LOCUS       pSV40-GLuc.        4178 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Control vector with a strong constitutive SV40 promoter for 
            expressing secreted Gaussia luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pSV40-GLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..45
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        51..246
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             306..860
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     polyA_signal    complement(898..1019)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1681..2269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2443..3300)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3301..3405)
                     /label=AmpR promoter
     rep_origin      3432..3887
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4018..4066
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4080..4171
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccaccg atcgatcgct agctgcagag ctcgaattcg atatcaagct tgcatctcaa
       61 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      121 ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag aggccgaggc
      181 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      241 ttgcaactcg agattccggt actgttggta aactaagctt ggtaccgagc tcggatccag
      301 ccaccatggg agtcaaagtt ctgtttgccc tgatctgcat cgctgtggcc gaggccaagc
      361 ccaccgagaa caacgaagac ttcaacatcg tggccgtggc cagcaacttc gcgaccacgg
      421 atctcgatgc tgaccgcggg aagttgcccg gcaagaagct gccgctggag gtgctcaaag
      481 agatggaagc caatgcccgg aaagctggct gcaccagggg ctgtctgatc tgcctgtccc
      541 acatcaagtg cacgcccaag atgaagaagt tcatcccagg acgctgccac acctacgaag
      601 gcgacaaaga gtccgcacag ggcggcatag gcgaggcgat cgtcgacatt cctgagattc
      661 ctgggttcaa ggacttggag cccatggagc agttcatcgc acaggtcgat ctgtgtgtgg
      721 actgcacaac tggctgcctc aaagggcttg ccaacgtgca gtgttctgac ctgctcaaga
      781 agtggctgcc gcaacgctgt gcgacctttg ccagcaagat ccagggccag gtggacaaga
      841 tcaagggggc cggtggtgac taagcggccg ctcgagcatg catctagaca gacatgataa
      901 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      961 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1021 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1081 aaagcaagta aaacctctac aaatgtggta ggatccgata aggatctgaa cgatggagcg
     1141 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1201 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     1261 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     1321 gggagcctgg ggactttcca caccctaact gacacacatt ccaggatccg tcgaccgatg
     1381 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     1441 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     1501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2101 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3421 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3481 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3721 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3781 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3841 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt
     3901 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
     3961 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
     4021 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     4081 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     4141 agtgcaagtg caggtgccag aacatttctc tatcgata
//
