LOCUS       V002956                 9574 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002956
VERSION     V002956
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9574)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3112..3912)
                     /codon_start=1
                     /note="unnamed protein product; mIghg2b; fragment of heavy
                     chain of mouse anti-hPLAP monoclonal antibody (E6H)"
                     /protein_id="SJL89005.1"
                     /translation="MEWIWIFLFILSGTAGVQSQVQLQQSGAELARPGASVKLSCKASG
                     YTLTSYGISWVKQRTGQGLEWIGEIYPGSGNSYFNEKFKGKATLTVDKSSSTAYLHLSS
                     LTSEDSAVYFCAGPRQVGLLPFGYWGQGTLVTASAAKTTPPSVYPLAPGCGDTTGSSVT
                     LGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCS
                     VAHPASSTTVDKKLEPSGPTSTINPCPPCKECHKCPAPNLDLVN"
     misc_feature    complement(4081..5018)
                     /label="intron"
                     /note="intron"
     CDS             complement(5025..5210)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5282..5611
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5625..6146
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6346..6366
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7421..7561
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7747..8335)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8509..9366)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9367..9471)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagtcgacct gcagcccaag cttagttaac
     3121 taagtcgagg ttaggagctg ggcatttgtg acactccttg catggaggac aggggttgat
     3181 tgttgaagtg ggcccgctgg gctcaagttt tttgtccacc gtggtgctgc tggctgggtg
     3241 agcaacactg caggtaacgg tctggcttgg ccaggtgctg gaggggacag tcactgagct
     3301 gctcatagtg tagagtccag actgcaggag agctgggaag gtgtgcacac tgctggacag
     3361 ggatccggag ttccaagtca cagtcactga ctcagggaag tagcccttga ccaggcatcc
     3421 cagagtcacg gaggaaccag ttgtatctcc acacccaggg gccagtggat agactgatgg
     3481 gggtgttgtt ttggctgcag aggcagtgac cagagtccct tggccccagt aaccaaaagg
     3541 gagtagcccg acctgtctag gtccggcaca gaaatagacg gcagagtcct cagatgtcag
     3601 gctgctgagg tgcaagtagg ctgtgctgga ggatttgtct acagtcagtg tggccttgcc
     3661 cttaaacttc tcattgaagt acgaatttcc acttccagga taaatttctc caatccactc
     3721 aaggccctgt ccagttctct gcttcaccca acttatacca taacttgtga gggtgtatcc
     3781 agaggccttg caggacagct tcactgaggc cccaggcctc gccagctcag ctccagactg
     3841 ctgcagctga acctgggatt ggacacctgc agttcctgac aggatgaaga gaaagatcca
     3901 gatccattcc atgctagagc caagcttggc cggatccgga attccaatgc ttcgtttcgt
     3961 atcacacacc ccaaagcctt ctgctttgaa tgctgccctt cttcagggct taatttctct
     4021 agaggctgca gccgaccccg gatccggagc ttttagagca gaagtaacac ttccgtacag
     4081 gcctagaagt aaaggcaaca tccactgagg agcagttctt tgatttgcac caccaggagc
     4141 ctcaaatttt tcaataaatt cacctgactg cacattagga ctttggcttt gagcttccca
     4201 cctctcagtt acttgctgaa tactgtctgc ttcatcaata ttatcatagg tgtgcccaaa
     4261 tgatatttgc aacccttccc tgttggctac ttgtctcacc attgtaggcc taatgggaga
     4321 caaagtagag tagtaatctt gtaaagagtt ataccaatta acaggagcat taattactgt
     4381 ccaagtagtt tcctctaaaa accttgccaa actgtccctt aaatatcttt gggttcttct
     4441 ttcaagctcc tgtgaggtga gcctaggaat gtcattttgt attacacgcc aaaaagcttg
     4501 agaaatggca ttaaaaagtg ttggacccca atgtctgggg tcaagatact gaacactgtg
     4561 aacaaaggtt tgtactccag gaaataaaat atcatagtaa tcatctggcc tatacaaatc
     4621 tacagccatt cctggttgtt gatataaacc aacagtagaa actttgtgat cccagtcact
     4681 aaaaaatcta taccccactt gagcaacagc gctcacacca gtcacagttt gcagtaaagc
     4741 tgcaaatcca gctatagcag caggagcccc agatatcaca gcataggcct gtggagtgag
     4801 gcctatagca gcaattgcct cagaggttgt taggccttca acagtagcaa cagatgcaag
     4861 ttgcacttca attgcagcag cggcctctcc agcagcaatt tcagctactg aaaatccagt
     4921 agcagcagca gcttcagaca cagtagcaat taggtccccc aacagtgtta aagcagcacc
     4981 catggacctg aaataaaaga caaaaagact aaacttacca gttaactttc tggtttttca
