LOCUS       40924_41549        9657 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Eukaryotic vector pSV-ssTMbgyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Shirasawa S, Yoshimi M, Kamochi H, Muneta T, Kimura JH, Hook M, 
            Shinomura T.
  TITLE     Gene trap screening for cell surface and extracellular matrix 
            molecules produced by chondrocytes
  JOURNAL   J. Biochem. 137 (1), 79-85 (2005)
  PUBMED    15713886
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   Shinomura T, Shirasawa S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2004) Tamayuki Shinomura, Tokyo Medical and Dental
            University, Tissue Regeneration, Dept. of Hard Tissue Engineering; 
            1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan 
            (E-mail:t.shinomura.trg@tmd.ac.jp, Tel:81-3-5803-5450, 
            Fax:81-3-5803-0187)
REFERENCE   3  (bases 1 to 9657)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9657)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2005"; volume: "137"; issue: "1"; pages: "79-85"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2004) Tamayuki Shinomura, Tokyo Medical and Dental 
            University, Tissue Regeneration, Dept. of Hard Tissue Engineering"; 
            volume: " 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan 
            (E-mail:t.shinomura.trg@tmd.ac.jp, Tel:81-3-5803-5450, Fax"; pages: 
            "81-3-5803-0187"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        54..375
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          401..973
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1106..1150
                     /label=preprotrypsin leader
                     /note="leader sequence from mouse preprotrypsin"
     CDS             1151..1174
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    1231..1893
                     /gene="TM(CD4)"
                     /product="transmembrane domain of rat CD4"
     gene            1231..1893
                     /gene="TM(CD4)"
                     /label=TM(CD4)
     CDS             1901..4942
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             4958..5992
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    6037..6085
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      6276..6495
                     /regulatory_class="terminator"
     promoter        complement(6526..6544)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6562..6578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6586..6602)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6610..6640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6655..6676)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6964..7552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7726..8583)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8584..8688)
                     /label=AmpR promoter
     rep_origin      9022..9477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9618..9634
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9641..9657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcaagc ttgaggtgtg gcaggcttga gatcctgtgg aatgtgtgtc
       61 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      121 tcaattagtc agcaaggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      181 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      361 ctaggctttt gcaaaaagct cggatcctga gaacttcagg gtgagtttgg ggacccttga
      421 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
      481 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
      541 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
      601 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
      661 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
      721 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
      781 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
      841 catcctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
      901 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
      961 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     1021 agaattaatt caagcttggg ctgcaggtcg actctagagg atccccgggc gagctcgttt
     1081 agtgaaccgt cagaattaat tcaccatgtc tgcacttctg atcctagctc ttgttggagc
     1141 tgcagttgct gactacaaag acgatgacga caagcttgcg gccgcgaatt catcgataga
     1201 tcgatctgat atcggtaccg cgggccccat gggagagttg agatggaagg cagagaaggc
     1261 tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa
     1321 gtctaccagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc ttcagatacc
     1381 ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg acagagggat
     1441 actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca gcaacacttt
     1501 gacctgtgag gtgatgggac ctacctcacc caagatgaga ctgatcttga agcaggagaa
     1561 tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg
     1621 ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca agatccaggt
     1681 tttatccaaa gggttgaacc agacaatgtt cctggctgtc gtgctgggga gcgccttcag
     1741 ctttctggtt ttcacggggc tctgcatcct attctgtgtc aggtgccggc accaacagcg
     1801 ccaggcagca cggatgtctc agatcaagag gcttctcagt gagaagaaga cttgccagtg
     1861 ctcccaccgg atgcagaaaa gccacaatct catggatccc gtcgttttac aacgtcgtga
     1921 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1981 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2041 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
     2101 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta
     2161 cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc cgtttgttcc
     2221 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
     2281 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa
     2341 cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc
     2401 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga gtgacggcag
     2461 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct
     2521 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt
     2581 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg
     2641 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
     2701 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt
     2761 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact
     2821 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
     2881 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa
     2941 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
     3001 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
     3061 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat
     3121 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc
     3181 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
     3241 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat
     3301 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc
     3361 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt
     3421 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct
     3481 ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc
     3541 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct
     3601 gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac
     3661 gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc
     3721 agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac
     3781 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat
     3841 ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca
     3901 aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg
     3961 gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag
     4021 cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca
     4081 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg
     4141 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca
     4201 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt
     4261 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca
     4321 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct
     4381 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac
     4441 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga
     4501 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt
     4561 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt
     4621 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa
     4681 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt
     4741 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct
     4801 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg
     4861 cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg gtcgctacca
     4921 ttaccagttg gtctggtgtc aggggatcct cgccaccatg aaaaagcctg aactcaccgc
     4981 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     5041 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     5101 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     5161 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     5221 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     5281 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     5341 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     5401 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     5461 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     5521 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     5581 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     5641 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     5701 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     5761 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     5821 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     5881 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     5941 tggaaaccga cgccccagca ctcgtccgga tcgggagatg ggggaggcta actgaaacac
     6001 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     6061 cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     6121 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     6181 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     6241 cctgccatag ccactggccc cgtgggttag ggacggggtc ccccatgggg aatggtttat
     6301 ggttcgtggg ggttattatt ttgggcgttg cgtggggtca ggtccacgac tggactgagc
     6361 agacagaccc atggtttttg gatggcctgg gcatggaccg catgtactgg cgcgacacga
     6421 acaccgggcg tctgtggctg ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc
     6481 tggcgtgcca agctagtcga cctgcaggca tgcaagcttg agtattctat agtgtcacct
     6541 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6961 gtttttcgat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7381 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     7441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg gcattgctac
     8041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8281 aaccaagtca ttctgagaat accgcgcccg gcgaccgagt tgctcttgcc cggcgtcaat
     8341 acgggataat agtgtatgac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8701 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     8761 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     8821 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8881 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     8941 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     9001 gagaaaatac cgcatcaggc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9181 tccctttagg gttccgattt agagctttac ggcacctcga ccgcaaaaaa cttgatttgg
     9241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9421 agctgattta acaaatattt aacgcgaatt ttaacaaaat attaacgttt acaatttcca
     9481 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9541 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9601 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactata
//
