LOCUS       V002947                 9455 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002947
VERSION     V002947
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9455)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9455)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3368..3865)
                     /gene="IFNG"
                     /label="Interferon gamma"
                     /note="Interferon gamma from Homo sapiens. Accession#:
                     P01579"
     misc_feature    complement(3962..4899)
                     /label="intron"
                     /note="intron"
     CDS             complement(4906..5091)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5163..5492
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5506..6027
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6227..6247
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7302..7442
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7628..8216)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8390..9247)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9248..9352)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tcttgcttag gttggctgcc tagttggccc ctgagataaa
     3121 gccttgtaat cacatagcct tgcctaatta gtcagaaaac aaaggattaa gtgagacagt
     3181 cacaggatat aggaattata aataatacat atattaatag atattcattt tcattacaca
     3241 aaagttgcta ttataaatac ttatttgatt gatgagtcta aaaatatatt ccccatataa
     3301 ataatgttaa atattaataa atagatttag atttaaaatt caaatattgc aggcaggaca
     3361 accattactg ggatgctctt cgacctcgaa acagcatctg actccttttt cgcttccctg
     3421 ttttagctgc tggcgacagt tcagccatca cttggatgag ttcatgtatt gctttgcgtt
     3481 ggacattcaa gtcagttacc gaataattag tcagcttttc gaagtcatct cgtttctttt
     3541 tgttgctatt gaaaaacttg acattcatgt cttccttgat ggtctccaca ctcttttgga
     3601 tgctctggtc atctttaaag tttttaaaaa gtttgaagta aaaggagaca atttggctct
     3661 gcattatttt tctgtcactc tcctctttcc aattcttcaa aatgcctaag aaaagagttc
     3721 cattatccgc tacatctgaa tgacctgcat taaaatattt cttaaggttt tctgcttctt
     3781 ttacatatgg gtcctggcag taacagccaa gagaacccaa aacgatgcag agctgaaaag
     3841 ccaagatata acttgtatat ttcatcgttt ccgagagaat taagccaaag aagttgaaat
     3901 cagtagttct tgtatcaagc tgatccggag cttttagagc agaagtaaca cttccgtaca
     3961 ggcctagaag taaaggcaac atccactgag gagcagttct ttgatttgca ccaccaggag
     4021 cctcaaattt ttcaataaat tcacctgact gcacattagg actttggctt tgagcttccc
     4081 acctctcagt tacttgctga atactgtctg cttcatcaat attatcatag gtgtgcccaa
     4141 atgatatttg caacccttcc ctgttggcta cttgtctcac cattgtaggc ctaatgggag
     4201 acaaagtaga gtagtaatct tgtaaagagt tataccaatt aacaggagca ttaattactg
     4261 tccaagtagt ttcctctaaa aaccttgcca aactgtccct taaatatctt tgggttcttc
     4321 tttcaagctc ctgtgaggtg agcctaggaa tgtcattttg tattacacgc caaaaagctt
     4381 gagaaatggc attaaaaagt gttggacccc aatgtctggg gtcaagatac tgaacactgt
     4441 gaacaaaggt ttgtactcca ggaaataaaa tatcatagta atcatctggc ctatacaaat
     4501 ctacagccat tcctggttgt tgatataaac caacagtaga aactttgtga tcccagtcac
     4561 taaaaaatct ataccccact tgagcaacag cgctcacacc agtcacagtt tgcagtaaag
     4621 ctgcaaatcc agctatagca gcaggagccc cagatatcac agcataggcc tgtggagtga
     4681 ggcctatagc agcaattgcc tcagaggttg ttaggccttc aacagtagca acagatgcaa
     4741 gttgcacttc aattgcagca gcggcctctc cagcagcaat ttcagctact gaaaatccag
     4801 tagcagcagc agcttcagac acagtagcaa ttaggtcccc caacagtgtt aaagcagcac
     4861 ccatggacct gaaataaaag acaaaaagac taaacttacc agttaacttt ctggtttttc
     4921 agttaacctt tctggttttt tgcgtttccc gtcaacagta tcttcccctt cacaaaattg
     4981 cagcaaaagc tctaaaacaa acacaaaaag gcgttgagct gtttttttac tttcagtcca
     5041 tgacctacga accttaacgg aggcctggcg tgacagccgg cgcagcacca tggcctgaaa
     5101 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga
     5161 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5221 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5281 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5461 gaggcttttt tggaggccta ggcttttgca aaaagctttg caaagatgga taaagtttta
     5521 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgcctggggg
     5581 aatattcctc tgatgagaaa ggcatattta aaaaaatgca aggagtttca tcctgataaa
     5641 ggaggagatg aagaaaaaat gaagaaaatg aatactctgt acaagaaaat ggaagatgga
     5701 gtaaaatatg ctcatcaacc tgactttgga ggcttctggg atgcaactga ggtatttgct
     5761 tcttccttaa atcctggtgt tgatgcaatg tactgcaaac aatggcctga gtgtgcaaag
     5821 aaaatgtctg ctaactgcat atgcttgctg tgcttactga ggatgaagca tgaaaataga
     5881 aaattataca ggaaagatcc acttgtgtgg gttgattgct actgcttcga ttgctttaga
     5941 atgtggtttg gacttgatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     6001 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     6061 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     6121 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     6181 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     6241 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     6301 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     6361 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     6421 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     6481 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     6541 tttgatgtat agtgccttga ctagagatcc attttctgtt attgaggaaa gtttgccagg
     6601 tgggttaaag gagcatgatt ttaatccaga agaagcagag gaaactaaac aagtgtcctg
     6661 gaagcttgta acagagtatg caatggaaac aaaatgtgat gatgtgttgt tattgcttgg
     6721 gatgtacttg gaatttcagt acagttttga aatgtgttta aaatgtatta aaaaagaaca
     6781 gcccagccac tataagtacc atgaaaagca ttatgcaaat gctgctatat ttgctgacag
     6841 caaaaaccaa aaaaccatat gccaacaggc tgttgatact gttttagcta aaaagcgggt
     6901 tgatagccta caattaacta gagaacaaat gttaacaaac agatttaatg atcttttgga
     6961 taggatggat ataatgtttg gttctacagg ctctgctgac atagaagaat ggatggctgg
     7021 agttgcttgg ctacactgtt tgttgcccaa aatggattca gtggtgtatg actttttaaa
     7081 atgcatggtg tacaacattc ctaaaaaaag atactggctg tttaaaggac caattgatag
     7141 tggtaaaact acattagcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     7201 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     7261 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     7321 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     7381 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7441 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7501 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7561 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7801 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8041 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8221 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8281 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8341 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8401 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8461 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8521 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8581 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8641 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8701 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8761 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8821 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8881 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8941 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9001 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9061 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9121 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9181 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9241 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9301 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9361 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9421 ataggcgtat cacgaggccc tttcgtcttc aagaa
//
