LOCUS       V002998                 5246 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002998
VERSION     V002998
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5246)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..339
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        345..363
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             395..2338
                     /gene="RAF1"
                     /label="RAF proto-oncogene serine/threonine-protein kinase"
                     /note="RAF proto-oncogene serine/threonine-protein kinase
                     from Pongo abelii. Accession#: Q5R5M7"
     misc_feature    2348..2386
                     /label="E-tag"
                     /note="E-tag"
     promoter        complement(2564..2582)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2671..2710
                     /label="intron of SV40 small t-antigen"
                     /note="intron of SV40 small t-antigen"
     polyA_signal    2969..3103
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3340..3918)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4092..4949)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4950..5054)
                     /label="AmpR promoter"
     promoter        5166..5197
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    5205..5221
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat
      361 agaaggtacg cctgcaggta ccggtccgga attcatggag cacatacagg gagcttggaa
      421 gacgatcagc aatggttttg gattcaaaga tgccgtgttt gatggctcca gctgcatctc
      481 tcctacaata gttcagcagt ttggctatca gcgccgggca tcagatgatg gcaaactcac
      541 agatccttct aagacaagca acactatccg tgttttcttg ccgaacaagc aaagaacagt
      601 ggtcaatgtg cgaaatggaa tgagcttgca tgactgcctt atgaaagcac tcaaggtgag
      661 gggcctgcaa ccagagtgct gtgcagtgtt cagacttctc cacgaacaca aaggtaaaaa
      721 agcacgctta gattggaata ctgatgctgc gtctttgatt ggagaagaac ttcaagtaga
      781 tttcctggat catgttcccc tcacaacaca caactttgct cggaagacgt tcctgaagct
      841 tgccttctgt gacatctgtc agaaattcct gctcaatgga tttcgatgtc agacttgtgg
      901 ctacaaattt catgagcact gtagcaccaa agtacctact atgtgtgtgg actggagtaa
      961 catcagacaa ctcttattgt ttccaaattc cactattggt gatagtggag tcccagcact
     1021 accttctttg actatgcgtc gtatgcgaga gtctgtttcc aggatgcctg ttagttctca
     1081 gcacagatat tctacacctc acgccttcac ctttaacacc tccagtccct catctgaagg
     1141 ttccctctcc cagaggcaga ggtcgacatc cacacctaat gtccacatgg tcagcaccac
     1201 cctgcctgtg gacagcagga tgattgagga tgcaattcga agtcacagcg aatcagcctc
     1261 accttcagcc ctgtccagta gccccaacaa tctgagccca acaggctggt cacagccgaa
     1321 aacccccgtg ccagcacaaa gagagcgggc accagtatct gggacccagg agaaaaacaa
     1381 aattaggcct cgtggacaga gagattcaag ctattattgg gaaatagaag ccagtgaagt
     1441 gatgctgtcc actcggattg ggtcaggctc ttttggaact gtttataagg gtaaatggca
     1501 cggagatgtt gcagtaaaga tcctaaaggt tgtcgaccca accccagagc aattccaggc
     1561 cttcaggaat gaggtggctg ttctgcgcaa aacacggcat gtgaacattc tgcttttcat
     1621 ggggtacatg acaaaggaca acctggcaat tgtgacccag tggtgcgagg gcagcagcct
     1681 ctacaaacac ctgcatgtcc aggagaccaa gtttcagatg ttccagctaa ttgacattgc
     1741 ccggcagacg gctcagggaa tggactattt gcatgcaaag aacatcatcc atagagacat
     1801 gaaatccaac aatatatttc tccatgaagg cttaacagtg aaaattggag attttggttt
     1861 ggcaacagta aagtcacgct ggagtggttc tcagcaggtt gaacaaccta ctggctctgt
     1921 cctctggatg gccccagagg tgatccgaat gcaggataac aacccattca gtttccagtc
     1981 ggatgtctac tcctatggca tcgtattgta tgaactgatg acgggggagc ttccttattc
     2041 tcacatcaac aaccgagatc agatcatctt catggtgggc cgaggatatg cctccccaga
     2101 tcttagtaag ctatataaga actgccccaa agcaatgaag aggctggtag ctgactgtgt
     2161 gaagaaagta aaggaagaga ggcctctttt tccccagatc ctgtcttcca ttgagctgct
     2221 ccaacactct ctaccgaaga tcaaccggag cgcttccgag ccatccttgc atcgggcagc
     2281 ccacactgag gatatcaatg cttgcacgct gaccacgtcc ccgaggctgc ctgtcttcgc
     2341 ggccgcaggt gcgccggtgc cgtatccgga tccgctggaa ccgcgtgccg catagactgt
     2401 tgaaagttgt ttagcaaaac ctcatacaga aaattcattt actaacgtct ggaaagacga
     2461 caaaacttta gatcgttacg ctaactatga gggctgtctg tggaatgtcg actctagagg
     2521 atccaagctt acgtacgcgt gcatgcgacg tcatagctct ctccctatag tgagtcgtat
     2581 tataagctag cttgggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     2641 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     2701 tgttaaacta gctgcatatg cttgctgctt gagagttttg cttactgagt atgatttatg
     2761 aaaatattat acacaggagc tagtgattct aattgtttgt gtattttaga ttcacagtcc
     2821 caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa tcagccatac
     2881 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2941 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3001 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3061 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcctgcatt aatgaatcgg
     3121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     3541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3781 gacagtattt ggtatctgcg gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     5101 atcatgacat taacctataa aaataggcgt atacgaggcc ctttcactca ttaggcaccc
     5161 caggctttac actttatagc ttccggctcg tataatgtgt ggaattgtga gcggataaca
     5221 atttcacaca ggaaacagca tcgatg
//
