LOCUS       40924_41724        5487 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV25ShTNFR75m5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5487)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5487)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87717.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     CDS             complement(1620..2828)
                     /codon_start=1
                     /note="unnamed protein product; R75m5"
                     /protein_id="SJL87718.1"
                     /translation="MPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFC
                     TKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYC
                     ALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQIC
                     NVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLP
                     MGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREAKVPHL
                     PADKARGTQGPEQQHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEASGAGEAR
                     ASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTDSSPSESPKDEQV
                     PFS"
     misc_feature    complement(2829..2894)
                     /label=sTNFR
                     /note="sTNFR"
     regulatory      2891..2900
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2916..3245)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3660..4248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4422..5279)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5280..5384)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtacct
     1621 taggagaagg ggacctgctc gtccttcggg gactccgagg ggctggaatc tgtgtctccc
     1681 attgtggagc tggcttggga ggagcactgt gagctgtggt cagagctgct acagacgttc
     1741 acgatgcagg tgacattgac ctgggtccca tggccaccag gggaagaatc tgagctcccg
     1801 gtgctggccc gggcctcccc ggccccactg gcctccacgc ctggtgcctg tggctggttc
     1861 cgagtgggcg cccttctgtc caacgcactg gccgagctct ccagggagct gctgctggag
     1921 ctcggcgctg tgatcagcag gtgctgctgc tcggggccct gtgtaccccg ggccttatcg
     1981 gcaggcaagt gaggcacctt ggcttctctc tgcaggcaca agggcttctt tttcacctgg
     2041 gtcatgatga cacagttcac cactcctatt attagtagac ccaaggctgt cacacccaca
     2101 atcagtccaa ctggaagagc gaagtcgcca gtgctccctt cagctggggg gctggggccc
     2161 attgggagca ggaaggaggt gcttggagca gtgctgggtt ctggagttgg ctgcgtgtgt
     2221 tgggatcgtg tggacactgg ctggggtaag tgtactgccc ctggggccat actccgggtg
     2281 ggggacgtgg acgtgcagac tgcatccatg cttgcattcc cagggatggc caccacgtta
     2341 cagatctggt ggggcctgca aatatccgtg gatgaagtcg tgttggagaa cgtccccggg
     2401 gcacagggct tgcacaccac gtctgatgtt tcagttcctg gtctggccac gccgaagccc
     2461 gggcggcact tgcgcagcgg cgcgcacagc cggcacccct cctgcttgct cagcgcgcag
     2521 taccagccgg gcctgcaggt gcagatgcgg ttctgttccc gagtgcaggc ttgagtttcc
     2581 acctggtcag agctacagcg ggagccacag ctcaagcact cgggaaccca gttccagagc
     2641 tgggtgtatg tgctgtcctc acaggagtca cacacggtgt ccgaggtctt ggtacagaag
     2701 acttttgcat gttggcccgg cgagcatttg ctgcagcaca tctgagctgt ctggtcatag
     2761 tattctctga gccggcatgt gctcccgggc tccggggcgt agggtgtaaa tgccacctgg
     2821 gcgggcaagg cgtgcgccgc agcccagagc tccagtccga cggccagcgc ggcccagacg
     2881 gcgacgggcg ccatggtggc ggcaagcttg agctttttgc aaaagcctag gcctccaaaa
     2941 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     3001 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     3061 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     3121 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     3181 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     3241 cacacattcc acagtcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
     3301 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     3361 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     3421 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgctct
     3481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4081 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4381 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4441 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4501 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4561 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4621 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4681 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     4741 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4801 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4861 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4921 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4981 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     5041 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5101 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5161 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5221 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5281 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5341 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5401 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5461 atcacgaggc cctttcgtct tcaagaa
//
