LOCUS       V002941                 8424 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002941
VERSION     V002941
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8424)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8424)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..316
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             349..870
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             1070..1090
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2844..2978
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3015..3952)
                     /label="intron"
                     /note="intron"
     CDS             complement(3959..4144)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4192..4549
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    4563..4808
                     /label="SV40 large T-antigen"
                     /note="SV40 large T-antigen"
     misc_feature    4567..5091
                     /label="SV40 small t-antigen"
                     /note="SV40 small t-antigen"
     misc_feature    4809..5158
                     /label="intron"
                     /note="intron"
     intron          5093..5158
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5288..5308
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    6363..6503
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6689..7277)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7451..8308)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8309..8413)
                     /label="AmpR promoter"
ORIGIN
        1 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
       61 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
      121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
      181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      301 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt
      361 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg
      421 gggaatattc ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat
      481 aaaggaggag atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat
      541 ggagtaaaat atgctcatca acctgacttt ggaggcttct gggatgcaac tgaggtattt
      601 gcttcttcct taaatcctgg tgttgatgca atgtactgca aacaatggcc tgagtgtgca
      661 aagaaaatgt ctgctaactg catatgcttg ctgtgcttac tgaggatgaa gcatgaaaat
      721 agaaaattat acaggaaaga tccacttgtg tgggttgatt gctactgctt cgattgcttt
      781 agaatgtggt ttggacttga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      841 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      901 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      961 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     1021 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     1081 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     1141 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     1201 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     1261 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     1321 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     1381 atatttgatg tatagtgcct tgactagaga tccattttct gttattgagg aaagtttgcc
     1441 aggtgggtta aaggagcatg attttaatcc agaagaagca gaggaaacta aacaagtgtc
     1501 ctggaagctt gtaacagagt atgcaatgga aacaaaatgt gatgatgtgt tgttattgct
     1561 tgggatgtac ttggaatttc agtacagttt tgaaatgtgt ttaaaatgta ttaaaaaaga
     1621 acagcccagc cactataagt accatgaaaa gcattatgca aatgctgcta tatttgctga
     1681 cagcaaaaac caaaaaacca tatgccaaca ggctgttgat actgttttag ctaaaaagcg
     1741 ggttgatagc ctacaattaa ctagagaaca aatgttaaca aacagattta atgatctttt
     1801 ggataggatg gatataatgt ttggttctac aggctctgct gacatagaag aatggatggc
     1861 tggagttgct tggctacact gtttgttgcc caaaatggat tcagtggtgt atgacttttt
     1921 aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg ctgtttaaag gaccaattga
     1981 tagtggtaaa actacattag cagctgcttt gcttgaatta tgtgggggga aagctttaaa
     2041 tgttaatttg cccttggaca ggctgaactt tgagctagga gtagctattg accagttttt
     2101 agtagttttt gaggatgtaa agggcactgg aggggagtcc agagatttgc cttcaggtca
     2161 gggaattaat aacctggaca atttaaggga ttatttggat ggcagtgtta aggtaaactt
     2221 agaaaagaaa cacctaaata aaagaactca aatatttccc cctggaatag tcaccatgaa
     2281 tgagtacagt gtgcctaaaa cactgcaggc cagatttgta aaacaaatag attttaggcc
     2341 caaagattat ttaaagcatt gcctggaacg cagtgagttt ttgttagaaa agagaataat
     2401 tcaaagtggc attgctttgc ttcttatgtt aatttggtac agacctgtgg ctgagtttgc
     2461 tcaaagtatt cagagcagaa ttgtggagtg gaaagagaga ttggacaaag agtttagttt
     2521 gtcagtgtat caaaaaatga agtttaatgt ggctatggga attggagttt tagattggct
     2581 aagaaacagt gatgatgatg atgaagacag ccaggaaaat gctgataaaa atgaagatgg
     2641 tggggagaag aacatggaag actcagggca tgaaacaggc attgattcac agtcccaagg
