LOCUS       V002953                10284 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002953
VERSION     V002953
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10284)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10284)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3104..3194)
                     /label="3' UTR of mIghg2b"
                     /note="3' UTR of mIghg2b"
     CDS             complement(3198..4205)
                     /label="mlgG2b-CH"
                     /note="Mouse IgG2b heavy chain constant region"
     misc_feature    complement(4791..5728)
                     /label="intron"
                     /note="intron"
     CDS             complement(5735..5920)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5992..6321
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6335..6856
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             7056..7076
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    8131..8271
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8457..9045)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9219..10076)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10077..10181)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagtcgactc tagcaggctt tgctggatgc
     3121 tttatttata caagggatgc atggatatgg gtgccagtgt ctcttaggac ctgagagctt
     3181 tgtgggtgct gagctcattt acccggagac cgggagatgg tcttcttcag gtagtaattt
     3241 ttcagaccct cgtgtctcac gttgcatgag aaggaatctg ttttctccca cttgcttgtt
     3301 ttcatattga gcttgctata tatgaagtaa gaaccgtcag agtctaggac tgggcctttg
     3361 tccttgtagt tctcctctgt atgcccattg ctggtccact ccacactgat gtcttcaggg
     3421 ctgaagccca cggccaggca agtcagactg acatctttcc tggacaactg ctctggtggt
     3481 ggcgacaaga tgtatacttg tggagctctg actatccctt taatttttga gatggttctc
     3541 tcaatgggcg ctgggaggtc tttgttgttg accttgcatt tgaactcctt gccactcatc
     3601 cagtcctggt gctggatggg gagggcactg accacccgga tagtactgtt gtaatcctct
     3661 ctatgggttt gtgtctgagc tgtgagtact tccacgttgt tcacaaacca gctgatctgg
     3721 acgtctgggt catcctcgct cacatccacc accacacacg tgaccttggg tgtcagggag
     3781 atcatgagta catccttgat atttggaggg aagatgaaga cggatggtcc accctcgagg
     3841 ttaggagctg ggcatttgtg acactccttg catggaggac aggggttgat tgttgaagtg
     3901 ggcccgctgg gctcaagttt tttgtccacc gtggtgctgc tggctgggtg agcaacactg
     3961 caggtaacgg tctggcttgg ccaggtgctg gaggggacag tcactgagct gctcatagtg
     4021 tagagtccag actgcaggag agctgggaag gtgtgcacac tgctggacag ggatccggag
     4081 ttccaagtca cagtcactga ctcagggaag tagcccttga ccaggcatcc cagagtcacg
     4141 gaggaaccag ttgtatctcc acacccaggg gccagtggat agactgatgg gggtgttgtt
     4201 ttggctgcag aggcagtgac cagagtccct tggccccagt aaccaaaagg gagtagcccg
     4261 acctgtctag gtccggcaca gaaatagacg gcagagtcct cagatgtcag gctgctgagg
     4321 tgcaagtagg ctgtgctgga ggatttgtct acagtcagtg tggccttgcc cttaaacttc
     4381 tcattgaagt acgaatttcc acttccagga taaatttctc caatccactc aaggccctgt
     4441 ccagttctct gcttcaccca acttatacca taacttgtga gggtgtatcc agaggccttg
     4501 caggacagct tcactgaggc cccaggcctc gccagctcag ctccagactg ctgcagctga
     4561 acctgggatt ggacacctgc agttcctgac aggatgaaga gaaagatcca gatccattcc
     4621 atgctagagc caagcttggc cggatccgga attccaatgc ttcgtttcgt atcacacacc
     4681 ccaaagcctt ctgctttgaa tgctgccctt cttcagggct taatttctct agaggctgca
     4741 gccgaccccg gatccggagc ttttagagca gaagtaacac ttccgtacag gcctagaagt
     4801 aaaggcaaca tccactgagg agcagttctt tgatttgcac caccaggagc ctcaaatttt
     4861 tcaataaatt cacctgactg cacattagga ctttggcttt gagcttccca cctctcagtt
     4921 acttgctgaa tactgtctgc ttcatcaata ttatcatagg tgtgcccaaa tgatatttgc
     4981 aacccttccc tgttggctac ttgtctcacc attgtaggcc taatgggaga caaagtagag
     5041 tagtaatctt gtaaagagtt ataccaatta acaggagcat taattactgt ccaagtagtt
     5101 tcctctaaaa accttgccaa actgtccctt aaatatcttt gggttcttct ttcaagctcc
     5161 tgtgaggtga gcctaggaat gtcattttgt attacacgcc aaaaagcttg agaaatggca
     5221 ttaaaaagtg ttggacccca atgtctgggg tcaagatact gaacactgtg aacaaaggtt
     5281 tgtactccag gaaataaaat atcatagtaa tcatctggcc tatacaaatc tacagccatt
     5341 cctggttgtt gatataaacc aacagtagaa actttgtgat cccagtcact aaaaaatcta
     5401 taccccactt gagcaacagc gctcacacca gtcacagttt gcagtaaagc tgcaaatcca
     5461 gctatagcag caggagcccc agatatcaca gcataggcct gtggagtgag gcctatagca
     5521 gcaattgcct cagaggttgt taggccttca acagtagcaa cagatgcaag ttgcacttca
     5581 attgcagcag cggcctctcc agcagcaatt tcagctactg aaaatccagt agcagcagca
     5641 gcttcagaca cagtagcaat taggtccccc aacagtgtta aagcagcacc catggacctg
     5701 aaataaaaga caaaaagact aaacttacca gttaactttc tggtttttca gttaaccttt
     5761 ctggtttttt gcgtttcccg tcaacagtat cttccccttc acaaaattgc agcaaaagct
     5821 ctaaaacaaa cacaaaaagg cgttgagctg tttttttact ttcagtccat gacctacgaa
