LOCUS       V002939                 8459 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002939
VERSION     V002939
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8459)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8459)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..316
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             349..870
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          875..940
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1070..1090
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2844..2978
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    2982..3014
                     /label="lacO"
                     /note="lacO"
     misc_feature    complement(3058..3995)
                     /label="intron"
                     /note="intron"
     CDS             complement(4002..4187)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4231..4588
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4602..5123
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             5323..5343
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    6398..6538
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6724..7312)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7486..8343)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8344..8448)
                     /label="AmpR promoter"
ORIGIN
        1 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
       61 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
      121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
      181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      301 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt
      361 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg
      421 gggaatattc ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat
      481 aaaggaggag atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat
      541 ggagtaaaat atgctcatca acctgacttt ggaggcttct gggatgcaac tgaggtattt
      601 gcttcttcct taaatcctgg tgttgatgca atgtactgca aacaatggcc tgagtgtgca
      661 aagaaaatgt ctgctaactg catatgcttg ctgtgcttac tgaggatgaa gcatgaaaat
      721 agaaaattat acaggaaaga tccacttgtg tgggttgatt gctactgctt cgattgcttt
      781 agaatgtggt ttggacttga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      841 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      901 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      961 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     1021 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     1081 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     1141 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     1201 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     1261 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     1321 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     1381 atatttgatg tatagtgcct tgactagaga tccattttct gttattgagg aaagtttgcc
     1441 aggtgggtta aaggagcatg attttaatcc agaagaagca gaggaaacta aacaagtgtc
     1501 ctggaagctt gtaacagagt atgcaatgga aacaaaatgt gatgatgtgt tgttattgct
     1561 tgggatgtac ttggaatttc agtacagttt tgaaatgtgt ttaaaatgta ttaaaaaaga
     1621 acagcccagc cactataagt accatgaaaa gcattatgca aatgctgcta tatttgctga
     1681 cagcaaaaac caaaaaacca tatgccaaca ggctgttgat actgttttag ctaaaaagcg
     1741 ggttgatagc ctacaattaa ctagagaaca aatgttaaca aacagattta atgatctttt
     1801 ggataggatg gatataatgt ttggttctac aggctctgct gacatagaag aatggatggc
     1861 tggagttgct tggctacact gtttgttgcc caaaatggat tcagtggtgt atgacttttt
     1921 aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg ctgtttaaag gaccaattga
     1981 tagtggtaaa actacattag cagctgcttt gcttgaatta tgtgggggga aagctttaaa
     2041 tgttaatttg cccttggaca ggctgaactt tgagctagga gtagctattg accagttttt
     2101 agtagttttt gaggatgtaa agggcactgg aggggagtcc agagatttgc cttcaggtca
     2161 gggaattaat aacctggaca atttaaggga ttatttggat ggcagtgtta aggtaaactt
     2221 agaaaagaaa cacctaaata aaagaactca aatatttccc cctggaatag tcaccatgaa
     2281 tgagtacagt gtgcctaaaa cactgcaggc cagatttgta aaacaaatag attttaggcc
     2341 caaagattat ttaaagcatt gcctggaacg cagtgagttt ttgttagaaa agagaataat
     2401 tcaaagtggc attgctttgc ttcttatgtt aatttggtac agacctgtgg ctgagtttgc
     2461 tcaaagtatt cagagcagaa ttgtggagtg gaaagagaga ttggacaaag agtttagttt
     2521 gtcagtgtat caaaaaatga agtttaatgt ggctatggga attggagttt tagattggct
     2581 aagaaacagt gatgatgatg atgaagacag ccaggaaaat gctgataaaa atgaagatgg
     2641 tggggagaag aacatggaag actcagggca tgaaacaggc attgattcac agtcccaagg
     2701 ctcatttcag gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat
     2761 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2821 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2881 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2941 tgtccaaact catcaatgta tcttatcatg tctggatccg gaattctgag cggataacaa
     3001 tttcacacag aattccggat ccggagcttt tagagcagaa gtaacacttc cgtacaggcc
     3061 tagaagtaaa ggcaacatcc actgaggagc agttctttga tttgcaccac caggagcctc
     3121 aaatttttca ataaattcac ctgactgcac attaggactt tggctttgag cttcccacct
     3181 ctcagttact tgctgaatac tgtctgcttc atcaatatta tcataggtgt gcccaaatga
     3241 tatttgcaac ccttccctgt tggctacttg tctcaccatt gtaggcctaa tgggagacaa
     3301 agtagagtag taatcttgta aagagttata ccaattaaca ggagcattaa ttactgtcca
