LOCUS       V002960                 9192 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002960
VERSION     V002960
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9192)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9192)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      complement(3218..3299)
                     /label="fd terminator"
                     /note="fd terminator"
                     /regulatory_class="terminator"
     terminator      3248..3296
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(3538..3586)
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     misc_feature    complement(3699..4636)
                     /label="major intron of 16S mRNA of SV40 VP1"
                     /note="major intron of 16S mRNA of SV40 VP1"
     CDS             complement(4643..4828)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4900..5229
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5243..5764
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          5768..5834
                     /note="intron of SV40 small t-antigen"
     CDS             5964..5984
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7039..7179
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7365..7953)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8127..8984)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8985..9089)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagggtcgac cctctagagg tcgaaattct
     3121 gtgtgaaatt gttatccgct cagaattttc tgtatgaggt tttgctaaac aactttcaac
     3181 agtttcagcg gagtgagaat agaaaggaac aactaaagga attgcgaata ataatttttt
     3241 cacgttgaaa atctccaaaa aaaaaggctc caaaaggagc ctttaattgt atcggtttat
     3301 cagcttgctt tcgaggtgaa tttcgaccct ctagagggtc gaccctctag aggctgcagc
     3361 cgaccccgga tccggctcta gagggtcgac cctctagagg tcgaaattct gtgtgaaatt
     3421 gttatccgct cagaattttc tgtatgaggt tttgctaaac aactttcaac agtttcagcg
     3481 gagtgagaat agaaaggaac aactaaagga attgcgaata ataatttttt cacgttgaaa
     3541 atctccaaaa aaaaaggctc caaaaggagc ctttaattgt atcggtttat cagcttgctt
     3601 tcgaggtgaa tttcgaccct ctagagggtc gaccctctag aggctgcagc cgaccccgga
     3661 tccggagctt ttagagcaga agtaacactt ccgtacaggc ctagaagtaa aggcaacatc
     3721 cactgaggag cagttctttg atttgcacca ccaggagcct caaatttttc aataaattca
     3781 cctgactgca cattaggact ttggctttga gcttcccacc tctcagttac ttgctgaata
     3841 ctgtctgctt catcaatatt atcataggtg tgcccaaatg atatttgcaa cccttccctg
     3901 ttggctactt gtctcaccat tgtaggccta atgggagaca aagtagagta gtaatcttgt
     3961 aaagagttat accaattaac aggagcatta attactgtcc aagtagtttc ctctaaaaac
     4021 cttgccaaac tgtcccttaa atatctttgg gttcttcttt caagctcctg tgaggtgagc
     4081 ctaggaatgt cattttgtat tacacgccaa aaagcttgag aaatggcatt aaaaagtgtt
     4141 ggaccccaat gtctggggtc aagatactga acactgtgaa caaaggtttg tactccagga
     4201 aataaaatat catagtaatc atctggccta tacaaatcta cagccattcc tggttgttga
     4261 tataaaccaa cagtagaaac tttgtgatcc cagtcactaa aaaatctata ccccacttga
     4321 gcaacagcgc tcacaccagt cacagtttgc agtaaagctg caaatccagc tatagcagca
     4381 ggagccccag atatcacagc ataggcctgt ggagtgaggc ctatagcagc aattgcctca
     4441 gaggttgtta ggccttcaac agtagcaaca gatgcaagtt gcacttcaat tgcagcagcg
     4501 gcctctccag cagcaatttc agctactgaa aatccagtag cagcagcagc ttcagacaca
     4561 gtagcaatta ggtcccccaa cagtgttaaa gcagcaccca tggacctgaa ataaaagaca
     4621 aaaagactaa acttaccagt taactttctg gtttttcagt taacctttct ggttttttgc
     4681 gtttcccgtc aacagtatct tccccttcac aaaattgcag caaaagctct aaaacaaaca
     4741 caaaaaggcg ttgagctgtt tttttacttt cagtccatga cctacgaacc ttaacggagg
     4801 cctggcgtga cagccggcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
     4861 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4921 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4981 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5041 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5101 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5161 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5221 ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     5281 aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga tgagaaaggc
     5341 atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag aaaaaatgaa
     5401 gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc atcaacctga
     5461 ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc ctggtgttga
     5521 tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta actgcatatg
     5581 cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga aagatccact
     5641 tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac ttgatctttg
     5701 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     5761 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     5821 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     5881 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     5941 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     6001 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     6061 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     6121 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
     6181 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
     6241 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
     6301 gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag catgatttta
     6361 atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa
     6421 tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa tttcagtaca
     6481 gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat aagtaccatg
     6541 aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa accatatgcc
     6601 aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa ttaactagag
     6661 aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata atgtttggtt
     6721 ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta cactgtttgt
     6781 tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac aacattccta
     6841 aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca ttagcagctg
     6901 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6961 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     7021 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     7081 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     7141 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     7201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8401 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     8461 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8521 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8581 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8641 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8701 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     8761 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8821 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8881 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8941 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9001 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9061 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9121 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     9181 cgtcttcaag aa
//
