LOCUS       pSV40-CLuc.        5256 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Control vector with a strong constitutive SV40 promoter for 
            expressing secreted Cypridina luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pSV40-CLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        51..246
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             291..1949
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     polyA_signal    complement(1976..2097)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2759..3347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3521..4378)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4379..4483)
                     /label=AmpR promoter
     rep_origin      4510..4965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5096..5144
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5158..5249
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccaccg atcgatcgct agctgcagag ctcgaattcg atatcaagct tgcatctcaa
       61 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      121 ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag aggccgaggc
      181 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      241 ttgcaactcg agattccggt actgttggta aactagtagg aggtagaacc atgaagacct
      301 taattcttgc cgttgcatta gtctactgcg ccactgttca ttgccaggac tgtccttacg
      361 aacctgatcc accaaacaca gttccaactt cctgtgaagc taaagaagga gaatgtattg
      421 atagcagctg tggcacctgc acgagagaca tactatcaga tggactgtgt gaaaataaac
      481 caggaaaaac atgttgccga atgtgtcagt atgtaattga atgcagagta gaggccgcag
      541 gatggtttag aacattctat ggaaagagat tccagttcca ggaacctggt acatacgtgt
      601 tgggtcaagg aaccaagggc ggcgactgga aggtgtccat caccctggag aacctggatg
      661 gaaccaaggg ggctgtgctg accaagacaa gactggaagt ggctggagac atcattgaca
      721 tcgctcaagc tactgagaat cccatcactg taaacggtgg agctgaccct atcatcgcca
      781 acccgtacac catcggcgag gtcaccatcg ctgttgttga gatgccaggc ttcaacatca
      841 ccgtcattga gttcttcaaa ctgatcgtga tcgacatcct cggaggaaga tctgtaagaa
      901 tcgccccaga cacagcaaac aaaggaatga tctctggcct ctgtggagat cttaaaatga
      961 tggaagatac agacttcact tcagatccag aacaactcgc tattcagcct aagatcaacc
     1021 aggagtttga cggttgtcca ctctatggaa atcctgatga cgttgcatac tgcaaaggtc
     1081 ttctggagcc gtacaaggac agctgccgca accccatcaa cttctactac tacaccatct
     1141 cctgcgcctt cgcccgctgt atgggtggag acgagcgagc ctcacacgtg ctgcttgact
     1201 acagggagac gtgcgctgct cccgaaacta gaggaacctg cgttttgtct ggacatactt
     1261 tctacgatac atttgacaaa gcaagatacc aattccaggg tccctgcaag gagattctta
     1321 tggccgccga ctgtttctgg aacacttggg atgtgaaggt ttcacacagg aatgttgact
     1381 cttacactga agtagagaaa gtacgaatca ggaaacaatc gactgtagta gaactcattg
     1441 ttgatggaaa acagattctg gttggaggag aagccgtgtc cgtcccgtac agctctcaga
     1501 acacttccat ctactggcaa gatggtgaca tactgactac agccatccta cctgaagctc
     1561 tggtggtcaa gttcaacttc aagcaactgc tcgtcgtaca tattagagat ccattcgatg
     1621 gtaagacttg cggtatttgc ggtaactaca accaggattt cagtgatgat tcttttgatg
     1681 ctgaaggagc ctgtgatctg acccccaacc caccgggatg caccgaagaa cagaaacctg
     1741 aagctgaacg actctgcaat agtctcttcg ccggtcaaag tgatcttgat cagaaatgta
     1801 acgtgtgcca caagcctgac cgtgtcgaac gatgcatgta cgagtattgc ctgaggggac
     1861 aacagggttt ctgtgaccac gcatgggagt tcaagaaaga atgctacata aagcatggag
     1921 acaccctaga agtaccagat gaatgcaaat agacgcgtac tctagacaga catgataaga
     1981 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2041 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2101 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2161 agcaagtaaa acctctacaa atgtggtagg atccgataag gatctgaacg atggagcgga
     2221 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     2281 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     2341 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     2401 gagcctgggg actttccaca ccctaactga cacacattcc aggatccgtc gaccgatgcc
     2461 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     2521 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     2581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2941 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3181 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4501 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4921 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca
     4981 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca
     5041 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa
     5101 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     5161 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     5221 tgcaagtgca ggtgccagaa catttctcta tcgata
//
