LOCUS       40924_41704        5613 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV25ShTNFR75m1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5613)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5613)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87701.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     CDS             complement(1998..2954)
                     /codon_start=1
                     /note="unnamed protein product; R75m1"
                     /protein_id="SJL87702.1"
                     /translation="MPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFC
                     TKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYC
                     ALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQIC
                     NVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLP
                     MGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREAKVPHL
                     PADKARGTQGPEQQHLLITAPSSSSSSLESSATSRGL"
     misc_feature    complement(2955..3020)
                     /label=sTNFR
                     /note="sTNFR"
     regulatory      3017..3026
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3042..3371)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3786..4374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4548..5405)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5406..5510)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtacct
     1621 taactgggct tcatcccagc atcaggcact ccaaggggca ggggcttctc ttcggtgctc
     1681 cccagcaggg tctctggcgt ctccagctgt gaccgaaagg cacattcctc cttggagaag
     1741 gggacctgct cgtccttcgg ggactccgag gggctggaat ctgtgtctcc cattgtggag
     1801 ctggcttggg aggagcactg tgagctgtgg tcagagctgc tacagacgtt cacgatgcag
     1861 gtgacattga cctgggtccc atggccacca ggggaagaat ctgagctccc ggtgctggcc
     1921 cgggcctccc cggccccact ggcctccacg cctggtgcct gtggctggtt ccgagtgggc
     1981 gcccttctgt ccaacctcta gaggcctcta gaggtggccg agctctccag ggagctgctg
     2041 ctggagctcg gcgctgtgat cagcaggtgc tgctgctcgg ggccctgtgt accccgggcc
     2101 ttatcggcag gcaagtgagg caccttggct tctctctgca ggcacaaggg cttctttttc
     2161 acctgggtca tgatgacaca gttcaccact cctattatta gtagacccaa ggctgtcaca
     2221 cccacaatca gtccaactgg aagagcgaag tcgccagtgc tcccttcagc tggggggctg
     2281 gggcccattg ggagcaggaa ggaggtgctt ggagcagtgc tgggttctgg agttggctgc
     2341 gtgtgttggg atcgtgtgga cactggctgg ggtaagtgta ctgcccctgg ggccatactc
     2401 cgggtggggg acgtggacgt gcagactgca tccatgcttg cattcccagg gatggccacc
     2461 acgttacaga tctggtgggg cctgcaaata tccgtggatg aagtcgtgtt ggagaacgtc
     2521 cccggggcac agggcttgca caccacgtct gatgtttcag ttcctggtct ggccacgccg
     2581 aagcccgggc ggcacttgcg cagcggcgcg cacagccggc acccctcctg cttgctcagc
     2641 gcgcagtacc agccgggcct gcaggtgcag atgcggttct gttcccgagt gcaggcttga
     2701 gtttccacct ggtcagagct acagcgggag ccacagctca agcactcggg aacccagttc
     2761 cagagctggg tgtatgtgct gtcctcacag gagtcacaca cggtgtccga ggtcttggta
     2821 cagaagactt ttgcatgttg gcccggcgag catttgctgc agcacatctg agctgtctgg
     2881 tcatagtatt ctctgagccg gcatgtgctc ccgggctccg gggcgtaggg tgtaaatgcc
     2941 acctgggcgg gcaaggcgtg cgccgcagcc cagagctcca gtccgacggc cagcgcggcc
     3001 cagacggcga cgggcgccat ggtggcggca agcttgagct ttttgcaaaa gcctaggcct
     3061 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     3121 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     3181 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     3241 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     3301 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     3361 aactgacaca cattccacag tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     3421 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     3481 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     3541 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
     3601 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3661 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3721 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3781 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3841 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3901 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3961 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4021 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4081 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4141 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4201 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     4261 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4321 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4381 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4441 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4501 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4561 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4621 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4681 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4741 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4801 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4861 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4921 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4981 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5041 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5101 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5161 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5221 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5281 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5341 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5401 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5461 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5521 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     5581 aggcgtatca cgaggccctt tcgtcttcaa gaa
//
