LOCUS       40924_41530        5612 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV-Sport-PITSLRE-E, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5612)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        345..363
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             404..2704
                     /codon_start=1
                     /note="unnamed protein product; p110 PITSLRE"
                     /protein_id="SJL87149.1"
                     /translation="MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKR
                     DSLEEGELRDHCMEITIRNSPYRREDSMEDRGEEDDSLAIKPPQQMSWKEKVHHRKDEK
                     RKEKWKHARVKEREHERRKRHREEQDKARREWERQKRREMAREHSRRERDRLEQLERKR
                     ERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMREDYSDKVKASHWSRS
                     PPRPPRERFELGDGRKPVKEEKMEERDLLSDLQDISDSERKTSSAESSSAESGSGSEEE
                     EEEEEEEEEEGSTSEEPEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEERENENH
                     LLVVPESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALLPIELKQELPKYLP
                     ALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREI
                     NTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQ
                     LLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVVVTQWYR
                     APELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGNSEIDQINKVFKELGTPSEKIWP
                     GYSELPVVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEY
                     FRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTN
                     QGASAAGPGFSLKF"
     misc_feature    2714..2752
                     /label=E-tag
                     /note="E-tag"
     promoter        complement(2930..2948)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3037..3076
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     polyA_signal    3335..3469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3706..4284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4458..5315)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5316..5420)
                     /label=AmpR promoter
     promoter        5532..5563
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    5571..5587
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat
      361 agaaggtacg cctgcaggta ccggtccgga attcttaact caaatgggtg atgaaaagga
      421 ctcttggaaa gtgaaaactt tagatgaaat tcttcaggaa aagaaacgaa ggaaggaaca
      481 agaggagaaa gcagagataa aacgcttaaa aaattctgat gaccgggatt ccaagcggga
      541 ttcccttgag gagggggagc tgagagatca ctgcatggag atcacaataa ggaactcccc
      601 gtatagaaga gaagactcaa tggaagacag aggagaagaa gatgattctt tggccatcaa
      661 accaccccag caaatgtctt ggaaagaaaa agttcatcac agaaaagatg aaaagaggaa
      721 agaaaaatgg aagcatgcta gagtgaaaga aagagagcac gaacgtcgga aacgacatcg
      781 agaagaacag gataaagctc gccgggaatg ggaaagacag aagagaaggg aaatggcaag
      841 ggagcattcc aggagagaaa gggaccgctt ggagcagtta gaaaggaagc gggagcggga
      901 gcgcaagatg cgggagcagc agaaggagca gcgggagcag aaggagcgcg agcggcgggc
      961 ggaggagcgg cgcaaggagc gggaggcccg cagggaagtg tctgcacatc accgaacgat
     1021 gagagaggac tacagcgaca aagtgaaagc cagccactgg agtcgcagcc cgcctcggcc
     1081 gccgcgggag cggttcgagt tgggagacgg ccggaagcca gtaaaagaag agaaaatgga
     1141 agaaagggac ctgctgtccg acttacagga catcagcgac agcgagagga agaccagctc
     1201 ggccgagtcc tcgtcagcag aatcaggctc aggttctgag gaagaagagg aggaggagga
     1261 agaggaggag gaggaaggga gcaccagtga agaaccagag gaggaagagg aagaggagga
     1321 ggaggagacc ggcagcaact ctgaggaggc atcagagcag tctgccgaag aagtaagtga
     1381 ggaagaaatg agtgaagatg aagaacgaga aaatgaaaac cacctcttgg ttgttccaga
     1441 gtcacggttc gaccgagatt ccggggagag tgaagaagca gaggaagaag tgggtgaggg
     1501 aacgccgcag agcagcgccc tgacagaggg cgactatgtg cccgactccc ctgccctgtt
     1561 gcccatcgag ctcaagcagg agctgcccaa gtacctgccg gccctgcagg gctgccggag
     1621 cgtcgaggag ttccagtgcc tgaacaggat cgaggagggc acctatggag tggtctacag
     1681 agcaaaagac aagaaaacag atgaaattgt ggctctaaag cggctgaaga tggagaagga
     1741 gaaggagggc ttcccgatca cgtccctgag ggagatcaac accatcctca aggcccagca
     1801 tcccaacatt gtcaccgtta gagagattgt ggtgggcagc aacatggaca agatctacat
     1861 cgtgatgaac tatgtggagc acgacctcaa gagcctgatg gagaccatga aacagccctt
     1921 cctgccaggg gaggtgaaga ccctgatgat ccagctgctg cgtggggtga aacacctgca
     1981 cgacaactgg atcctgcacc gtgacctcaa gacgtccaac ctgctgctga gccacgccgg
     2041 catcctcaag gtgggtgatt ttgggctggc gcgggagtac ggatcccctc tgaaggccta
     2101 caccccggtc gtggtgaccc agtggtaccg cgccccagag ctgctgcttg gtgccaagga
     2161 atactccacg gccgtggaca tgtggtcagt gggctgcatc ttcggggagc tgctgactca
     2221 gaagcctctg ttccccggga attcggaaat cgatcagatc aacaaagtgt tcaaggagct
     2281 ggggaccccc agtgagaaaa tctggcccgg ctacagtgag ctcccagtag tcaagaagat
     2341 gaccttcagc gagcacccct acaacaacct ccgcaagcgc ttcggggctc tgctctcaga
     2401 ccagggcttc gacctcatga acaagttcct gacctacttc cccgggagga ggatcagcgc
     2461 tgaggacggc ctcaagcatg agtatttccg cgagaccccc ctccccatcg acccctccat
     2521 gttccccacg tggcccgcca agagcgagca gcagcgtgtg aagcggggca ccagcccgag
     2581 gccccctgag ggaggcctgg gctacagcca gctgggtgac gacgacctga aggagacggg
     2641 cttccacctt accaccacga accagggggc ctctgccgcg ggccccggct tcagcctcaa
     2701 gttcgcggcc gcaggtgcgc cggtgccgta tccggatccg ctggaaccgc gtgccgcata
     2761 gactgttgaa agttgtttag caaaacctca tacagaaaat tcatttacta acgtctggaa
     2821 agacgacaaa actttagatc gttacgctaa ctatgagggc tgtctgtgga atgtcgactc
     2881 tagaggatcc aagcttacgt acgcgtgcat gcgacgtcat agctctctcc ctatagtgag
     2941 tcgtattata agctagcttg ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     3001 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     3061 ataatgtgtt aaactagctg catatgcttg ctgcttgaga gttttgctta ctgagtatga
     3121 tttatgaaaa tattatacac aggagctagt gattctaatt gtttgtgtat tttagattca
     3181 cagtcccaag gctcatttca ggcccctcag tcctcacagt ctgttcatga tcataatcag
     3241 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3301 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3361 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3421 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc tgcattaatg
     3481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3901 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4141 tagaaggaca gtatttggta tctgcggcca gttaccttcg gaaaaagagt tggtagctct
     4201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4321 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4381 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4441 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4501 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4561 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4621 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4681 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4741 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4801 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4861 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4921 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4981 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5041 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5101 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5161 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5221 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5281 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5401 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     5461 attattatca tgacattaac ctataaaaat aggcgtatac gaggcccttt cactcattag
     5521 gcaccccagg ctttacactt tatagcttcc ggctcgtata atgtgtggaa ttgtgagcgg
     5581 ataacaattt cacacaggaa acagcatcga tg
//
