LOCUS       V002942                 8420 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002942
VERSION     V002942
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..316
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             349..870
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          875..940
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1070..1090
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2844..2978
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3015..3952)
                     /label="intron"
                     /note="intron"
     CDS             complement(3959..4144)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4192..4549
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4563..5084
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             5284..5304
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    6359..6499
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6685..7273)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7447..8304)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8305..8409)
                     /label="AmpR promoter"
ORIGIN
        1 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
       61 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
      121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
      181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      301 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt
      361 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg
      421 gggaatattc ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat
      481 aaaggaggag atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat
      541 ggagtaaaat atgctcatca acctgacttt ggaggcttct gggatgcaac tgaggtattt
      601 gcttcttcct taaatcctgg tgttgatgca atgtactgca aacaatggcc tgagtgtgca
      661 aagaaaatgt ctgctaactg catatgcttg ctgtgcttac tgaggatgaa gcatgaaaat
      721 agaaaattat acaggaaaga tccacttgtg tgggttgatt gctactgctt cgattgcttt
      781 agaatgtggt ttggacttga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      841 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      901 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      961 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     1021 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     1081 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     1141 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     1201 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     1261 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     1321 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     1381 atatttgatg tatagtgcct tgactagaga tccattttct gttattgagg aaagtttgcc
     1441 aggtgggtta aaggagcatg attttaatcc agaagaagca gaggaaacta aacaagtgtc
     1501 ctggaagctt gtaacagagt atgcaatgga aacaaaatgt gatgatgtgt tgttattgct
     1561 tgggatgtac ttggaatttc agtacagttt tgaaatgtgt ttaaaatgta ttaaaaaaga
     1621 acagcccagc cactataagt accatgaaaa gcattatgca aatgctgcta tatttgctga
     1681 cagcaaaaac caaaaaacca tatgccaaca ggctgttgat actgttttag ctaaaaagcg
     1741 ggttgatagc ctacaattaa ctagagaaca aatgttaaca aacagattta atgatctttt
     1801 ggataggatg gatataatgt ttggttctac aggctctgct gacatagaag aatggatggc
     1861 tggagttgct tggctacact gtttgttgcc caaaatggat tcagtggtgt atgacttttt
     1921 aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg ctgtttaaag gaccaattga
     1981 tagtggtaaa actacattag cagctgcttt gcttgaatta tgtgggggga aagctttaaa
     2041 tgttaatttg cccttggaca ggctgaactt tgagctagga gtagctattg accagttttt
     2101 agtagttttt gaggatgtaa agggcactgg aggggagtcc agagatttgc cttcaggtca
     2161 gggaattaat aacctggaca atttaaggga ttatttggat ggcagtgtta aggtaaactt
     2221 agaaaagaaa cacctaaata aaagaactca aatatttccc cctggaatag tcaccatgaa
     2281 tgagtacagt gtgcctaaaa cactgcaggc cagatttgta aaacaaatag attttaggcc
     2341 caaagattat ttaaagcatt gcctggaacg cagtgagttt ttgttagaaa agagaataat
     2401 tcaaagtggc attgctttgc ttcttatgtt aatttggtac agacctgtgg ctgagtttgc
     2461 tcaaagtatt cagagcagaa ttgtggagtg gaaagagaga ttggacaaag agtttagttt
     2521 gtcagtgtat caaaaaatga agtttaatgt ggctatggga attggagttt tagattggct
     2581 aagaaacagt gatgatgatg atgaagacag ccaggaaaat gctgataaaa atgaagatgg
     2641 tggggagaag aacatggaag actcagggca tgaaacaggc attgattcac agtcccaagg
     2701 ctcatttcag gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat
