LOCUS       40924_41664        5193 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV23SMTNFm2t, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5193)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5193)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88767.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1608..1857)
                     /label=3UTR
                     /note="3UTR"
     misc_feature    complement(1858..2328)
                     /label=mTNFm
                     /note="mTNFm"
     CDS             complement(2329..2565)
                     /codon_start=1
                     /note="unnamed protein product; pmTNF"
                     /protein_id="SJL88768.1"
                     /translation="MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGAT
                     TLFCLLNFGVIGPQRDEKFPNGLPLISSMAQTLT"
     misc_feature    complement(2566..2593)
                     /label=5UTR
                     /note="5UTR"
     promoter        complement(2622..2951)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3366..3954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4128..4985)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4986..5090)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgacnnn nnggaaagcc
     1621 catttgagtc cttgatggtg gtgcatgaga ggcccacagt ccaggtcact gtcccagcat
     1681 cttgtgtttc tgagtagttg ttgaaagctc tgagcacaga gttggactct gagccataat
     1741 cccctttcta agttagaagg acacagactg ggggctctga ggagtagaca ataaaggggt
     1801 cagagtaaag gggtcagagt gggggctggg tagagaatgg atgaacaccc attcccttca
     1861 cagagcaatg actccaaagt agacctgccc ggactccgca aagtctaagt acttgggcag
     1921 attgacctca gcgctgagtt ggtccccctt ctccagctgg aagactcctc ccaggtatat
     1981 gggctcatac cagggtttga gctcagcccc ctcaggggtg tccttggggc aggggctctt
     2041 gacggcagag aggaggttga ctttctcctg gtatgagata gcaaatcggc tgacggtgtg
     2101 ggtgaggagc acgtagtcgg ggcagccttg tcccttgaag agaacctggg agtagacaag
     2161 gtacaaccca tcggctggca ccactagttg gttgtctttg agatccatgc cgttggccag
     2221 gagggcgttg gcgcgctggc tcagccactc cagctgctcc tccacttggt ggtttgctac
     2281 gacgtgggct acaggcttgt cactcgaatt ttgagactat gatcagagtg tgagggtctg
     2341 ggccatagaa ctgatgagag ggaggccatt tgggaacttc tcatcccttt ggggaccgat
     2401 caccccgaag ttcagtagac agaagagcgt ggtggcccct gccacaagca ggaatgagaa
     2461 gaggctgaga cataggcacc gcctggagtt ctggaagccc cccatctttt gggggagtgc
     2521 ctcttctgcc agttccacgt cgcggatcat gctttctgtg ctcatggtgt cttttctgga
     2581 gggagatgtg gcgcgtcgac tctagagcca agcttgagct ttttgcaaaa gcctaggcct
     2641 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     2701 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     2761 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     2821 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     2881 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     2941 aactgacaca cattccacag tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     3001 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     3061 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     3121 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
     3181 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3241 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3301 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3541 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3781 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3901 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3961 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4021 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4081 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4141 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4201 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4261 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4321 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4381 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4441 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4501 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4561 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4621 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4681 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4741 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4801 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4861 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4921 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4981 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5041 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5101 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     5161 aggcgtatca cgaggccctt tcgtcttcaa gaa
//
