LOCUS       40924_41540        4105 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV-Sport-Rluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4105)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4105)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..949
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     promoter        complement(1042..1060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1149..1188
                     /label=intron SV40 small t-antigen
                     /note="intron SV40 small t-antigen"
     polyA_signal    1447..1581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1818..2396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2570..3427)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3428..3532)
                     /label=AmpR promoter
     promoter        3644..3675
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3683..3699
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        3734..4063
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        4069..4087
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 cggtccggaa ttcgccatgg cttcgaaagt ttatgatcca gaacaaagga aacggatgat
       61 aactggtccg cagtggtggg ccagatgtaa acaaatgaat gttcttgatt catttattaa
      121 ttattatgat tcagaaaaac atgcagaaaa tgctgttatt tttttacatg gtaacgcggc
      181 ctcttcttat ttatggcgac atgttgtgcc acatattgag ccagtagcgc ggtgtattat
      241 accagacctt attggtatgg gcaaatcagg caaatctggt aatggttctt ataggttact
      301 tgatcattac aaatatctta ctgcatggtt tgaacttctt aatttaccaa agaagatcat
      361 ttttgtcggc catgattggg gtgcttgttt ggcatttcat tatagctatg agcatcaaga
      421 taagatcaaa gcaatagttc acgctgaaag tgtagtagat gtgattgaat catgggatga
      481 atggcctgat attgaagaag atattgcgtt gatcaaatct gaagaaggag aaaaaatggt
      541 tttggagaat aacttcttcg tggaaaccat gttgccatca aaaatcatga gaaagttaga
      601 accagaagaa tttgcagcat atcttgaacc attcaaagag aaaggtgaag ttcgtcgtcc
      661 aacattatca tggcctcgtg aaatcccgtt agtaaaaggt ggtaaacctg acgttgtaca
      721 aattgttagg aattataatg cttatctacg tgcaagtgat gatttaccaa aaatgtttat
      781 tgaatcggac ccaggattct tttccaatgc tattgttgaa ggtgccaaga agtttcctaa
      841 tactgaattt gtcaaagtaa aaggtcttca tttttcgcaa gaagatgcac ctgatgaaat
      901 gggaaaatat atcaaatcgt tcgttgagcg agttctcaaa aatgaacaat aagaattccc
      961 gggtcgacga gctcactagt cggcggccgc tctagaggat ccaagcttac gtacgcgtgc
     1021 atgcgacgtc atagctctct ccctatagtg agtcgtatta taagctagct tgggatcttt
     1081 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1141 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactagc tgcatatgct
     1201 tgctgcttga gagttttgct tactgagtat gatttatgaa aatattatac acaggagcta
     1261 gtgattctaa ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc
     1321 agtcctcaca gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt
     1381 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1441 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1501 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1561 gtatcttatc atgtctggat cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1981 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     2041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2221 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcggc
     2281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2401 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2461 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2521 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2581 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2641 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2701 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2761 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2821 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2881 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2941 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3001 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3061 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3121 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3181 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3241 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3301 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3361 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3421 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3541 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     3601 ataggcgtat acgaggccct ttcactcatt aggcacccca ggctttacac tttatagctt
     3661 ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagcatc
     3721 gatgctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3781 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3901 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3961 cgtactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4021 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctat ttaggtgaca
     4081 ctatagaagg tacgcctgca ggtac
//
