LOCUS       40924_41569        4200 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV23S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4200)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4200)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88887.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     promoter        complement(1629..1958)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(2373..2961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3135..3992)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3993..4097)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agagccaagc
     1621 ttgagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     1681 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     1741 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     1801 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     1861 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     1921 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagtcg ggcagcgttg
     1981 ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
     2041 ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
     2101 gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
     2161 cgtaaagtct ggaaacgcgg aagtcagcgc tcttccgctt cctcgctcac tgactcgctg
     2221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3421 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     3481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3721 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     3781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4141 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//
