LOCUS       V002943                 8416 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002943
VERSION     V002943
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..316
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             349..870
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          875..940
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1070..1090
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2844..2978
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3015..3952)
                     /label="intron"
                     /note="intron"
     CDS             complement(3959..4144)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4188..4545
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4559..5080
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             5280..5300
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    6355..6495
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6681..7269)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7443..8300)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8301..8405)
                     /label="AmpR promoter"
ORIGIN
        1 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
       61 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
      121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
      181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      301 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt
      361 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg
      421 gggaatattc ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat
      481 aaaggaggag atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat
      541 ggagtaaaat atgctcatca acctgacttt ggaggcttct gggatgcaac tgaggtattt
      601 gcttcttcct taaatcctgg tgttgatgca atgtactgca aacaatggcc tgagtgtgca
      661 aagaaaatgt ctgctaactg catatgcttg ctgtgcttac tgaggatgaa gcatgaaaat
      721 agaaaattat acaggaaaga tccacttgtg tgggttgatt gctactgctt cgattgcttt
      781 agaatgtggt ttggacttga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      841 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      901 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      961 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     1021 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     1081 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     1141 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     1201 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     1261 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     1321 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     1381 atatttgatg tatagtgcct tgactagaga tccattttct gttattgagg aaagtttgcc
     1441 aggtgggtta aaggagcatg attttaatcc agaagaagca gaggaaacta aacaagtgtc
     1501 ctggaagctt gtaacagagt atgcaatgga aacaaaatgt gatgatgtgt tgttattgct
     1561 tgggatgtac ttggaatttc agtacagttt tgaaatgtgt ttaaaatgta ttaaaaaaga
     1621 acagcccagc cactataagt accatgaaaa gcattatgca aatgctgcta tatttgctga
     1681 cagcaaaaac caaaaaacca tatgccaaca ggctgttgat actgttttag ctaaaaagcg
     1741 ggttgatagc ctacaattaa ctagagaaca aatgttaaca aacagattta atgatctttt
     1801 ggataggatg gatataatgt ttggttctac aggctctgct gacatagaag aatggatggc
     1861 tggagttgct tggctacact gtttgttgcc caaaatggat tcagtggtgt atgacttttt
     1921 aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg ctgtttaaag gaccaattga
     1981 tagtggtaaa actacattag cagctgcttt gcttgaatta tgtgggggga aagctttaaa
     2041 tgttaatttg cccttggaca ggctgaactt tgagctagga gtagctattg accagttttt
     2101 agtagttttt gaggatgtaa agggcactgg aggggagtcc agagatttgc cttcaggtca
     2161 gggaattaat aacctggaca atttaaggga ttatttggat ggcagtgtta aggtaaactt
     2221 agaaaagaaa cacctaaata aaagaactca aatatttccc cctggaatag tcaccatgaa
     2281 tgagtacagt gtgcctaaaa cactgcaggc cagatttgta aaacaaatag attttaggcc
     2341 caaagattat ttaaagcatt gcctggaacg cagtgagttt ttgttagaaa agagaataat
     2401 tcaaagtggc attgctttgc ttcttatgtt aatttggtac agacctgtgg ctgagtttgc
     2461 tcaaagtatt cagagcagaa ttgtggagtg gaaagagaga ttggacaaag agtttagttt
     2521 gtcagtgtat caaaaaatga agtttaatgt ggctatggga attggagttt tagattggct
     2581 aagaaacagt gatgatgatg atgaagacag ccaggaaaat gctgataaaa atgaagatgg
     2641 tggggagaag aacatggaag actcagggca tgaaacaggc attgattcac agtcccaagg
     2701 ctcatttcag gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat
     2761 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2821 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2881 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2941 tgtccaaact catcaatgta tcttatcatg tctggatccg gagcttttag agcagaagta
     3001 acacttccgt acaggcctag aagtaaaggc aacatccact gaggagcagt tctttgattt
     3061 gcaccaccag gagcctcaaa tttttcaata aattcacctg actgcacatt aggactttgg
     3121 ctttgagctt cccacctctc agttacttgc tgaatactgt ctgcttcatc aatattatca
     3181 taggtgtgcc caaatgatat ttgcaaccct tccctgttgg ctacttgtct caccattgta
     3241 ggcctaatgg gagacaaagt agagtagtaa tcttgtaaag agttatacca attaacagga