     5881 ccttaacgga ggcctggcgt gacagccggc gcagcaccat ggcctgaaat aacctctgaa
     5941 agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     6001 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6061 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6121 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6181 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6241 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6301 ggaggcctag gcttttgcaa aaagctttgc aaagatggat aaagttttaa acagagagga
     6361 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gcctggggga atattcctct
     6421 gatgagaaag gcatatttaa aaaaatgcaa ggagtttcat cctgataaag gaggagatga
     6481 agaaaaaatg aagaaaatga atactctgta caagaaaatg gaagatggag taaaatatgc
     6541 tcatcaacct gactttggag gcttctggga tgcaactgag gtatttgctt cttccttaaa
     6601 tcctggtgtt gatgcaatgt actgcaaaca atggcctgag tgtgcaaaga aaatgtctgc
     6661 taactgcata tgcttgctgt gcttactgag gatgaagcat gaaaatagaa aattatacag
     6721 gaaagatcca cttgtgtggg ttgattgcta ctgcttcgat tgctttagaa tgtggtttgg
     6781 acttgatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     6841 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     6901 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     6961 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     7021 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     7081 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     7141 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     7201 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     7261 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     7321 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     7381 gtgccttgac tagagatcca ttttctgtta ttgaggaaag tttgccaggt gggttaaagg
     7441 agcatgattt taatccagaa gaagcagagg aaactaaaca agtgtcctgg aagcttgtaa
     7501 cagagtatgc aatggaaaca aaatgtgatg atgtgttgtt attgcttggg atgtacttgg
     7561 aatttcagta cagttttgaa atgtgtttaa aatgtattaa aaaagaacag cccagccact
     7621 ataagtacca tgaaaagcat tatgcaaatg ctgctatatt tgctgacagc aaaaaccaaa
     7681 aaaccatatg ccaacaggct gttgatactg ttttagctaa aaagcgggtt gatagcctac
     7741 aattaactag agaacaaatg ttaacaaaca gatttaatga tcttttggat aggatggata
     7801 taatgtttgg ttctacaggc tctgctgaca tagaagaatg gatggctgga gttgcttggc
     7861 tacactgttt gttgcccaaa atggattcag tggtgtatga ctttttaaaa tgcatggtgt
     7921 acaacattcc taaaaaaaga tactggctgt ttaaaggacc aattgatagt ggtaaaacta
     7981 cattagcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     8041 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     8101 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     8161 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     8221 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     8281 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8341 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8401 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8461 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8521 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8581 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8641 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8701 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8761 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8821 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8881 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8941 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9001 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9061 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9121 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9181 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9241 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9301 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9361 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9421 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9481 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     9541 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9601 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9661 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9721 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9781 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     9841 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9901 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9961 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10021 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10081 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10141 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10201 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10261 acgaggccct ttcgtcttca agaa
//
