LOCUS       V002940                 8424 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002940
VERSION     V002940
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8424)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8424)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        139..335
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     misc_feature    349..877
                     /label="SV40 small t-antigen"
                     /note="SV40 small t-antigen"
     misc_feature    349..594
                     /label="SV40 large T-antigen"
                     /note="SV40 large T-antigen"
     intron          879..944
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1074..1094
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2848..2982
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3019..3956)
                     /label="intron"
                     /note="intron"
     CDS             complement(3963..4148)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4196..4553
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4567..5088
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             5288..5308
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    6363..6503
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6689..7277)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7451..8308)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8309..8413)
                     /label="AmpR promoter"
ORIGIN
        1 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
       61 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
      121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
      181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      301 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt
      361 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg
      421 gggaatattc ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat
      481 aaaggaggag atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat
      541 ggagtaaaat atgctcatca acctgacttt ggaggcttct gggatgcaac tgaggtattt
      601 gcttcttcct taaatcctgg tgttgatgca atgtactgca aacaatggcc tgagtgtgca
      661 aagaaaatgt ctgctaactg catatgcttg ctgtgcttac tgaggatgaa gcatgaaaat
      721 agaaaattat acaggaaaga tccaccagat ctggtgggtt gattgctact gcttcgattg
      781 ctttagaatg tggtttggac ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat
      841 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
      901 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
      961 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     1021 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     1081 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     1141 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     1201 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     1261 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     1321 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     1381 aggaatattt gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt
     1441 tgccaggtgg gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag
     1501 tgtcctggaa gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat
     1561 tgcttgggat gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa
     1621 aagaacagcc cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg
     1681 ctgacagcaa aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa
     1741 agcgggttga tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc
     1801 ttttggatag gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga
     1861 tggctggagt tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact
     1921 ttttaaaatg catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa
     1981 ttgatagtgg taaaactaca ttagcagctg ctttgcttga attatgtggg gggaaagctt
     2041 taaatgttaa tttgcccttg gacaggctga actttgagct aggagtagct attgaccagt
     2101 ttttagtagt ttttgaggat gtaaagggca ctggagggga gtccagagat ttgccttcag
     2161 gtcagggaat taataacctg gacaatttaa gggattattt ggatggcagt gttaaggtaa
     2221 acttagaaaa gaaacaccta aataaaagaa ctcaaatatt tccccctgga atagtcacca
     2281 tgaatgagta cagtgtgcct aaaacactgc aggccagatt tgtaaaacaa atagatttta
     2341 ggcccaaaga ttatttaaag cattgcctgg aacgcagtga gtttttgtta gaaaagagaa
     2401 taattcaaag tggcattgct ttgcttctta tgttaatttg gtacagacct gtggctgagt
     2461 ttgctcaaag tattcagagc agaattgtgg agtggaaaga gagattggac aaagagttta
     2521 gtttgtcagt gtatcaaaaa atgaagttta atgtggctat gggaattgga gttttagatt
     2581 ggctaagaaa cagtgatgat gatgatgaag acagccagga aaatgctgat aaaaatgaag
     2641 atggtgggga gaagaacatg gaagactcag ggcatgaaac aggcattgat tcacagtccc