     3301 gcattaatta ctgtccaagt agtttcctct aaaaaccttg ccaaactgtc ccttaaatat
     3361 ctttgggttc ttctttcaag ctcctgtgag gtgagcctag gaatgtcatt ttgtattaca
     3421 cgccaaaaag cttgagaaat ggcattaaaa agtgttggac cccaatgtct ggggtcaaga
     3481 tactgaacac tgtgaacaaa ggtttgtact ccaggaaata aaatatcata gtaatcatct
     3541 ggcctataca aatctacagc cattcctggt tgttgatata aaccaacagt agaaactttg
     3601 tgatcccagt cactaaaaaa tctatacccc acttgagcaa cagcgctcac accagtcaca
     3661 gtttgcagta aagctgcaaa tccagctata gcagcaggag ccccagatat cacagcatag
     3721 gcctgtggag tgaggcctat agcagcaatt gcctcagagg ttgttaggcc ttcaacagta
     3781 gcaacagatg caagttgcac ttcaattgca gcagcggcct ctccagcagc aatttcagct
     3841 actgaaaatc cagtagcagc agcagcttca gacacagtag caattaggtc ccccaacagt
     3901 gttaaagcag cacccatgga cctgaaataa aagacaaaaa gactaaactt accagttaac
     3961 tttctggttt ttcagttaac ctttctggtt ttttgcgttt cccgtcaaca gtatcttccc
     4021 cttcacaaaa ttgcagcaaa agctctaaaa caaacacaaa aaggcgttga gctgtttttt
     4081 tactttcagt ccatgaccta cgaaccttaa cggaggcctg gcgtgacagc cggcgcagca
     4141 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     4201 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4261 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4321 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4381 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4441 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4501 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat
     4561 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     4621 gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt
     4681 tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa
     4741 aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct gggatgcaac
     4801 tgaggtattt gcttcttcct taaatcctgg tgttgatgca atgtactgca aacaatggcc
     4861 tgagtgtgca aagaaaatgt ctgctaactg catatgcttg ctgtgcttac tgaggatgaa
     4921 gcatgaaaat agaaaattat acaggaaaga tccacttgtg tgggttgatt gctactgctt
     4981 cgattgcttt agaatgtggt ttggacttga tctttgtgaa ggaaccttac ttctgtggtg
     5041 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     5101 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     5161 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     5221 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     5281 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     5341 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     5401 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     5461 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     5521 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     5581 ttaataagga atatttgatg tatagtgcct tgactagaga tccattttct gttattgagg
     5641 aaagtttgcc aggtgggtta aaggagcatg attttaatcc agaagaagca gaggaaacta
     5701 aacaagtgtc ctggaagctt gtaacagagt atgcaatgga aacaaaatgt gatgatgtgt
     5761 tgttattgct tgggatgtac ttggaatttc agtacagttt tgaaatgtgt ttaaaatgta
     5821 ttaaaaaaga acagcccagc cactataagt accatgaaaa gcattatgca aatgctgcta
     5881 tatttgctga cagcaaaaac caaaaaacca tatgccaaca ggctgttgat actgttttag
     5941 ctaaaaagcg ggttgatagc ctacaattaa ctagagaaca aatgttaaca aacagattta
     6001 atgatctttt ggataggatg gatataatgt ttggttctac aggctctgct gacatagaag
     6061 aatggatggc tggagttgct tggctacact gtttgttgcc caaaatggat tcagtggtgt
     6121 atgacttttt aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg ctgtttaaag
     6181 gaccaattga tagtggtaaa actacattag cagctgcctc gcgcgtttcg gtgatgacgg
     6241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     6301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     6361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     6421 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6481 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6601 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7081 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7321 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7381 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7441 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7501 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7561 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7621 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7681 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7741 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7801 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7861 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7921 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7981 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8041 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8101 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8161 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8221 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8281 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8341 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8401 ccgcgcacat ttcccc
//
