LOCUS       40924_41604        5724 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV23SE6HmRK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88194.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1613..1703)
                     /label=3' UTR of mIghg2b
                     /note="3' UTR of mIghg2b"
     CDS             complement(1707..2714)
                     /label=mlgG2b-CH
                     /note="Mouse IgG2b heavy chain constant region"
     promoter        complement(3153..3482)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3897..4485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4659..5516)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5517..5621)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcaggcttt
     1621 gctggatgct ttatttatac aagggatgca tggatatggg tgccagtgtc tcttaggacc
     1681 tgagagcttt gtgggtgctg agctcattta cccggagacc gggagatggt cttcttcagg
     1741 tagtaatttt tcagaccctc gtgtctcacg ttgcatgaga aggaatctgt tttctcccac
     1801 ttgcttgttt tcatattgag cttgctacgt acgaagtaag aaccgtcaga gtctaggact
     1861 ggtgcggtgt ccttgtagtt ctcctctgta tgcccattgc tggtccactc cacactgatg
     1921 tcttcagggc tgaagcccac ggccaggcaa gtcagactga catctttcct ggacaactgc
     1981 tctggtggtg gcgacaagat gtatacttgt ggagctctga ctatcccttt aatttttgag
     2041 atggttctct caatgggcgc tgggaggtct ttgttgttga ccttgcattt gaactccttg
     2101 ccactcatcc agtcctggtg ctggatgggg agggcactga ccacccggat agtactgttg
     2161 taatcctctc tatgggtttg tgtctgagct gtgagtactt ccacgttgtt cacaaaccag
     2221 ctgatctgga cgtctgggtc atcctcgctc acatccacca ccacacacgt gaccttgggt
     2281 gtcagggaga tcatgagtac atccttgata tttggaggga agatgaagac ggatggtcca
     2341 ccctcgaggt taggagctgg gcatttgtga cactccttgc atggaggaca ggggttgatt
     2401 gttgaagtgg gcccgctggg ctcaagtttt ttgtccaccg tggtgctgct ggctgggtga
     2461 gcaacactgc aggtaacggt ctggcttggc caggtgctgg aggggacagt cactgagctg
     2521 ctcatagtgt agagtccaga ctgcaggaga gctgggaagg tgtgcacact gctggacagg
     2581 gatccggagt tccaagtcac agtcactgac tcagggaagt agcccttgac caggcatccc
     2641 agagtcacgg aggaaccagt tgtatctcca cacccagggg ccagtggata gactgatggg
     2701 ggtgttgttt tggctgcaga ggcagtgacc agagtccctt ggccccagta accaaaaggg
     2761 agtagcccga cctgtctagg tccggcacag aaatagacgg cagagtcctc agatgtcagg
     2821 ctgctgaggt gcaagtaggc tgtgctggag gatttgtcta cagtcagtgt ggccttgccc
     2881 ttaaacttct cattgaagta cgaatttcca cttccaggat aaatttctcc aatccactca
     2941 aggccctgtc cagttctctg cttcacccaa cttataccat aacttgtgag ggtgtatcca
     3001 gaggccttgc aggacagctt cactgaggcc ccaggcctcg ccagctcagc tccagactgc
     3061 tgcagctgaa cctgggattg gacacctgca gttcctgaca ggatgaagag aaagatccag
     3121 atccattcca tgctagagcc aagcttgagc tttttgcaaa agcctaggcc tccaaaaaag
     3181 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
     3241 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
     3301 atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata
     3361 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc
     3421 atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac
     3481 acattccaca gtcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     3541 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     3601 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     3661 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc
     3721 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3781 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3841 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3901 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3961 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4021 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4081 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4141 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4201 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4261 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4321 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4381 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4441 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4501 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4561 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4621 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4681 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4741 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4801 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4861 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4921 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     4981 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5041 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5101 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5161 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5221 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     5281 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5341 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5401 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5461 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5521 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5581 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5641 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5701 acgaggccct ttcgtcttca agaa
//
