LOCUS       40924_41729        5729 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSV2neo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Southern PJ, Berg P.
  TITLE     Transformation of mammalian cells to antibiotic resistance with a 
            bacterial gene under control of the SV40 early region promoter
  JOURNAL   J. Mol. Appl. Genet. 1 (4), 327-341 (1982)
  PUBMED    6286831
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5729)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 5729)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. 
            Appl. Genet."; date: "1982"; volume: "1"; issue: "4"; pages: 
            "327-341"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH; this vector has not been 
            completely sequenced. If you suspect there is an error in this 
            sequence, please contact CLONTECH's Technical Service Department at 
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail 
            TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..5729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(752..886)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1311..1331)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     intron          complement(1461..1526)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1950..2741)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3098..3427)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    3578..3718
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3904..4492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4666..5523)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5524..5628)
                     /label=AmpR promoter
ORIGIN
        1 aattcctttg cctaatttaa atgaggactt aacctgtgga aatattttga tgtgggaagc
       61 tgttactgtt aaaactgagg ttattggggt aactgctatg ttaaacttgc attcagggac
      121 acaaaaaact catgaaaatg gtgctggaaa acccattcaa gggtcaaatt ttcatttttt
      181 tgctgttggt ggggaacctt tggagctgca gggtgtgtta gcaaactaca ggaccaaata
      241 tcctgctcaa actgtaaccc caaaaaatgc tacagttgac agtcagcaga tgaacactga
      301 ccacaaggct gttttggata aggataatgc ttatccagtg gagtgctggg ttcctgatcc
      361 aagtaaaaat gaaaacacta gatattttgg aacctacaca ggtggggaaa atgtgcctcc
      421 tgttttgcac attactaaca cagcaaccac agtgcttctt gatgagcagg gtgttgggcc
      481 cttgtgcaaa gctgacagct tgtatgtttc tgctgttgac atttgtgggc tgtttaccaa
      541 cacttctgga acacagcagt ggaagggact tcccagatat tttaaaatta cccttagaaa
      601 gcggtctgtg aaaaacccct acccaatttc ctttttgtta agtgacctaa ttaacaggag
      661 gacacagagg gtggatgggc agcctatgat tggaatgtcc tctcaagtag aggaggttag
      721 ggtttatgag gacacagagg agcttcctgg ggatccagac atgataagat acattgatga
      781 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      841 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
      901 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      961 cctctacaaa tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat
     1021 tccttattaa cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta
     1081 ttaatagcag acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa
     1141 ctgttatgcc tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt
     1201 gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca
     1261 gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc
     1321 ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc
     1381 atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca
     1441 tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat
     1501 tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc
     1561 aattatgtca caccacagaa gtaaggttcc ttcacaaaga tccgggacca aagcggccat
     1621 cgtgcctccc cactcctgca gttcgggggc atggatgcgc ggatagccgc tgctggtttc
     1681 ctggatgccg acggatttgc actgccggta gaactccgcg aggtcgtcca gcctcaggca
     1741 gcagctgaac caactcgcga ggggatcgag cccggggtgg gcgaagaact ccagcatgag
     1801 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     1861 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     1921 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     1981 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     2041 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     2101 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     2161 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     2221 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     2281 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     2341 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     2401 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     2461 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     2521 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     2581 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     2641 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     2701 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     2761 gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     2821 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     2881 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
     2941 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
     3001 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
     3061 cccttgcgcc ctgagtgctt gcggcagcgt gaagcttttt gcaaaagcct aggcctccaa
     3121 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
     3181 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
     3241 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt
     3301 gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga
     3361 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact
     3421 gacacacatt ccacagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     3481 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     3541 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     3601 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     3661 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     3721 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3781 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3841 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3901 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3961 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4021 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4081 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4141 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4201 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4261 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4321 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4381 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4441 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4501 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4561 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4621 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4681 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4741 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4801 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4861 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4921 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
     4981 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5041 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5101 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5161 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5221 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     5281 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5341 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5401 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5461 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5521 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5581 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5641 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5701 gcgtatcacg aggccctttc gtcttcaag
//
