LOCUS       40924_41709        5598 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV25ShTNFR75m2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5598)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5598)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87705.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     CDS             complement(2127..2939)
                     /codon_start=1
                     /note="unnamed protein product; R75m2"
                     /protein_id="SJL87706.1"
                     /translation="MPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFC
                     TKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYC
                     ALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQIC
                     NVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLP
                     MGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPL"
     misc_feature    complement(2940..3005)
                     /label=sTNFR
                     /note="sTNFR"
     regulatory      3002..3011
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3027..3356)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3771..4359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4533..5390)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5391..5495)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtacct
     1621 taactgggct tcatcccagc atcaggcact ccaaggggca ggggcttctc ttcggtgctc
     1681 cccagcaggg tctctggcgt ctccagctgt gaccgaaagg cacattcctc cttggagaag
     1741 gggacctgct cgtccttcgg ggactccgag gggctggaat ctgtgtctcc cattgtggag
     1801 ctggcttggg aggagcactg tgagctgtgg tcagagctgc tacagacgtt cacgatgcag
     1861 gtgacattga cctgggtccc atggccacca ggggaagaat ctgagctccc ggtgctggcc
     1921 cgggcctccc cggccccact ggcctccacg cctggtgcct gtggctggtt ccgagtgggc
     1981 gcccttctgt ccaacgcact ggccgagctc tccagggagc tgctgctgga gctcggcgct
     2041 gtgatcagca ggtgctgctg ctcggggccc tgtgtacccc gggccttatc ggcaggcaag
     2101 tgaggcacct tggcttctct ctgctatcac aagggcttct ttttcacctg ggtcatgatg
     2161 acacagttca ccactcctat tattagtaga cccaaggctg tcacacccac aatcagtcca
     2221 actggaagag cgaagtcgcc agtgctccct tcagctgggg ggctggggcc cattgggagc
     2281 aggaaggagg tgcttggagc agtgctgggt tctggagttg gctgcgtgtg ttgggatcgt
     2341 gtggacactg gctggggtaa gtgtactgcc cctggggcca tactccgggt gggggacgtg
     2401 gacgtgcaga ctgcatccat gcttgcattc ccagggatgg ccaccacgtt acagatctgg
     2461 tggggcctgc aaatatccgt ggatgaagtc gtgttggaga acgtccccgg ggcacagggc
     2521 ttgcacacca cgtctgatgt ttcagttcct ggtctggcca cgccgaagcc cgggcggcac
     2581 ttgcgcagcg gcgcgcacag ccggcacccc tcctgcttgc tcagcgcgca gtaccagccg
     2641 ggcctgcagg tgcagatgcg gttctgttcc cgagtgcagg cttgagtttc cacctggtca
     2701 gagctacagc gggagccaca gctcaagcac tcgggaaccc agttccagag ctgggtgtat
     2761 gtgctgtcct cacaggagtc acacacggtg tccgaggtct tggtacagaa gacttttgca
     2821 tgttggcccg gcgagcattt gctgcagcac atctgagctg tctggtcata gtattctctg
     2881 agccggcatg tgctcccggg ctccggggcg tagggtgtaa atgccacctg ggcgggcaag
     2941 gcgtgcgccg cagcccagag ctccagtccg acggccagcg cggcccagac ggcgacgggc
     3001 gccatggtgg cggcaagctt gagctttttg caaaagccta ggcctccaaa aaagcctcct
     3061 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     3121 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     3181 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     3241 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
     3301 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
     3361 cacagtcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga
     3421 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
     3481 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
     3541 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc
     3601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3661 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4201 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     4861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     5161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5401 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5461 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5521 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     5581 ccctttcgtc ttcaagaa
//
