LOCUS       V002985                 5404 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002985
VERSION     V002985
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5404)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL89029.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1609..1924)
                     /label="3' UTR of hTNF"
                     /note="3' UTR of hTNF"
     CDS             complement(1928..2626)
                     /gene="TNF"
                     /label="Tumor necrosis factor"
                     /note="Tumor necrosis factor from Homo sapiens. Accession#:
                     P01375"
     misc_feature    complement(2627..2774)
                     /label="5' UTR of hTNF"
                     /note="5' UTR of hTNF"
     promoter        complement(2833..3162)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3577..4165)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4339..5196)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5197..5301)
                     /label="AmpR promoter"
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgacggc attctggcca
     1621 gaaccaaagg ctccctggtc tccagattcc agatgtcagg gatcaaagct gtaggcccca
     1681 gtgagttctg gaggccccag tttgaattct tagtggttgc cagcaattca ctgtgcaggc
     1741 cacacattcc tgaatcccag gtttcgaagt ggtggtcttg ttgcttaaag ttctaagctt
     1801 gggttccgac cctaagcccc caattctctt tttgagccag aagaggttga gggtgtctga
     1861 aggagggggt aataaaggga ttggggcagg ggaggcgttt gggaaggttg gatgttcgtc
     1921 ctcctcacag ggcaatgatc ccaaagtaga cctgcccaga ctcggcaaag tcgagatagt
     1981 cgggccgatt gatctcagcg ctgagtcggt cacccttctc cagctggaag acccctccca
     2041 gatagatggg ctcataccag ggcttggcct cagccccctc tggggtctcc ctctggcagg
     2101 ggctcttgat ggcagagagg aggttgacct tggtctggta ggagacggcg atgcggctga
     2161 tggtgtgggt gaggagcaca tgggtggagg ggcagccttg gcccttgaag aggacctggg
     2221 agtagatgag gtacaggccc tctgatggca ccaccagctg gttatctctc agctccacgc
     2281 cattggccag gagggcattg gcccggcggt tcagccactg gagctgcccc tcagcttgag
     2341 ggtttgctac aacatgggct acaggcttgt cactcggggt tcgagaagat gatctgactg
     2401 cctgggccag agggctgatt agagagaggt ccctggggaa ctcttccctc tgggggccga
     2461 tcactccaaa gtgcagcagg cagaagagcg tggtggcgcc tgccacgatc aggaaggaga
     2521 agaggctgag gaacaagcac cgcctggagc cctggggccc ccctgtcttc ttggggagcg
     2581 cctcctcggc cagctccacg tcccggatca tgctttcagt gctcatggtg tcctttccag
     2641 gggagagagg gtggagccgt gggtcagtat gtgagaggaa gagaacctgc ctggcagctt
     2701 gtcaggggat gtggcgtctg agggttgttt tcaggggggg tctgtagttg cttctctccc
     2761 tcttagctgg tcctcccccc cccccccccc cccccccccc cccccgtcga ctctagagcc
     2821 aagcttgagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     2881 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     2941 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     3001 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     3061 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     3121 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gtcgggcagc
     3181 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     3241 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     3301 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     3361 gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc gcttcctcgc tcactgactc
     3421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4441 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4621 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     4681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4921 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     4981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5161 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5281 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     5341 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     5401 agaa
//
