LOCUS       V002945                 9459 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002945
VERSION     V002945
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9459)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        243..439
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     misc_feature    453..981
                     /label="SV40 small t-antigen"
                     /note="SV40 small t-antigen"
     misc_feature    453..698
                     /label="SV40 large T-antigen"
                     /note="SV40 large T-antigen"
     intron          983..1048
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1178..1198
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2952..3086
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3372..3869)
                     /gene="IFNG"
                     /label="Interferon gamma"
                     /note="Interferon gamma from Homo sapiens. Accession#:
                     P01579"
     misc_feature    complement(3966..4903)
                     /label="intron"
                     /note="intron"
     CDS             complement(4910..5095)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5139..5496
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5510..6031
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6231..6251
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7306..7446
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7632..8220)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8394..9251)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9252..9356)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccacc agatctggtg ggttgattgc tactgcttcg attgctttag aatgtggttt
      901 ggacttgatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
      961 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1021 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1081 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     1141 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     1201 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     1261 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     1321 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     1381 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     1441 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     1501 tagtgccttg actagagatc cattttctgt tattgaggaa agtttgccag gtgggttaaa
     1561 ggagcatgat tttaatccag aagaagcaga ggaaactaaa caagtgtcct ggaagcttgt
     1621 aacagagtat gcaatggaaa caaaatgtga tgatgtgttg ttattgcttg ggatgtactt
     1681 ggaatttcag tacagttttg aaatgtgttt aaaatgtatt aaaaaagaac agcccagcca
     1741 ctataagtac catgaaaagc attatgcaaa tgctgctata tttgctgaca gcaaaaacca
     1801 aaaaaccata tgccaacagg ctgttgatac tgttttagct aaaaagcggg ttgatagcct
     1861 acaattaact agagaacaaa tgttaacaaa cagatttaat gatcttttgg ataggatgga
     1921 tataatgttt ggttctacag gctctgctga catagaagaa tggatggctg gagttgcttg
     1981 gctacactgt ttgttgccca aaatggattc agtggtgtat gactttttaa aatgcatggt
     2041 gtacaacatt cctaaaaaaa gatactggct gtttaaagga ccaattgata gtggtaaaac
     2101 tacattagca gctgctttgc ttgaattatg tggggggaaa gctttaaatg ttaatttgcc
     2161 cttggacagg ctgaactttg agctaggagt agctattgac cagtttttag tagtttttga
     2221 ggatgtaaag ggcactggag gggagtccag agatttgcct tcaggtcagg gaattaataa
     2281 cctggacaat ttaagggatt atttggatgg cagtgttaag gtaaacttag aaaagaaaca
     2341 cctaaataaa agaactcaaa tatttccccc tggaatagtc accatgaatg agtacagtgt
     2401 gcctaaaaca ctgcaggcca gatttgtaaa acaaatagat tttaggccca aagattattt
     2461 aaagcattgc ctggaacgca gtgagttttt gttagaaaag agaataattc aaagtggcat
     2521 tgctttgctt cttatgttaa tttggtacag acctgtggct gagtttgctc aaagtattca
     2581 gagcagaatt gtggagtgga aagagagatt ggacaaagag tttagtttgt cagtgtatca
     2641 aaaaatgaag tttaatgtgg ctatgggaat tggagtttta gattggctaa gaaacagtga
     2701 tgatgatgat gaagacagcc aggaaaatgc tgataaaaat gaagatggtg gggagaagaa
     2761 catggaagac tcagggcatg aaacaggcat tgattcacag tcccaaggct catttcaggc
     2821 ccctcagtcc tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt
     2881 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2941 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3001 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3061 tcaatgtatc ttatcatgtc tggatcttgc ttaggttggc tgcctagttg gcccctgaga
     3121 taaagccttg taatcacata gccttgccta attagtcaga aaacaaagga ttaagtgaga
     3181 cagtcacagg atataggaat tataaataat acatatatta atagatattc attttcatta
     3241 cacaaaagtt gctattataa atacttattt gattgatgag tctaaaaata tattccccat
     3301 ataaataatg ttaaatatta ataaatagat ttagatttaa aattcaaata ttgcaggcag
     3361 gacaaccatt actgggatgc tcttcgacct cgaaacagca tctgactcct ttttcgcttc
     3421 cctgttttag ctgctggcga cagttcagcc atcacttgga tgagttcatg tattgctttg
     3481 cgttggacat tcaagtcagt taccgaataa ttagtcagct tttcgaagtc atctcgtttc
     3541 tttttgttgc tattgaaaaa cttgacattc atgtcttcct tgatggtctc cacactcttt
     3601 tggatgctct ggtcatcttt aaagttttta aaaagtttga agtaaaagga gacaatttgg
     3661 ctctgcatta tttttctgtc actctcctct ttccaattct tcaaaatgcc taagaaaaga
     3721 gttccattat ccgctacatc tgaatgacct gcattaaaat atttcttaag gttttctgct
     3781 tcttttacat atgggtcctg gcagtaacag ccaagagaac ccaaaacgat gcagagctga
     3841 aaagccaaga tataacttgt atatttcatc gtttccgaga gaattaagcc aaagaagttg
