LOCUS       V002946                 9459 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002946
VERSION     V002946
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9459)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        123..420
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3368..3865)
                     /gene="IFNG"
                     /label="Interferon gamma"
                     /note="Interferon gamma from Homo sapiens. Accession#:
                     P01579"
     misc_feature    complement(3962..4899)
                     /label="intron"
                     /note="intron"
     CDS             complement(4906..5091)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5135..5492
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    5506..6034
                     /label="SV40 small t-antigen"
                     /note="SV40 small t-antigen"
     misc_feature    5506..5751
                     /label="SV40 large T-antigen"
                     /note="SV40 large T-antigen"
     misc_feature    5752..6101
                     /label="intron"
                     /note="intron"
     intron          6036..6101
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6231..6251
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7306..7446
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7632..8220)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8394..9251)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9252..9356)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tcttgcttag gttggctgcc tagttggccc ctgagataaa
     3121 gccttgtaat cacatagcct tgcctaatta gtcagaaaac aaaggattaa gtgagacagt
     3181 cacaggatat aggaattata aataatacat atattaatag atattcattt tcattacaca
     3241 aaagttgcta ttataaatac ttatttgatt gatgagtcta aaaatatatt ccccatataa
     3301 ataatgttaa atattaataa atagatttag atttaaaatt caaatattgc aggcaggaca
     3361 accattactg ggatgctctt cgacctcgaa acagcatctg actccttttt cgcttccctg
     3421 ttttagctgc tggcgacagt tcagccatca cttggatgag ttcatgtatt gctttgcgtt
     3481 ggacattcaa gtcagttacc gaataattag tcagcttttc gaagtcatct cgtttctttt
     3541 tgttgctatt gaaaaacttg acattcatgt cttccttgat ggtctccaca ctcttttgga
     3601 tgctctggtc atctttaaag tttttaaaaa gtttgaagta aaaggagaca atttggctct
     3661 gcattatttt tctgtcactc tcctctttcc aattcttcaa aatgcctaag aaaagagttc
     3721 cattatccgc tacatctgaa tgacctgcat taaaatattt cttaaggttt tctgcttctt
     3781 ttacatatgg gtcctggcag taacagccaa gagaacccaa aacgatgcag agctgaaaag
     3841 ccaagatata acttgtatat ttcatcgttt ccgagagaat taagccaaag aagttgaaat
     3901 cagtagttct tgtatcaagc tgatccggag cttttagagc agaagtaaca cttccgtaca
     3961 ggcctagaag taaaggcaac atccactgag gagcagttct ttgatttgca ccaccaggag
     4021 cctcaaattt ttcaataaat tcacctgact gcacattagg actttggctt tgagcttccc
     4081 acctctcagt tacttgctga atactgtctg cttcatcaat attatcatag gtgtgcccaa
     4141 atgatatttg caacccttcc ctgttggcta cttgtctcac cattgtaggc ctaatgggag
     4201 acaaagtaga gtagtaatct tgtaaagagt tataccaatt aacaggagca ttaattactg
     4261 tccaagtagt ttcctctaaa aaccttgcca aactgtccct taaatatctt tgggttcttc
     4321 tttcaagctc ctgtgaggtg agcctaggaa tgtcattttg tattacacgc caaaaagctt
     4381 gagaaatggc attaaaaagt gttggacccc aatgtctggg gtcaagatac tgaacactgt
     4441 gaacaaaggt ttgtactcca ggaaataaaa tatcatagta atcatctggc ctatacaaat
     4501 ctacagccat tcctggttgt tgatataaac caacagtaga aactttgtga tcccagtcac
     4561 taaaaaatct ataccccact tgagcaacag cgctcacacc agtcacagtt tgcagtaaag
     4621 ctgcaaatcc agctatagca gcaggagccc cagatatcac agcataggcc tgtggagtga
     4681 ggcctatagc agcaattgcc tcagaggttg ttaggccttc aacagtagca acagatgcaa
     4741 gttgcacttc aattgcagca gcggcctctc cagcagcaat ttcagctact gaaaatccag
     4801 tagcagcagc agcttcagac acagtagcaa ttaggtcccc caacagtgtt aaagcagcac
     4861 ccatggacct gaaataaaag acaaaaagac taaacttacc agttaacttt ctggtttttc
     4921 agttaacctt tctggttttt tgcgtttccc gtcaacagta tcttcccctt cacaaaattg
     4981 cagcaaaagc tctaaaacaa acacaaaaag gcgttgagct gtttttttac tttcagtcca
     5041 tgacctacga accttaacgg aggcctggcg tgacagccgg cgcagcacca tggcctgaaa
     5101 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga
     5161 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5221 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5281 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5461 gaggcttttt tggaggccta ggcttttgca aaaagctttg caaagatgga taaagtttta
     5521 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgcctggggg
     5581 aatattcctc tgatgagaaa ggcatattta aaaaaatgca aggagtttca tcctgataaa
     5641 ggaggagatg aagaaaaaat gaagaaaatg aatactctgt acaagaaaat ggaagatgga
     5701 gtaaaatatg ctcatcaacc tgactttgga ggcttctggg atgcaactga ggtatttgct
     5761 tcttccttaa atcctggtgt tgatgcaatg tactgcaaac aatggcctga gtgtgcaaag
     5821 aaaatgtctg ctaactgcat atgcttgctg tgcttactga ggatgaagca tgaaaataga
     5881 aaattataca ggaaagatcc accagatctg gtgggttgat tgctactgct tcgattgctt
     5941 tagaatgtgg tttggacttg atctttgtga aggaacctta cttctgtggt gtgacataat
     6001 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     6061 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
     6121 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
     6181 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
     6241 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
     6301 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
     6361 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
     6421 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
     6481 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
     6541 aatatttgat gtatagtgcc ttgactagag atccattttc tgttattgag gaaagtttgc
     6601 caggtgggtt aaaggagcat gattttaatc cagaagaagc agaggaaact aaacaagtgt
     6661 cctggaagct tgtaacagag tatgcaatgg aaacaaaatg tgatgatgtg ttgttattgc
     6721 ttgggatgta cttggaattt cagtacagtt ttgaaatgtg tttaaaatgt attaaaaaag
     6781 aacagcccag ccactataag taccatgaaa agcattatgc aaatgctgct atatttgctg
     6841 acagcaaaaa ccaaaaaacc atatgccaac aggctgttga tactgtttta gctaaaaagc
     6901 gggttgatag cctacaatta actagagaac aaatgttaac aaacagattt aatgatcttt
     6961 tggataggat ggatataatg tttggttcta caggctctgc tgacatagaa gaatggatgg
     7021 ctggagttgc ttggctacac tgtttgttgc ccaaaatgga ttcagtggtg tatgactttt
     7081 taaaatgcat ggtgtacaac attcctaaaa aaagatactg gctgtttaaa ggaccaattg
     7141 atagtggtaa aactacatta gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     7201 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     7261 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     7321 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     7381 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     7441 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8041 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8281 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8341 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8401 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8461 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8521 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8581 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8641 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8701 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8761 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8821 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8881 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8941 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9001 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9061 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9361 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     9421 aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//
