LOCUS       V002959                 9700 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002959
VERSION     V002959
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9700)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     terminator      complement(3097..3191)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3214..3231)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     misc_feature    complement(3961..4017)
                     /label="sB1H"
                     /note="sB1H"
     misc_feature    complement(4207..5144)
                     /label="intron"
                     /note="intron"
     CDS             complement(5151..5336)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5408..5737
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5751..6272
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6472..6492
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7547..7687
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7873..8461)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8635..9492)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9493..9597)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gcttggattc tcaccaataa aaaacgcccg
     3121 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     3181 caacaggagt ccaagctcag ctaattaagc ttagtgatgg tgatggtgat gagatccttt
     3241 cagctccagc ttggtcccag caccgaacgt gagaggatag ttgctatatt gttggcagaa
     3301 ataatctgcc aggtcttcag actgcacatt gctgatggtg agagtgaaat ctgtcccaga
     3361 tccactgcct gtgaagcgat cagggactcc agtgtaccga ctggatgtcg agtcaatcag
     3421 tagtttagga gattgtccta gtttctgttg ataccaggct acatcagtac ccacattctg
     3481 actggccttg caggtgatgc tgaccctgtc tcctactgat gtggacatga atttttgaga
     3541 ctgggtcatc acaatgtccg atccgccacc gccagagcca cctccgcctg aaccgcctcc
     3601 acctgaggag acagtgaccg tggtccctgc gccccagaca tcgaagtacc ccccccccca
     3661 gttaggactt gcacagtaat acaaggccgt gtcctcagac ctcagactgc ccatttgcag
     3721 gtacagggcg ttcctggcat tgtctctgga gatggtgaat cggcccttca cactgtctgg
     3781 atagtaggtg taattaccac cacgactaat ggttgcgacc cactccagcc tcttctccgg
     3841 agtctggcga acccaagaca tgtcataggt actgaaagcg aatccagagg ctgcacagga
     3901 gagtttcagg gaccctccag gcttcactaa gcctccccca gactccacca gcttcacttc
     3961 acactggaca ccttttaaaa ttaggacaag gaaaatcaag ctcagcccaa agttcatggt
     4021 gaggggtccg tgttcagtgc tgatcactga atgtgaggaa ctgggaattc caatgcttcg
     4081 tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc agggcttaat
     4141 ttctctagag gctgcagccg accccggatc cggagctttt agagcagaag taacacttcc
     4201 gtacaggcct agaagtaaag gcaacatcca ctgaggagca gttctttgat ttgcaccacc
     4261 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc
     4321 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg
     4381 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat
     4441 gggagacaaa gtagagtagt aatcttgtaa agagttatac caattaacag gagcattaat
     4501 tactgtccaa gtagtttcct ctaaaaacct tgccaaactg tcccttaaat atctttgggt
     4561 tcttctttca agctcctgtg aggtgagcct aggaatgtca ttttgtatta cacgccaaaa
     4621 agcttgagaa atggcattaa aaagtgttgg accccaatgt ctggggtcaa gatactgaac
     4681 actgtgaaca aaggtttgta ctccaggaaa taaaatatca tagtaatcat ctggcctata
     4741 caaatctaca gccattcctg gttgttgata taaaccaaca gtagaaactt tgtgatccca
     4801 gtcactaaaa aatctatacc ccacttgagc aacagcgctc acaccagtca cagtttgcag
     4861 taaagctgca aatccagcta tagcagcagg agccccagat atcacagcat aggcctgtgg
     4921 agtgaggcct atagcagcaa ttgcctcaga ggttgttagg ccttcaacag tagcaacaga
     4981 tgcaagttgc acttcaattg cagcagcggc ctctccagca gcaatttcag ctactgaaaa
     5041 tccagtagca gcagcagctt cagacacagt agcaattagg tcccccaaca gtgttaaagc
     5101 agcacccatg gacctgaaat aaaagacaaa aagactaaac ttaccagtta actttctggt
     5161 ttttcagtta acctttctgg ttttttgcgt ttcccgtcaa cagtatcttc cccttcacaa
     5221 aattgcagca aaagctctaa aacaaacaca aaaaggcgtt gagctgtttt tttactttca
     5281 gtccatgacc tacgaacctt aacggaggcc tggcgtgaca gccggcgcag caccatggcc
     5341 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     5401 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5461 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5701 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttgcaaag atggataaag
     5761 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct
     5821 gggggaatat tcctctgatg agaaaggcat atttaaaaaa atgcaaggag tttcatcctg
     5881 ataaaggagg agatgaagaa aaaatgaaga aaatgaatac tctgtacaag aaaatggaag
     5941 atggagtaaa atatgctcat caacctgact ttggaggctt ctgggatgca actgaggtat
     6001 ttgcttcttc cttaaatcct ggtgttgatg caatgtactg caaacaatgg cctgagtgtg
     6061 caaagaaaat gtctgctaac tgcatatgct tgctgtgctt actgaggatg aagcatgaaa
     6121 atagaaaatt atacaggaaa gatccacttg tgtgggttga ttgctactgc ttcgattgct
     6181 ttagaatgtg gtttggactt gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     6241 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     6301 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     6361 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     6421 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     6481 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     6541 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     6601 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     6661 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     6721 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     6781 gaatatttga tgtatagtgc cttgactaga gatccatttt ctgttattga ggaaagtttg
     6841 ccaggtgggt taaaggagca tgattttaat ccagaagaag cagaggaaac taaacaagtg
     6901 tcctggaagc ttgtaacaga gtatgcaatg gaaacaaaat gtgatgatgt gttgttattg
     6961 cttgggatgt acttggaatt tcagtacagt tttgaaatgt gtttaaaatg tattaaaaaa
     7021 gaacagccca gccactataa gtaccatgaa aagcattatg caaatgctgc tatatttgct
     7081 gacagcaaaa accaaaaaac catatgccaa caggctgttg atactgtttt agctaaaaag
     7141 cgggttgata gcctacaatt aactagagaa caaatgttaa caaacagatt taatgatctt
     7201 ttggatagga tggatataat gtttggttct acaggctctg ctgacataga agaatggatg
     7261 gctggagttg cttggctaca ctgtttgttg cccaaaatgg attcagtggt gtatgacttt
     7321 ttaaaatgca tggtgtacaa cattcctaaa aaaagatact ggctgtttaa aggaccaatt
     7381 gatagtggta aaactacatt agcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     7441 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7501 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     7561 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     7621 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7681 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7741 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7801 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8281 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8521 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8581 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8641 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8701 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8761 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8821 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8881 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8941 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9001 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9061 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9121 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9181 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9241 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9301 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9361 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9421 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9481 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9541 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9601 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9661 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//