     3901 aaatcagtag ttcttgtatc aagctgatcc ggagctttta gagcagaagt aacacttccg
     3961 tacaggccta gaagtaaagg caacatccac tgaggagcag ttctttgatt tgcaccacca
     4021 ggagcctcaa atttttcaat aaattcacct gactgcacat taggactttg gctttgagct
     4081 tcccacctct cagttacttg ctgaatactg tctgcttcat caatattatc ataggtgtgc
     4141 ccaaatgata tttgcaaccc ttccctgttg gctacttgtc tcaccattgt aggcctaatg
     4201 ggagacaaag tagagtagta atcttgtaaa gagttatacc aattaacagg agcattaatt
     4261 actgtccaag tagtttcctc taaaaacctt gccaaactgt cccttaaata tctttgggtt
     4321 cttctttcaa gctcctgtga ggtgagccta ggaatgtcat tttgtattac acgccaaaaa
     4381 gcttgagaaa tggcattaaa aagtgttgga ccccaatgtc tggggtcaag atactgaaca
     4441 ctgtgaacaa aggtttgtac tccaggaaat aaaatatcat agtaatcatc tggcctatac
     4501 aaatctacag ccattcctgg ttgttgatat aaaccaacag tagaaacttt gtgatcccag
     4561 tcactaaaaa atctataccc cacttgagca acagcgctca caccagtcac agtttgcagt
     4621 aaagctgcaa atccagctat agcagcagga gccccagata tcacagcata ggcctgtgga
     4681 gtgaggccta tagcagcaat tgcctcagag gttgttaggc cttcaacagt agcaacagat
     4741 gcaagttgca cttcaattgc agcagcggcc tctccagcag caatttcagc tactgaaaat
     4801 ccagtagcag cagcagcttc agacacagta gcaattaggt cccccaacag tgttaaagca
     4861 gcacccatgg acctgaaata aaagacaaaa agactaaact taccagttaa ctttctggtt
     4921 tttcagttaa cctttctggt tttttgcgtt tcccgtcaac agtatcttcc ccttcacaaa
     4981 attgcagcaa aagctctaaa acaaacacaa aaaggcgttg agctgttttt ttactttcag
     5041 tccatgacct acgaacctta acggaggcct ggcgtgacag ccggcgcagc accatggcct
     5101 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg
     5161 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5221 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5281 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5341 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5401 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5461 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttgcaaaga tggataaagt
     5521 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgcctg
     5581 ggggaatatt cctctgatga gaaaggcata tttaaaaaaa tgcaaggagt ttcatcctga
     5641 taaaggagga gatgaagaaa aaatgaagaa aatgaatact ctgtacaaga aaatggaaga
     5701 tggagtaaaa tatgctcatc aacctgactt tggaggcttc tgggatgcaa ctgaggtatt
     5761 tgcttcttcc ttaaatcctg gtgttgatgc aatgtactgc aaacaatggc ctgagtgtgc
     5821 aaagaaaatg tctgctaact gcatatgctt gctgtgctta ctgaggatga agcatgaaaa
     5881 tagaaaatta tacaggaaag atccacttgt gtgggttgat tgctactgct tcgattgctt
     5941 tagaatgtgg tttggacttg atctttgtga aggaacctta cttctgtggt gtgacataat
     6001 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     6061 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
     6121 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
     6181 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
     6241 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
     6301 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
     6361 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
     6421 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
     6481 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
     6541 aatatttgat gtatagtgcc ttgactagag atccattttc tgttattgag gaaagtttgc
     6601 caggtgggtt aaaggagcat gattttaatc cagaagaagc agaggaaact aaacaagtgt
     6661 cctggaagct tgtaacagag tatgcaatgg aaacaaaatg tgatgatgtg ttgttattgc
     6721 ttgggatgta cttggaattt cagtacagtt ttgaaatgtg tttaaaatgt attaaaaaag
     6781 aacagcccag ccactataag taccatgaaa agcattatgc aaatgctgct atatttgctg
     6841 acagcaaaaa ccaaaaaacc atatgccaac aggctgttga tactgtttta gctaaaaagc
     6901 gggttgatag cctacaatta actagagaac aaatgttaac aaacagattt aatgatcttt
     6961 tggataggat ggatataatg tttggttcta caggctctgc tgacatagaa gaatggatgg
     7021 ctggagttgc ttggctacac tgtttgttgc ccaaaatgga ttcagtggtg tatgactttt
     7081 taaaatgcat ggtgtacaac attcctaaaa aaagatactg gctgtttaaa ggaccaattg
     7141 atagtggtaa aactacatta gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     7201 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     7261 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     7321 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     7381 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     7441 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8041 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8281 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8341 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8401 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8461 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8521 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8581 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8641 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8701 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8761 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8821 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8881 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8941 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9001 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9061 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9361 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     9421 aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//
