LOCUS       V002981                 5117 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002981
VERSION     V002981
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5117)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5117)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL89020.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1624..1781)
                     /label="3' UTR of mTNF"
                     /note="3' UTR of mTNF"
     CDS             complement(1785..2489)
                     /gene="Tnf"
                     /label="Tumor necrosis factor"
                     /note="Tumor necrosis factor from Mus musculus. Accession#:
                     P06804"
     misc_feature    complement(2490..2517)
                     /label="5' UTR of mTNF"
                     /note="5' UTR of mTNF"
     promoter        complement(2546..2875)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3290..3878)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4052..4909)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4910..5014)
                     /label="AmpR promoter"
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgaccgg gggggggggg
     1621 ggggttgaaa gctctgagca cagagttgga ctctgagcca taatcccctt tctaagttag
     1681 aaggacacag actgggggct ctgaggagta gacaataaag gggtcagagt aaaggggtca
     1741 gagtgggggc tgggtagaga atggatgaac acccattccc ttcacagagc aatgactcca
     1801 aagtagacct gcccggactc cgcaaagtct aagtacttgg gcagattgac ctcagcgctg
     1861 agttggtccc ccttctccag ctggaagact cctcccaggt atatgggctc ataccagggt
     1921 ttgagctcag ccccctcagg ggtgtccttg gggcaggggc tcttgacggc agagaggagg
     1981 ttgactttct cctggtatga gatagcaaat cggctgacgg tgtgggtgag gagcacgtag
     2041 tcggggcagc cttgtccctt gaagagaacc tgggagtaga caaggtacaa cccatcggct
     2101 ggcaccacta gttggttgtc tttgagatcc atgccgttgg ccaggagggc gttggcgcgc
     2161 tggctcagcc actccagctg ctcctccact tggtggtttg ctacgacgtg ggctacaggc
     2221 ttgtcactcg aattttgaga agatgatctg agtgtgaggg tctgggccat agaactgatg
     2281 agagggaggc catttgggaa cttctcatcc ctttggggac cgatcacccc gaagttcagt
     2341 agacagaaga gcgtggtggc ccctgccaca agcaggaatg agaagaggct gagacatagg
     2401 caccgcctgg agttctggaa gccccccatc ttttggggga gtgcctcttc tgccagttcc
     2461 acgtcgcgga tcatgctttc tgtgctcatg gtgtcttttc tggagggaga tgtggcgggt
     2521 cgactctaga gccaagcttg agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     2581 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     2641 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     2701 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     2761 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     2821 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     2881 acagtcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     2941 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
     3001 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     3061 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgctct tccgcttcct
     3121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3721 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     4381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     4681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5041 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     5101 cctttcgtct tcaagaa
//
