LOCUS       40924_41554        4446 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSV009, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   de Vries SPW., Gupta S, Baig A.
  TITLE     Spontaneous mutations in Campylobacter jejuni strain M1: A 
            cautionary tale
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   de Vries SPW., Gupta S, Baig A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2015) Department of Veterinary Medicine, 
            University of Cambridge, Madingley Road, Cambridge CB3 0ES, United 
            Kingdom
REFERENCE   3  (bases 1 to 4446)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-AUG-2015) Department of Veterinary Medicine, University of 
            Cambridge, Madingley Road, Cambridge CB3 0ES, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(20..419)
                     /label=CjM1_0055 upstream (right flank)
                     /note="CjM1_0055 upstream (right flank)"
     primer_bind     22..46
                     /label=pSV009_GCamplif_RV1
                     /note="pSV009_GCamplif_RV1"
     CDS             complement(423..1214)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1215..1276)
                     /label=KAN promoter
                     /note="KAN promoter"
                     /regulatory_class="promoter"
     regulatory      complement(1328..1392)
                     /label=CAT promoter
                     /note="CAT promoter"
                     /regulatory_class="promoter"
     misc_feature    complement(1399..1798)
                     /label=CjM1_0055_0057 (left flank)
                     /note="CjM1_0055_0057 (left flank)"
     primer_bind     complement(1506..1526)
                     /label=pSV009_seq_FW1
                     /note="pSV009_seq_FW1"
     primer_bind     complement(1775..1798)
                     /label=pSV009_GCamplif_FW1
                     /note="pSV009_GCamplif_FW1"
     primer_bind     complement(1817..1833)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1975..2074)
                     /label=pBR322
                     /note="pBR322"
     misc_feature    complement(2075..2211)
                     /label=pBR322
                     /note="pBR322"
     promoter        2307..2411
                     /label=AmpR promoter
     CDS             2412..3269
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3443..4031
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4319..4340
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4355..4385
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4393..4409
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4417..4433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgcggc cgcatcgata gctattgcca tagtagctct tagtggcatt accactaccc
       61 aattgaaaat ctaagtaaaa tttacaccaa agctacttcg aaaacttcta cgcaacaact
      121 tattgatgag actaaaattt cctttaaata cttcagtact tgatttttta tatcaaaaga
      181 gtataagatg aaaaggtaaa tactgctata tcaaatttaa aatctaaaac gggtataaaa
      241 gacgctttaa gtggtttttc aaattctctt atttagctta tagttattct attattgtgg
      301 ctcgtggagt tattaaaaca ggacttggag agcatttggc ttatcatttt atcagtattc
      361 ttggtaaaaa aaccttaggt attgcttatt ctatagcttt ttgtgaaact attttagctc
      421 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
      481 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
      541 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
      601 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
      661 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
      721 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
      781 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
      841 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
      901 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
      961 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
     1021 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
     1081 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
     1141 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
     1201 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     1261 aagctaatta taacaagagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
     1321 cctcgagtta tcctccgtaa attccgattt gttgataact cattatatca taaatctatc
     1381 cactatatca tactcgagct ttattttctc tctccgctag aaattaaatc cacgcctaat
     1441 gcttctactt ttgctaaaga taaattaaaa gaacttgtaa aaatgaaaaa tagtgaaaaa
     1501 atcatgctca gtgtttttgt ctcgctttta cttttatggg ctggagcttt gggcttgttt
     1561 tttggaattt ctcttgatgc gactagtgtg gctttgcttg ggttgtcttt ggtgttgatt
     1621 agtggagttt taacttttgg ggaagttttg gccgaaaaag ctgcttggaa tactttagta
     1681 tggttttcag ctcttgtaat gatggcaact ttacttggca aacttggcgt tacacagttt
     1741 ttagctgatg gttaaggtga atttgcttct gctatgggac ttggggaaat ttctattagc
     1801 tagcccatgg gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     1861 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     1921 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     1981 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     2041 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     2101 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     2161 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     2221 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     2281 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2341 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2401 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2521 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2581 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2641 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2701 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2821 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2941 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3001 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3301 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4021 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4081 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4141 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4201 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4261 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4321 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     4381 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     4441 acgcca
//
