LOCUS       V002948                 8612 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002948
VERSION     V002948
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8612)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3119..4056)
                     /label="intron"
                     /note="intron"
     CDS             complement(4063..4248)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4320..4649
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4663..5184
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             5384..5404
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    6459..6599
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6785..7373)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7547..8404)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8405..8509)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggagctt ttagagcaga agtaacactt ccgtacaggc
     3121 ctagaagtaa aggcaacatc cactgaggag cagttctttg atttgcacca ccaggagcct
     3181 caaatttttc aataaattca cctgactgca cattaggact ttggctttga gcttcccacc
     3241 tctcagttac ttgctgaata ctgtctgctt catcaatatt atcataggtg tgcccaaatg
     3301 atatttgcaa cccttccctg ttggctactt gtctcaccat tgtaggccta atgggagaca
     3361 aagtagagta gtaatcttgt aaagagttat accaattaac aggagcatta attactgtcc
     3421 aagtagtttc ctctaaaaac cttgccaaac tgtcccttaa atatctttgg gttcttcttt
     3481 caagctcctg tgaggtgagc ctaggaatgt cattttgtat tacacgccaa aaagcttgag
     3541 aaatggcatt aaaaagtgtt ggaccccaat gtctggggtc aagatactga acactgtgaa
     3601 caaaggtttg tactccagga aataaaatat catagtaatc atctggccta tacaaatcta
     3661 cagccattcc tggttgttga tataaaccaa cagtagaaac tttgtgatcc cagtcactaa
     3721 aaaatctata ccccacttga gcaacagcgc tcacaccagt cacagtttgc agtaaagctg
     3781 caaatccagc tatagcagca ggagccccag atatcacagc ataggcctgt ggagtgaggc
     3841 ctatagcagc aattgcctca gaggttgtta ggccttcaac agtagcaaca gatgcaagtt
     3901 gcacttcaat tgcagcagcg gcctctccag cagcaatttc agctactgaa aatccagtag
     3961 cagcagcagc ttcagacaca gtagcaatta ggtcccccaa cagtgttaaa gcagcaccca
     4021 tggacctgaa ataaaagaca aaaagactaa acttaccagt taactttctg gtttttcagt
     4081 taacctttct ggttttttgc gtttcccgtc aacagtatct tccccttcac aaaattgcag
     4141 caaaagctct aaaacaaaca caaaaaggcg ttgagctgtt tttttacttt cagtccatga
     4201 cctacgaacc ttaacggagg cctggcgtga cagccggcgc agcaccatgg cctgaaataa
     4261 cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg
     4321 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4381 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4441 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4501 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4561 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4621 gcttttttgg aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac
     4681 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat
     4741 attcctctga tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga
     4801 ggagatgaag aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta
     4861 aaatatgctc atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct
     4921 tccttaaatc ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa
     4981 atgtctgcta actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa
     5041 ttatacagga aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg
     5101 tggtttggac ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     5161 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     5221 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     5281 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     5341 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     5401 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     5461 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     5521 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     5581 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     5641 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     5701 gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg
     5761 gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa
     5821 gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat
     5881 gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc
     5941 cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa
     6001 aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga
     6061 tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag
     6121 gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt
     6181 tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg
     6241 catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg
     6301 taaaactaca ttagcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     6361 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     6421 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     6481 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     6541 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6601 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6661 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6721 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6781 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6841 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6901 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6961 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7021 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7081 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7141 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7201 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     7261 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7321 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7381 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7441 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7501 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7561 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7621 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7681 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7741 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7801 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     7861 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7921 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7981 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8041 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8101 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8161 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8221 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8281 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8341 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8401 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8461 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8521 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     8581 ggcgtatcac gaggcccttt cgtcttcaag aa
//