     5041 gttaaccttt ctggtttttt gcgtttcccg tcaacagtat cttccccttc acaaaattgc
     5101 agcaaaagct ctaaaacaaa cacaaaaagg cgttgagctg tttttttact ttcagtccat
     5161 gacctacgaa ccttaacgga ggcctggcgt gacagccggc gcagcaccat ggcctgaaat
     5221 aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa
     5281 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5341 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     5401 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5461 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5521 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5581 aggctttttt ggaggcctag gcttttgcaa aaagctttgc aaagatggat aaagttttaa
     5641 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gcctggggga
     5701 atattcctct gatgagaaag gcatatttaa aaaaatgcaa ggagtttcat cctgataaag
     5761 gaggagatga agaaaaaatg aagaaaatga atactctgta caagaaaatg gaagatggag
     5821 taaaatatgc tcatcaacct gactttggag gcttctggga tgcaactgag gtatttgctt
     5881 cttccttaaa tcctggtgtt gatgcaatgt actgcaaaca atggcctgag tgtgcaaaga
     5941 aaatgtctgc taactgcata tgcttgctgt gcttactgag gatgaagcat gaaaatagaa
     6001 aattatacag gaaagatcca cttgtgtggg ttgattgcta ctgcttcgat tgctttagaa
     6061 tgtggtttgg acttgatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     6121 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     6181 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     6241 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     6301 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     6361 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     6421 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     6481 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     6541 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     6601 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     6661 ttgatgtata gtgccttgac tagagatcca ttttctgtta ttgaggaaag tttgccaggt
     6721 gggttaaagg agcatgattt taatccagaa gaagcagagg aaactaaaca agtgtcctgg
     6781 aagcttgtaa cagagtatgc aatggaaaca aaatgtgatg atgtgttgtt attgcttggg
     6841 atgtacttgg aatttcagta cagttttgaa atgtgtttaa aatgtattaa aaaagaacag
     6901 cccagccact ataagtacca tgaaaagcat tatgcaaatg ctgctatatt tgctgacagc
     6961 aaaaaccaaa aaaccatatg ccaacaggct gttgatactg ttttagctaa aaagcgggtt
     7021 gatagcctac aattaactag agaacaaatg ttaacaaaca gatttaatga tcttttggat
     7081 aggatggata taatgtttgg ttctacaggc tctgctgaca tagaagaatg gatggctgga
     7141 gttgcttggc tacactgttt gttgcccaaa atggattcag tggtgtatga ctttttaaaa
     7201 tgcatggtgt acaacattcc taaaaaaaga tactggctgt ttaaaggacc aattgatagt
     7261 ggtaaaacta cattagcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     7321 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7381 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     7441 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     7501 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     7561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8161 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8641 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8701 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8761 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8821 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8881 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8941 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9001 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9061 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9121 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9181 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9241 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9301 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9361 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9421 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9481 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     9541 taggcgtatc acgaggccct ttcgtcttca agaa
//
