
     4081 gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc
     4141 caacacccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
     4201 attgtctcct tccgtgtttc agttagcctc ccccatctcc cgatccccac gagtgctggg
     4261 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     4321 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     4381 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     4441 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     4501 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     4561 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     4621 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     4681 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     4741 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     4801 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     4861 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     4921 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     4981 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     5041 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     5101 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     5161 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     5221 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc ggggatcttg
     5281 cggcacgctg ttgacgctgt taagcgggtc gctgcagggt cgctcggtgt tcgaggccac
     5341 acgcgtcacc ttaatatgcg aagtggacct gggaccgcgc cgccccgact gcatctgcgt
     5401 gttcgaattc gccaatgaca agacgctggg cggggtttgt gtcatcatag aactaaagac
     5461 atgcaaatat atttcttccg gggacaccgc cagcaaacgc gagcaacggg ccacggggat
     5521 gaagcagccc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     5581 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     5641 gccatctcca gcagccgcac gcggcgcatc tcggggccga cgcgctgggc tacgtcttgc
     5701 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     5761 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     5821 gacagcttca aggatcgctc gcggctctta ccagcgccag caaaaggcca ggaaccgtaa
     5881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6301 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6421 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6481 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6661 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6721 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6781 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6841 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6901 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6961 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7021 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7081 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7141 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7201 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7261 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     7321 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7381 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7441 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7501 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7561 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7621 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     7681 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//