     2701 ctcatttcag gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat
     2761 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2821 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2881 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2941 tgtccaaact catcaatgta tcttatcatg tctggatccg gagcttttag agcagaagta
     3001 acacttccgt acaggcctag aagtaaaggc aacatccact gaggagcagt tctttgattt
     3061 gcaccaccag gagcctcaaa tttttcaata aattcacctg actgcacatt aggactttgg
     3121 ctttgagctt cccacctctc agttacttgc tgaatactgt ctgcttcatc aatattatca
     3181 taggtgtgcc caaatgatat ttgcaaccct tccctgttgg ctacttgtct caccattgta
     3241 ggcctaatgg gagacaaagt agagtagtaa tcttgtaaag agttatacca attaacagga
     3301 gcattaatta ctgtccaagt agtttcctct aaaaaccttg ccaaactgtc ccttaaatat
     3361 ctttgggttc ttctttcaag ctcctgtgag gtgagcctag gaatgtcatt ttgtattaca
     3421 cgccaaaaag cttgagaaat ggcattaaaa agtgttggac cccaatgtct ggggtcaaga
     3481 tactgaacac tgtgaacaaa ggtttgtact ccaggaaata aaatatcata gtaatcatct
     3541 ggcctataca aatctacagc cattcctggt tgttgatata aaccaacagt agaaactttg
     3601 tgatcccagt cactaaaaaa tctatacccc acttgagcaa cagcgctcac accagtcaca
     3661 gtttgcagta aagctgcaaa tccagctata gcagcaggag ccccagatat cacagcatag
     3721 gcctgtggag tgaggcctat agcagcaatt gcctcagagg ttgttaggcc ttcaacagta
     3781 gcaacagatg caagttgcac ttcaattgca gcagcggcct ctccagcagc aatttcagct
     3841 actgaaaatc cagtagcagc agcagcttca gacacagtag caattaggtc ccccaacagt
     3901 gttaaagcag cacccatgga cctgaaataa aagacaaaaa gactaaactt accagttaac
     3961 tttctggttt ttcagttaac ctttctggtt ttttgcgttt cccgtcaaca gtatcttccc
     4021 cttcacaaaa ttgcagcaaa agctctaaaa caaacacaaa aaggcgttga gctgtttttt
     4081 tactttcagt ccatgaccta cgaaccttaa cggaggcctg gcgtgacagc cggcgcagca
     4141 ccatggcctg aaataacctc tgaaagagga acttggttag ggaattccct tctgaggcgg
     4201 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4261 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4321 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4381 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4441 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4501 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttgcaa
     4561 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     4621 aaaggagtgc ctgggggaat attcctctga tgagaaaggc atatttaaaa aaatgcaagg
     4681 agtttcatcc tgataaagga ggagatgaag aaaaaatgaa gaaaatgaat actctgtaca
     4741 agaaaatgga agatggagta aaatatgctc atcaacctga ctttggaggc ttctgggatg
     4801 caactgaggt atttgcttct tccttaaatc ctggtgttga tgcaatgtac tgcaaacaat
     4861 ggcctgagtg tgcaaagaaa atgtctgcta actgcatatg cttgctgtgc ttactgagga
     4921 tgaagcatga aaatagaaaa ttatacagga aagatccacc agatctggtg ggttgattgc
     4981 tactgcttcg attgctttag aatgtggttt ggacttgatc tttgtgaagg aaccttactt
     5041 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     5101 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     5161 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     5221 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     5281 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     5341 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     5401 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     5461 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     5521 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     5581 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc cattttctgt
     5641 tattgaggaa agtttgccag gtgggttaaa ggagcatgat tttaatccag aagaagcaga
     5701 ggaaactaaa caagtgtcct ggaagcttgt aacagagtat gcaatggaaa caaaatgtga
     5761 tgatgtgttg ttattgcttg ggatgtactt ggaatttcag tacagttttg aaatgtgttt
     5821 aaaatgtatt aaaaaagaac agcccagcca ctataagtac catgaaaagc attatgcaaa
     5881 tgctgctata tttgctgaca gcaaaaacca aaaaaccata tgccaacagg ctgttgatac
     5941 tgttttagct aaaaagcggg ttgatagcct acaattaact agagaacaaa tgttaacaaa
     6001 cagatttaat gatcttttgg ataggatgga tataatgttt ggttctacag gctctgctga
     6061 catagaagaa tggatggctg gagttgcttg gctacactgt ttgttgccca aaatggattc
     6121 agtggtgtat gactttttaa aatgcatggt gtacaacatt cctaaaaaaa gatactggct
     6181 gtttaaagga ccaattgata gtggtaaaac tacattagca gctgcctcgc gcgtttcggt
     6241 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6301 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6361 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     6421 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6481 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7741 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8041 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     8101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8401 taggggttcc gcgcacattt cccc
//
