LOCUS       V002994                 5297 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002994
VERSION     V002994
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5297)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88688.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1608..2004)
                     /label="3' UTR of XGPRT"
                     /note="3' UTR of XGPRT"
     CDS             complement(2008..2463)
                     /gene="gpt"
                     /label="Xanthine-guanine phosphoribosyltransferase"
                     /note="Xanthine-guanine phosphoribosyltransferase from
                     Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
                     Accession#: B7N8G9"
     misc_feature    complement(2464..2654)
                     /label="5' UTR of XGPRT"
                     /note="5' UTR of XGPRT"
     promoter        complement(2664..2993)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    3144..3284
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3470..4058)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4232..5089)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5090..5194)
                     /label="AmpR promoter"
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgggggtggc ccactcataa
     1621 atccagttgc cgccacggta gccaatcacc gtatcgtata aatcatcgtc ggtacgttcg
     1681 gcatcgctca tcacaatacg tgcctggacg tcgaggattt cgcgtgggtc aatgccgcgc
     1741 cagatccaca tcagacggtt aatcatgcga taccagtgag ggatggtttt accatcaagg
     1801 gccgactgca caggcggttg tgcgccgtga ttaaagcggc ggactagcgt cgaggtttca
     1861 ggatgtttaa agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt catggatgca
     1921 gcctccagaa tacttactgg aaactattgt aacccgcctg aagttaaaaa gaacaacgcc
     1981 cggcagtgcc aggcgttgaa aagattagcg accggagatt ggcgggacga atacgacgcc
     2041 catatcccac ggctgttcaa tccaggtatc ttgcgggata tcaacaacat agtcatcaac
     2101 cagcggacga ccagccggtt ttgcgaagat ggtgacaaag tgcgcttttg gatacatttc
     2161 acgaatcgca accgcagtac caccggtatc caccaggtca tcaataacga tgaagccttc
     2221 gccatcgcct tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt gatcgtagct
     2281 ggaaatacaa acggtatcga catgacgaat acccagttca cgcgccagta acgcacccgg
     2341 taccagaccg ccacggctta cggcaataat gcctttccat tgttcagaag gcatcagtcg
     2401 gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag gtgacgatgt atttttcgct
     2461 catgtgaagt gtcccagcct gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg
     2521 ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt atttcacatg agtccgcgtc
     2581 tttttacgca ctgcctctcc ctgacgcggg ataaagtggt attctcaaac atatctcgca
     2641 agcctgtctt gtgttccaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     2701 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     2761 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     2821 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     2881 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     2941 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     3001 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3061 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3121 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     3181 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     3241 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     3301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3901 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     4561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     4861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5221 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     5281 cctttcgtct tcaagaa
//
