LOCUS       40924_41644        5061 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV23SmTNFd1-9K11E, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5061)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5061)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87564.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1624..1781)
                     /label=3' UTR of mTNF
                     /note="3' UTR of mTNF"
     CDS             complement(1782..2225)
                     /codon_start=1
                     /note="unnamed protein product; mature mTNFd1-9K11E"
                     /protein_id="SJL87565.1"
                     /translation="DEPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADG
                     LYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPW
                     YEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL"
     CDS             complement(2226..2462)
                     /codon_start=1
                     /note="unnamed protein product; presequence of mTNF"
                     /protein_id="SJL87566.1"
                     /translation="MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGAT
                     TLFCLLNFGVIGPQRDEKFPNGLPLISSMAQTLT"
     misc_feature    complement(2463..2490)
                     /label=5' UTR of mTNF
                     /note="5' UTR of mTNF"
     promoter        complement(2509..2806)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3234..3822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3996..4853)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4854..4958)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgaccgg gggggggggg
     1621 ggggttgaaa gctctgagca cagagttgga ctctgagcca taatcccctt tctaagttag
     1681 aaggacacag actgggggct ctgaggagta gacaataaag gggtcagagt aaaggggtca
     1741 gagtgggggc tgggtagaga atggatgaac acccattccc ttcacagagc aatgactcca
     1801 aagtagacct gcccggactc cgcaaagtct aagtacttgg gcagattgac ctcagcgctg
     1861 agttggtccc ccttctccag ctggaagact cctcccaggt atatgggctc ataccagggt
     1921 ttgagctcag ccccctcagg ggtgtccttg gggcaggggc tcttgacggc agagaggagg
     1981 ttgactttct cctggtatga gatagcaaat cggctgacgg tgtgggtgag gagcacgtag
     2041 tcggggcagc cttgtccctt gaagagaacc tgggagtaga caaggtacaa cccatcggct
     2101 ggcaccacta gttggttgtc tttgagatcc atgccgttgg ccaggagggc gttggcgcgc
     2161 tggctcagcc actccagctg ctcctccact tggtggtttg ctacgacgtg cgcaacaggc
     2221 tcgtctgtga gggtctgggc catagaactg atgagaggga ggccatttgg gaacttctca
     2281 tccctttggg gaccgatcac cccgaagttc agtagacaga agagcgtggt ggcccctgcc
     2341 acaagcagga atgagaagag gctgagacat aggcaccgcc tggagttctg gaagcccccc
     2401 atcttttggg ggagtgcctc ttctgccagt tccacgtcgc ggatcatgct ttctgtgctc
     2461 atggtgtctt ttctggaggg agatgtggcg ggtcgacgga tcccccctcc aaaaaagcct
     2521 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     2581 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     2641 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     2701 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     2761 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     2821 ttccacagtc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     2881 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     2941 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     3001 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ctcttccgct
     3061 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3661 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     4321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     4621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4981 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     5041 aggccctttc gtcttcaaga a
//
