LOCUS       V002971                 5256 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002971
VERSION     V002971
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(754..888)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1313..1333)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1463..1528)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1530..1604)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88614.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1612..1851)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(1869..1913)
                     /codon_start=1
                     /note="unnamed protein product; rPDIf"
                     /protein_id="SJL88615.1"
                     /translation="MEEDDDQKAVKDEL"
     CDS             complement(1920..2624)
                     /gene="Tnf"
                     /label="Tumor necrosis factor"
                     /note="Tumor necrosis factor from Mus musculus. Accession#:
                     P06804"
     misc_feature    complement(2625..2652)
                     /label="5UTR"
                     /note="5UTR"
     promoter        complement(2685..3014)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3429..4017)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4191..5048)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5049..5153)
                     /label="AmpR promoter"
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atcgatccag acatgataag atacattgat
      781 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      841 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      901 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      961 aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat
     1021 attccttatt aaccccttta caaattaaaa agctaaaggt acacaatttt tgagcatagt
     1081 tattaatagc agacactcta tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat
     1141 aactgttatg cctacttata aaggttacag aatatttttc cataattttc ttgtatagca
     1201 gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc aagagttcta ttactaaaca
     1261 cagcatgact caaaaaactt agcaattctg aaggaaagtc cttggggtct tctacctttc
     1321 tcttcttttt tggaggagta gaatgttgag agtcagcagt agcctcatca tcactagatg
     1381 gcatttcttc tgagcaaaac aggttttcct cattaaaggc attccaccac tgctcccatt
     1441 catcagttcc ataggttgga atctaaaata cacaaacaat tagaatcagt agtttaacac
     1501 attatacact taaaaatttt atatttacct tagagcttta aatctctgta ggtagtttgt
     1561 ccaattatgt cacaccacag aagtaaggtt ccttcacaaa gatccgtcga cnnnnnggaa
     1621 agcccatttg agtccttgat ggtggtgcat gagaggccca cagtccaggt cactgtccca
     1681 gcatcttgtg tttctgagta gttgttgaaa gctctgagca cagagttgga ctctgagcca
     1741 taatcccctt tctaagttag aaggacacag actgggggct ctgaggagta gacaataaag
     1801 gggtcagagt aaaggggtca gagtgggggc tgggtagaga atggatgaac acggatctgg
     1861 cttctgcact acagttcatc cttcacggct ttctgatcgt cgtcttcttc cattctagac
     1921 agagcaatga ctccaaagta gacctgcccg gactccgcaa agtctaagta cttgggcaga
     1981 ttgacctcag cgctgagttg gtcccccttc tccagctgga agactcctcc caggtatatg
     2041 ggctcatacc agggtttgag ctcagccccc tcaggggtgt ccttggggca ggggctcttg
     2101 acggcagaga ggaggttgac tttctcctgg tatgagatag caaatcggct gacggtgtgg
     2161 gtgaggagca cgtagtcggg gcagccttgt cccttgaaga gaacctggga gtagacaagg
     2221 tacaacccat cggctggcac cactagttgg ttgtctttga gatccatgcc gttggccagg
     2281 agggcgttgg cgcgctggct cagccactcc agctgctcct ccacttggtg gtttgctacg
     2341 acgtgggcta caggcttgtc actcgaattt tgagaagatg atctgagtgt gagggtctgg
     2401 gccatagaac tgatgagagg gaggccattt gggaacttct catccctttg gggaccgatc
     2461 accccgaagt tcagtagaca gaagagcgtg gtggcccctg ccacaagcag gaatgagaag
     2521 aggctgagac ataggcaccg cctggagttc tggaagcccc ccatcttttg ggggagtgcc
     2581 tcttctgcca gttccacgtc gcggatcatg ctttctgtgc tcatggtgtc ttttctggag
     2641 ggagatgtgg cgcgtcgact ctagctagag ccaagcttga gctttttgca aaagcctagg
     2701 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     2761 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     2821 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     2881 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     2941 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     3001 cctaactgac acacattcca cagtcgggca gcgttgggtc ctggccacgg gtgcgcatga
     3061 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
     3121 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
     3181 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
     3241 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3421 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3601 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3841 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3901 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3961 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4321 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4501 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4801 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5161 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     5221 aataggcgta tcacgaggcc ctttcgtctt caagaa
//