     2701 aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc
     2761 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2821 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     2881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2941 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccggagctt ttagagcaga
     3001 agtaacactt ccgtacaggc ctagaagtaa aggcaacatc cactgaggag cagttctttg
     3061 atttgcacca ccaggagcct caaatttttc aataaattca cctgactgca cattaggact
     3121 ttggctttga gcttcccacc tctcagttac ttgctgaata ctgtctgctt catcaatatt
     3181 atcataggtg tgcccaaatg atatttgcaa cccttccctg ttggctactt gtctcaccat
     3241 tgtaggccta atgggagaca aagtagagta gtaatcttgt aaagagttat accaattaac
     3301 aggagcatta attactgtcc aagtagtttc ctctaaaaac cttgccaaac tgtcccttaa
     3361 atatctttgg gttcttcttt caagctcctg tgaggtgagc ctaggaatgt cattttgtat
     3421 tacacgccaa aaagcttgag aaatggcatt aaaaagtgtt ggaccccaat gtctggggtc
     3481 aagatactga acactgtgaa caaaggtttg tactccagga aataaaatat catagtaatc
     3541 atctggccta tacaaatcta cagccattcc tggttgttga tataaaccaa cagtagaaac
     3601 tttgtgatcc cagtcactaa aaaatctata ccccacttga gcaacagcgc tcacaccagt
     3661 cacagtttgc agtaaagctg caaatccagc tatagcagca ggagccccag atatcacagc
     3721 ataggcctgt ggagtgaggc ctatagcagc aattgcctca gaggttgtta ggccttcaac
     3781 agtagcaaca gatgcaagtt gcacttcaat tgcagcagcg gcctctccag cagcaatttc
     3841 agctactgaa aatccagtag cagcagcagc ttcagacaca gtagcaatta ggtcccccaa
     3901 cagtgttaaa gcagcaccca tggacctgaa ataaaagaca aaaagactaa acttaccagt
     3961 taactttctg gtttttcagt taacctttct ggttttttgc gtttcccgtc aacagtatct
     4021 tccccttcac aaaattgcag caaaagctct aaaacaaaca caaaaaggcg ttgagctgtt
     4081 tttttacttt cagtccatga cctacgaacc ttaacggagg cctggcgtga cagccggcgc
     4141 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttagggaatt cccttctgag
     4201 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4261 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4321 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4381 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4441 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4501 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttt
     4561 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     4621 cttgaaagga gtgcctgggg gaatattcct ctgatgagaa aggcatattt aaaaaaatgc
     4681 aaggagtttc atcctgataa aggaggagat gaagaaaaaa tgaagaaaat gaatactctg
     4741 tacaagaaaa tggaagatgg agtaaaatat gctcatcaac ctgactttgg aggcttctgg
     4801 gatgcaactg aggtatttgc ttcttcctta aatcctggtg ttgatgcaat gtactgcaaa
     4861 caatggcctg agtgtgcaaa gaaaatgtct gctaactgca tatgcttgct gtgcttactg
     4921 aggatgaagc atgaaaatag aaaattatac aggaaagatc cacttgtgtg ggttgattgc
     4981 tactgcttcg attgctttag aatgtggttt ggacttgatc tttgtgaagg aaccttactt
     5041 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     5101 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     5161 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     5221 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     5281 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     5341 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     5401 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     5461 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     5521 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     5581 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc cattttctgt
     5641 tattgaggaa agtttgccag gtgggttaaa ggagcatgat tttaatccag aagaagcaga
     5701 ggaaactaaa caagtgtcct ggaagcttgt aacagagtat gcaatggaaa caaaatgtga
     5761 tgatgtgttg ttattgcttg ggatgtactt ggaatttcag tacagttttg aaatgtgttt
     5821 aaaatgtatt aaaaaagaac agcccagcca ctataagtac catgaaaagc attatgcaaa
     5881 tgctgctata tttgctgaca gcaaaaacca aaaaaccata tgccaacagg ctgttgatac
     5941 tgttttagct aaaaagcggg ttgatagcct acaattaact agagaacaaa tgttaacaaa
     6001 cagatttaat gatcttttgg ataggatgga tataatgttt ggttctacag gctctgctga
     6061 catagaagaa tggatggctg gagttgcttg gctacactgt ttgttgccca aaatggattc
     6121 agtggtgtat gactttttaa aatgcatggt gtacaacatt cctaaaaaaa gatactggct
     6181 gtttaaagga ccaattgata gtggtaaaac tacattagca gctgcctcgc gcgtttcggt
     6241 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6301 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6361 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     6421 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6481 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7741 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8041 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     8101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8401 taggggttcc gcgcacattt cccc
//