     3361 agtagtttcc tctaaaaacc ttgccaaact gtcccttaaa tatctttggg ttcttctttc
     3421 aagctcctgt gaggtgagcc taggaatgtc attttgtatt acacgccaaa aagcttgaga
     3481 aatggcatta aaaagtgttg gaccccaatg tctggggtca agatactgaa cactgtgaac
     3541 aaaggtttgt actccaggaa ataaaatatc atagtaatca tctggcctat acaaatctac
     3601 agccattcct ggttgttgat ataaaccaac agtagaaact ttgtgatccc agtcactaaa
     3661 aaatctatac cccacttgag caacagcgct cacaccagtc acagtttgca gtaaagctgc
     3721 aaatccagct atagcagcag gagccccaga tatcacagca taggcctgtg gagtgaggcc
     3781 tatagcagca attgcctcag aggttgttag gccttcaaca gtagcaacag atgcaagttg
     3841 cacttcaatt gcagcagcgg cctctccagc agcaatttca gctactgaaa atccagtagc
     3901 agcagcagct tcagacacag tagcaattag gtcccccaac agtgttaaag cagcacccat
     3961 ggacctgaaa taaaagacaa aaagactaaa cttaccagtt aactttctgg tttttcagtt
     4021 aacctttctg gttttttgcg tttcccgtca acagtatctt ccccttcaca aaattgcagc
     4081 aaaagctcta aaacaaacac aaaaaggcgt tgagctgttt ttttactttc agtccatgac
     4141 ctacgaacct taacggaggc ctggcgtgac agccggcgca gcaccatggc ctgaaataac
     4201 ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt
     4261 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4321 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4381 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4441 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4501 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4561 cttttttgga ggcctaggct tttgcaaaaa gctttgcaaa gatggataaa gttttaaaca
     4621 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtgcc tgggggaata
     4681 ttcctctgat gagaaaggca tatttaaaaa aatgcaagga gtttcatcct gataaaggag
     4741 gagatgaaga aaaaatgaag aaaatgaata ctctgtacaa gaaaatggaa gatggagtaa
     4801 aatatgctca tcaacctgac tttggaggct tctgggatgc aactgaggta tttgcttctt
     4861 ccttaaatcc tggtgttgat gcaatgtact gcaaacaatg gcctgagtgt gcaaagaaaa
     4921 tgtctgctaa ctgcatatgc ttgctgtgct tactgaggat gaagcatgaa aatagaaaat
     4981 tatacaggaa agatccactt gtgtgggttg attgctactg cttcgattgc tttagaatgt
     5041 ggtttggact tgatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     5101 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     5161 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     5221 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     5281 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     5341 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     5401 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     5461 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     5521 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     5581 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     5641 atgtatagtg ccttgactag agatccattt tctgttattg aggaaagttt gccaggtggg
     5701 ttaaaggagc atgattttaa tccagaagaa gcagaggaaa ctaaacaagt gtcctggaag
     5761 cttgtaacag agtatgcaat ggaaacaaaa tgtgatgatg tgttgttatt gcttgggatg
     5821 tacttggaat ttcagtacag ttttgaaatg tgtttaaaat gtattaaaaa agaacagccc
     5881 agccactata agtaccatga aaagcattat gcaaatgctg ctatatttgc tgacagcaaa
     5941 aaccaaaaaa ccatatgcca acaggctgtt gatactgttt tagctaaaaa gcgggttgat
     6001 agcctacaat taactagaga acaaatgtta acaaacagat ttaatgatct tttggatagg
     6061 atggatataa tgtttggttc tacaggctct gctgacatag aagaatggat ggctggagtt
     6121 gcttggctac actgtttgtt gcccaaaatg gattcagtgg tgtatgactt tttaaaatgc
     6181 atggtgtaca acattcctaa aaaaagatac tggctgttta aaggaccaat tgatagtggt
     6241 aaaactacat tagcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     6301 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     6361 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     6421 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     6481 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     6541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6901 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6961 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7021 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7081 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7141 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     7201 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7261 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7321 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7381 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7441 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7501 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7561 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7621 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7681 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7741 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
     7801 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7861 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7921 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7981 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8041 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     8101 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8161 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8221 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8281 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8341 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8401 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttcccc
//
