LOCUS       V002957                 9472 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002957
VERSION     V002957
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9472)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9472)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3088..3810)
                     /codon_start=1
                     /note="unnamed protein product; mIghg2b; fragment of heavy
                     chain of mouse anti-hPLAP monoclonal antibody (E6H)"
                     /protein_id="SJL89001.1"
                     /translation="MEWIWIFLFILSGTAGVQSQVQLQQSGAELARPGASVKLSCKASG
                     YTLTSYGISWVKQRTGQGLEWIGEIYPGSGNSYFNEKFKGKATLTVDKSSSTAYLHLSS
                     LTSEDSAVYFCAGPRQVGLLPFGYWGQGTLVTASAAKTTPPSVYPLAPGCGDTTGSSVT
                     LGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCS
                     VAHPASSTTVDKKLEPSV"
     misc_feature    complement(3979..4916)
                     /label="intron"
                     /note="intron"
     CDS             complement(4923..5108)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5180..5509
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5523..6044
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6244..6264
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7319..7459
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7645..8233)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8407..9264)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9265..9369)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gacgctgggc tcaagttttt tgtccaccgt
     3121 ggtgctgctg gctgggtgag caacactgca ggtaacggtc tggcttggcc aggtgctgga
     3181 ggggacagtc actgagctgc tcatagtgta gagtccagac tgcaggagag ctgggaaggt
     3241 gtgcacactg ctggacaggg atccggagtt ccaagtcaca gtcactgact cagggaagta
     3301 gcccttgacc aggcatccca gagtcacgga ggaaccagtt gtatctccac acccaggggc
     3361 cagtggatag actgatgggg gtgttgtttt ggctgcagag gcagtgacca gagtcccttg
     3421 gccccagtaa ccaaaaggga gtagcccgac ctgtctaggt ccggcacaga aatagacggc
     3481 agagtcctca gatgtcaggc tgctgaggtg caagtaggct gtgctggagg atttgtctac
     3541 agtcagtgtg gccttgccct taaacttctc attgaagtac gaatttccac ttccaggata
     3601 aatttctcca atccactcaa ggccctgtcc agttctctgc ttcacccaac ttataccata
     3661 acttgtgagg gtgtatccag aggccttgca ggacagcttc actgaggccc caggcctcgc
     3721 cagctcagct ccagactgct gcagctgaac ctgggattgg acacctgcag ttcctgacag
     3781 gatgaagaga aagatccaga tccattccat gctagagcca agcttggccg gatccggaat
     3841 tccaatgctt cgtttcgtat cacacacccc aaagccttct gctttgaatg ctgcccttct
     3901 tcagggctta atttctctag aggctgcagc cgaccccgga tccggagctt ttagagcaga
     3961 agtaacactt ccgtacaggc ctagaagtaa aggcaacatc cactgaggag cagttctttg
     4021 atttgcacca ccaggagcct caaatttttc aataaattca cctgactgca cattaggact
     4081 ttggctttga gcttcccacc tctcagttac ttgctgaata ctgtctgctt catcaatatt
     4141 atcataggtg tgcccaaatg atatttgcaa cccttccctg ttggctactt gtctcaccat
     4201 tgtaggccta atgggagaca aagtagagta gtaatcttgt aaagagttat accaattaac
     4261 aggagcatta attactgtcc aagtagtttc ctctaaaaac cttgccaaac tgtcccttaa
     4321 atatctttgg gttcttcttt caagctcctg tgaggtgagc ctaggaatgt cattttgtat
     4381 tacacgccaa aaagcttgag aaatggcatt aaaaagtgtt ggaccccaat gtctggggtc
     4441 aagatactga acactgtgaa caaaggtttg tactccagga aataaaatat catagtaatc
     4501 atctggccta tacaaatcta cagccattcc tggttgttga tataaaccaa cagtagaaac
     4561 tttgtgatcc cagtcactaa aaaatctata ccccacttga gcaacagcgc tcacaccagt
     4621 cacagtttgc agtaaagctg caaatccagc tatagcagca ggagccccag atatcacagc
     4681 ataggcctgt ggagtgaggc ctatagcagc aattgcctca gaggttgtta ggccttcaac
     4741 agtagcaaca gatgcaagtt gcacttcaat tgcagcagcg gcctctccag cagcaatttc
     4801 agctactgaa aatccagtag cagcagcagc ttcagacaca gtagcaatta ggtcccccaa
     4861 cagtgttaaa gcagcaccca tggacctgaa ataaaagaca aaaagactaa acttaccagt
     4921 taactttctg gtttttcagt taacctttct ggttttttgc gtttcccgtc aacagtatct
     4981 tccccttcac aaaattgcag caaaagctct aaaacaaaca caaaaaggcg ttgagctgtt
     5041 tttttacttt cagtccatga cctacgaacc ttaacggagg cctggcgtga cagccggcgc
     5101 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     5161 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5341 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5461 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttgcaa
     5521 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     5581 aaaggagtgc ctgggggaat attcctctga tgagaaaggc atatttaaaa aaatgcaagg
     5641 agtttcatcc tgataaagga ggagatgaag aaaaaatgaa gaaaatgaat actctgtaca
     5701 agaaaatgga agatggagta aaatatgctc atcaacctga ctttggaggc ttctgggatg
     5761 caactgaggt atttgcttct tccttaaatc ctggtgttga tgcaatgtac tgcaaacaat
     5821 ggcctgagtg tgcaaagaaa atgtctgcta actgcatatg cttgctgtgc ttactgagga
     5881 tgaagcatga aaatagaaaa ttatacagga aagatccact tgtgtgggtt gattgctact
     5941 gcttcgattg ctttagaatg tggtttggac ttgatctttg tgaaggaacc ttacttctgt
     6001 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     6061 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     6121 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     6181 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     6241 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     6301 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     6361 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     6421 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     6481 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     6541 ggggttaata aggaatattt gatgtatagt gccttgacta gagatccatt ttctgttatt
     6601 gaggaaagtt tgccaggtgg gttaaaggag catgatttta atccagaaga agcagaggaa
     6661 actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat
     6721 gtgttgttat tgcttgggat gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa
     6781 tgtattaaaa aagaacagcc cagccactat aagtaccatg aaaagcatta tgcaaatgct
     6841 gctatatttg ctgacagcaa aaaccaaaaa accatatgcc aacaggctgt tgatactgtt
     6901 ttagctaaaa agcgggttga tagcctacaa ttaactagag aacaaatgtt aacaaacaga
     6961 tttaatgatc ttttggatag gatggatata atgtttggtt ctacaggctc tgctgacata
     7021 gaagaatgga tggctggagt tgcttggcta cactgtttgt tgcccaaaat ggattcagtg
     7081 gtgtatgact ttttaaaatg catggtgtac aacattccta aaaaaagata ctggctgttt
     7141 aaaggaccaa ttgatagtgg taaaactaca ttagcagctg cctcgcgcgt ttcggtgatg
     7201 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     7261 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     7321 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     7381 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     7441 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8281 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8341 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8401 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8461 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8521 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8581 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8641 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8701 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8761 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8821 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8881 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8941 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9001 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     9061 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9121 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9181 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9241 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9301 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9361 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     9421 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aa
//