     2761 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2821 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2881 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2941 tgtccaaact catcaatgta tcttatcatg tctggatccg gagcttttag agcagaagta
     3001 acacttccgt acaggcctag aagtaaaggc aacatccact gaggagcagt tctttgattt
     3061 gcaccaccag gagcctcaaa tttttcaata aattcacctg actgcacatt aggactttgg
     3121 ctttgagctt cccacctctc agttacttgc tgaatactgt ctgcttcatc aatattatca
     3181 taggtgtgcc caaatgatat ttgcaaccct tccctgttgg ctacttgtct caccattgta
     3241 ggcctaatgg gagacaaagt agagtagtaa tcttgtaaag agttatacca attaacagga
     3301 gcattaatta ctgtccaagt agtttcctct aaaaaccttg ccaaactgtc ccttaaatat
     3361 ctttgggttc ttctttcaag ctcctgtgag gtgagcctag gaatgtcatt ttgtattaca
     3421 cgccaaaaag cttgagaaat ggcattaaaa agtgttggac cccaatgtct ggggtcaaga
     3481 tactgaacac tgtgaacaaa ggtttgtact ccaggaaata aaatatcata gtaatcatct
     3541 ggcctataca aatctacagc cattcctggt tgttgatata aaccaacagt agaaactttg
     3601 tgatcccagt cactaaaaaa tctatacccc acttgagcaa cagcgctcac accagtcaca
     3661 gtttgcagta aagctgcaaa tccagctata gcagcaggag ccccagatat cacagcatag
     3721 gcctgtggag tgaggcctat agcagcaatt gcctcagagg ttgttaggcc ttcaacagta
     3781 gcaacagatg caagttgcac ttcaattgca gcagcggcct ctccagcagc aatttcagct
     3841 actgaaaatc cagtagcagc agcagcttca gacacagtag caattaggtc ccccaacagt
     3901 gttaaagcag cacccatgga cctgaaataa aagacaaaaa gactaaactt accagttaac
     3961 tttctggttt ttcagttaac ctttctggtt ttttgcgttt cccgtcaaca gtatcttccc
     4021 cttcacaaaa ttgcagcaaa agctctaaaa caaacacaaa aaggcgttga gctgtttttt
     4081 tactttcagt ccatgaccta cgaaccttaa cggaggcctg gcgtgacagc cggcgcagca
     4141 ccatggcctg aaataacctc tgaaagagga acttggttag ggaattccct tctgaggcgg
     4201 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4261 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4321 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4381 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4441 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4501 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttgcaa
     4561 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     4621 aaaggagtgc ctgggggaat attcctctga tgagaaaggc atatttaaaa aaatgcaagg
     4681 agtttcatcc tgataaagga ggagatgaag aaaaaatgaa gaaaatgaat actctgtaca
     4741 agaaaatgga agatggagta aaatatgctc atcaacctga ctttggaggc ttctgggatg
     4801 caactgaggt atttgcttct tccttaaatc ctggtgttga tgcaatgtac tgcaaacaat
     4861 ggcctgagtg tgcaaagaaa atgtctgcta actgcatatg cttgctgtgc ttactgagga
     4921 tgaagcatga aaatagaaaa ttatacagga aagatccact tgtgtgggtt gattgctact
     4981 gcttcgattg ctttagaatg tggtttggac ttgatctttg tgaaggaacc ttacttctgt
     5041 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     5101 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     5161 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     5221 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     5281 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     5341 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     5401 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     5461 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     5521 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     5581 ggggttaata aggaatattt gatgtatagt gccttgacta gagatccatt ttctgttatt
     5641 gaggaaagtt tgccaggtgg gttaaaggag catgatttta atccagaaga agcagaggaa
     5701 actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat
     5761 gtgttgttat tgcttgggat gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa
     5821 tgtattaaaa aagaacagcc cagccactat aagtaccatg aaaagcatta tgcaaatgct
     5881 gctatatttg ctgacagcaa aaaccaaaaa accatatgcc aacaggctgt tgatactgtt
     5941 ttagctaaaa agcgggttga tagcctacaa ttaactagag aacaaatgtt aacaaacaga
     6001 tttaatgatc ttttggatag gatggatata atgtttggtt ctacaggctc tgctgacata
     6061 gaagaatgga tggctggagt tgcttggcta cactgtttgt tgcccaaaat ggattcagtg
     6121 gtgtatgact ttttaaaatg catggtgtac aacattccta aaaaaagata ctggctgttt
     6181 aaaggaccaa ttgatagtgg taaaactaca ttagcagctg cctcgcgcgt ttcggtgatg
     6241 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     6301 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     6361 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     6421 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6481 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7741 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8041 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     8101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8401 ggttccgcgc acatttcccc
//
