LOCUS       40924_41639        5137 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV23SmTNFd1-9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5137)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5137)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88593.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1608..1857)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(1858..2301)
                     /codon_start=1
                     /note="unnamed protein product; TNFd"
                     /protein_id="SJL88594.1"
                     /translation="DKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADG
                     LYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPW
                     YEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL"
     misc_feature    complement(2302..2538)
                     /label=pmTNF
                     /note="pmTNF"
     misc_feature    complement(2539..2566)
                     /label=5UTR
                     /note="5UTR"
     promoter        complement(2585..2882)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3310..3898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4072..4929)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4930..5034)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgacnnn nnggaaagcc
     1621 catttgagtc cttgatggtg gtgcatgaga ggcccacagt ccaggtcact gtcccagcat
     1681 cttgtgtttc tgagtagttg ttgaaagctc tgagcacaga gttggactct gagccataat
     1741 cccctttcta agttagaagg acacagactg ggggctctga ggagtagaca ataaaggggt
     1801 cagagtaaag gggtcagagt gggggctggg tagagaatgg atgaacaccc attcccttca
     1861 cagagcaatg actccaaagt agacctgccc ggactccgca aagtctaagt acttgggcag
     1921 attgacctca gcgctgagtt ggtccccctt ctccagctgg aagactcctc ccaggtatat
     1981 gggctcatac cagggtttga gctcagcccc ctcaggggtg tccttggggc aggggctctt
     2041 gacggcagag aggaggttga ctttctcctg gtatgagata gcaaatcggc tgacggtgtg
     2101 ggtgaggagc acgtagtcgg ggcagccttg tcccttgaag agaacctggg agtagacaag
     2161 gtacaaccca tcggctggca ccactagttg gttgtctttg agatccatgc cgttggccag
     2221 gagggcgttg gcgcgctggc tcagccactc cagctgctcc tccacttggt ggtttgctac
     2281 gacgtgggct acaggcttgt ctgtgagggt ctgggccata gaactgatga gagggaggcc
     2341 atttgggaac ttctcatccc tttggggacc gatcaccccg aagttcagta gacagaagag
     2401 cgtggtggcc cctgccacaa gcaggaatga gaagaggctg agacataggc accgcctgga
     2461 gttctggaag ccccccatct tttgggggag tgcctcttct gccagttcca cgtcgcggat
     2521 catgctttct gtgctcatgg tgtcttttct ggagggagat gtggcgcgtc gacggatccc
     2581 ccctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     2641 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     2701 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     2761 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     2821 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     2881 ccctaactga cacacattcc acagtcgggc agcgttgggt cctggccacg ggtgcgcatg
     2941 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
     3001 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
     3061 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
     3121 tcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3721 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4381 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4681 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5041 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     5101 aaataggcgt atcacgaggc cctttcgtct tcaagaa
//
